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Ratiometric imaging of calcium during ischemia-reperfusion injury in isolated mouse hearts using Fura-2
BACKGROUND: We present an easily implementable method for measuring Fura-2 fluorescence from isolated mouse hearts using a commercially available switching light source and CCD camera. After calibration, it provides a good estimate of intracellular [Ca(2+)] with both high spatial and temporal resolu...
Autores principales: | Venkataraman, Raghav, Holcomb, Mark R, Harder, Rene, Knollmann, Björn C, Baudenbacher, Franz |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466138/ https://www.ncbi.nlm.nih.gov/pubmed/22812644 http://dx.doi.org/10.1186/1475-925X-11-39 |
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