Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF

The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we repor...

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Autores principales: Funken, Horst, Bartels, Kai-Malte, Wilhelm, Susanne, Brocker, Melanie, Bott, Michael, Bains, Manjeet, Hancock, Robert E. W., Rosenau, Frank, Jaeger, Karl-Erich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466170/
https://www.ncbi.nlm.nih.gov/pubmed/23056489
http://dx.doi.org/10.1371/journal.pone.0046857
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author Funken, Horst
Bartels, Kai-Malte
Wilhelm, Susanne
Brocker, Melanie
Bott, Michael
Bains, Manjeet
Hancock, Robert E. W.
Rosenau, Frank
Jaeger, Karl-Erich
author_facet Funken, Horst
Bartels, Kai-Malte
Wilhelm, Susanne
Brocker, Melanie
Bott, Michael
Bains, Manjeet
Hancock, Robert E. W.
Rosenau, Frank
Jaeger, Karl-Erich
author_sort Funken, Horst
collection PubMed
description The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.
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spelling pubmed-34661702012-10-10 Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF Funken, Horst Bartels, Kai-Malte Wilhelm, Susanne Brocker, Melanie Bott, Michael Bains, Manjeet Hancock, Robert E. W. Rosenau, Frank Jaeger, Karl-Erich PLoS One Research Article The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF. Public Library of Science 2012-10-08 /pmc/articles/PMC3466170/ /pubmed/23056489 http://dx.doi.org/10.1371/journal.pone.0046857 Text en © 2012 Funken et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Funken, Horst
Bartels, Kai-Malte
Wilhelm, Susanne
Brocker, Melanie
Bott, Michael
Bains, Manjeet
Hancock, Robert E. W.
Rosenau, Frank
Jaeger, Karl-Erich
Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF
title Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF
title_full Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF
title_fullStr Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF
title_full_unstemmed Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF
title_short Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF
title_sort specific association of lectin lecb with the surface of pseudomonas aeruginosa: role of outer membrane protein oprf
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466170/
https://www.ncbi.nlm.nih.gov/pubmed/23056489
http://dx.doi.org/10.1371/journal.pone.0046857
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