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Fluorescence of Alexa Fluor Dye Tracks Protein Folding

Fluorescence spectroscopy is an important tool for the characterization of protein folding. Often, a protein is labeled with appropriate fluorescent donor and acceptor probes and folding-induced changes in Förster Resonance Energy Transfer (FRET) are monitored. However, conformational changes of the...

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Detalles Bibliográficos
Autores principales: Lindhoud, Simon, Westphal, Adrie H., Visser, Antonie J. W. G., Borst, Jan Willem, van Mierlo, Carlo P. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466183/
https://www.ncbi.nlm.nih.gov/pubmed/23056480
http://dx.doi.org/10.1371/journal.pone.0046838