Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF

The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we repor...

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Detalles Bibliográficos
Autores principales: Funken, Horst, Bartels, Kai-Malte, Wilhelm, Susanne, Brocker, Melanie, Bott, Michael, Bains, Manjeet, Hancock, Robert E. W., Rosenau, Frank, Jaeger, Karl-Erich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466170/
https://www.ncbi.nlm.nih.gov/pubmed/23056489
http://dx.doi.org/10.1371/journal.pone.0046857
Descripción
Sumario:The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.

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