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Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats

Male Unib-WH rats were pretreated for two weeks with butanolic (BuOH) and ethyl acetate (EtOAc) fractions. Colitis was induced by rectal administration of TNBS, the treatment continued, and animals were sacrificed on day 7 after the TNBS administration. Phytochemical studies were performed in order...

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Autores principales: de Faria, Felipe Meira, Luiz-Ferreira, Anderson, Socca, Eduardo Augusto Rabelo, de Almeida, Ana Cristina Alves, Dunder, Ricardo José, Manzo, Luis Paulo, da Silva, Marcelo Aparecido, Vilegas, Wagner, Rozza, Ariane Leite, Pellizzon, Cláudia Helena, dos Santos, Lourdes Campaner, Souza Brito, Alba Regina Monteiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465987/
https://www.ncbi.nlm.nih.gov/pubmed/23056142
http://dx.doi.org/10.1155/2012/753971
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author de Faria, Felipe Meira
Luiz-Ferreira, Anderson
Socca, Eduardo Augusto Rabelo
de Almeida, Ana Cristina Alves
Dunder, Ricardo José
Manzo, Luis Paulo
da Silva, Marcelo Aparecido
Vilegas, Wagner
Rozza, Ariane Leite
Pellizzon, Cláudia Helena
dos Santos, Lourdes Campaner
Souza Brito, Alba Regina Monteiro
author_facet de Faria, Felipe Meira
Luiz-Ferreira, Anderson
Socca, Eduardo Augusto Rabelo
de Almeida, Ana Cristina Alves
Dunder, Ricardo José
Manzo, Luis Paulo
da Silva, Marcelo Aparecido
Vilegas, Wagner
Rozza, Ariane Leite
Pellizzon, Cláudia Helena
dos Santos, Lourdes Campaner
Souza Brito, Alba Regina Monteiro
author_sort de Faria, Felipe Meira
collection PubMed
description Male Unib-WH rats were pretreated for two weeks with butanolic (BuOH) and ethyl acetate (EtOAc) fractions. Colitis was induced by rectal administration of TNBS, the treatment continued, and animals were sacrificed on day 7 after the TNBS administration. Phytochemical studies were performed in order to provide the characterization of the tannins present in the bark of R. mangle. Results showed that EtOAc fraction increased the levels of IL-10 (∗∗P < 0.01) and diminished the levels of TNF-α (∗∗∗P < 0.001) and IL-6 (∗∗P < 0.01). BuOH fraction reduced the MPO activity (∗∗P < 0.01) and levels of TBARS (∗∗∗P < 0.001); it also increased COX-1 expression, diminished the levels of TNF-α (∗∗∗P < 0.001), and increased the levels of IL-12 (∗∗∗P < 0.001). Besides, both treatments augmented the levels of GSH (∗P < 0.05), the activity of GSH-Px (∗∗P < 0.01 for BuOH fraction and ∗∗∗P < 0.001 for EtOAc fraction), and CAT (∗∗P < 0.01). In conclusion, both treatments ameliorated the injury induced by TNBS through different mechanisms, probably by their chemical composition which directed its activity into an antioxidant or anti-inflammatory response, leading to an immune modulation.
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spelling pubmed-34659872012-10-10 Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats de Faria, Felipe Meira Luiz-Ferreira, Anderson Socca, Eduardo Augusto Rabelo de Almeida, Ana Cristina Alves Dunder, Ricardo José Manzo, Luis Paulo da Silva, Marcelo Aparecido Vilegas, Wagner Rozza, Ariane Leite Pellizzon, Cláudia Helena dos Santos, Lourdes Campaner Souza Brito, Alba Regina Monteiro Evid Based Complement Alternat Med Research Article Male Unib-WH rats were pretreated for two weeks with butanolic (BuOH) and ethyl acetate (EtOAc) fractions. Colitis was induced by rectal administration of TNBS, the treatment continued, and animals were sacrificed on day 7 after the TNBS administration. Phytochemical studies were performed in order to provide the characterization of the tannins present in the bark of R. mangle. Results showed that EtOAc fraction increased the levels of IL-10 (∗∗P < 0.01) and diminished the levels of TNF-α (∗∗∗P < 0.001) and IL-6 (∗∗P < 0.01). BuOH fraction reduced the MPO activity (∗∗P < 0.01) and levels of TBARS (∗∗∗P < 0.001); it also increased COX-1 expression, diminished the levels of TNF-α (∗∗∗P < 0.001), and increased the levels of IL-12 (∗∗∗P < 0.001). Besides, both treatments augmented the levels of GSH (∗P < 0.05), the activity of GSH-Px (∗∗P < 0.01 for BuOH fraction and ∗∗∗P < 0.001 for EtOAc fraction), and CAT (∗∗P < 0.01). In conclusion, both treatments ameliorated the injury induced by TNBS through different mechanisms, probably by their chemical composition which directed its activity into an antioxidant or anti-inflammatory response, leading to an immune modulation. Hindawi Publishing Corporation 2012 2012-09-27 /pmc/articles/PMC3465987/ /pubmed/23056142 http://dx.doi.org/10.1155/2012/753971 Text en Copyright © 2012 Felipe Meira de Faria et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
de Faria, Felipe Meira
Luiz-Ferreira, Anderson
Socca, Eduardo Augusto Rabelo
de Almeida, Ana Cristina Alves
Dunder, Ricardo José
Manzo, Luis Paulo
da Silva, Marcelo Aparecido
Vilegas, Wagner
Rozza, Ariane Leite
Pellizzon, Cláudia Helena
dos Santos, Lourdes Campaner
Souza Brito, Alba Regina Monteiro
Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
title Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
title_full Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
title_fullStr Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
title_full_unstemmed Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
title_short Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
title_sort effects of rhizophora mangle on experimental colitis induced by tnbs in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465987/
https://www.ncbi.nlm.nih.gov/pubmed/23056142
http://dx.doi.org/10.1155/2012/753971
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