Cargando…

Simple Monitoring of Gene Targeting Efficiency in Human Somatic Cell Lines Using the PIGA Gene

Gene targeting in most of human somatic cell lines has been labor-intensive because of low homologous recombination efficiency. The development of an experimental system that permits a facile evaluation of gene targeting efficiency in human somatic cell lines is the first step towards the improvemen...

Descripción completa

Detalles Bibliográficos
Autores principales:	Karnan, Sivasundaram, Konishi, Yuko, Ota, Akinobu, Takahashi, Miyuki, Damdindorj, Lkhagvasuren, Hosokawa, Yoshitaka, Konishi, Hiroyuki
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466256/ https://www.ncbi.nlm.nih.gov/pubmed/23056640 http://dx.doi.org/10.1371/journal.pone.0047389

Ejemplares similares

A Comparative Analysis of Constitutive Promoters Located in Adeno-Associated Viral Vectors
por: Damdindorj, Lkhagvasuren, et al.
Publicado: (2014)

Improved methods of AAV-mediated gene targeting for human cell lines using ribosome-skipping 2A peptide
por: Karnan, Sivasundaram, et al.
Publicado: (2016)

Flow cytometry-based quantification of targeted knock-in events in human cell lines using a GPI-anchor biosynthesis gene PIGP
por: Rahman, Md. Lutfur, et al.
Publicado: (2021)

Flow cytometry-based quantification of genome editing efficiency in human cell lines using the L1CAM gene
por: Hasan, Muhammad Nazmul, et al.
Publicado: (2023)

Novel ATP‐competitive Akt inhibitor afuresertib suppresses the proliferation of malignant pleural mesothelioma cells
por: Yamaji, Masayuki, et al.
Publicado: (2017)

Experimental strategies to achieve efficient targeted knock-in via tandem paired nicking
por: Rahman, Md. Lutfur, et al.
Publicado: (2021)

Establishment and characterization of CRISPR/Cas9‐mediated NF2 (−/−) human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma
por: Wahiduzzaman, Md, et al.
Publicado: (2018)

Novel Interleukin-6 Inducible Gene PDZ-Binding Kinase Promotes Tumor Growth of Multiple Myeloma Cells
por: Ota, Akinobu, et al.
Publicado: (2020)

Biallelic loss of FAM46C triggers tumor growth with concomitant activation of Akt signaling in multiple myeloma cells
por: Kanasugi, Jo, et al.
Publicado: (2020)

BCL6 inhibition ameliorates resistance to ruxolitinib in CRLF2-rearranged acute lymphoblastic leukemia
por: Tsuzuki, Shinobu, et al.
Publicado: (2022)

Discovery of novel molecular characteristics and cellular biological properties in ameloblastoma
por: Kondo, Sayuri, et al.
Publicado: (2020)

Identification of CD24 as a potential diagnostic and therapeutic target for malignant pleural mesothelioma
por: Karnan, Sivasundaram, et al.
Publicado: (2020)

Δ40p53α suppresses tumor cell proliferation and induces cellular senescence in hepatocellular carcinoma cells
por: Ota, Akinobu, et al.
Publicado: (2017)

CAMK2D: a novel molecular target for BAP1-deficient malignant mesothelioma
por: Karnan, Sivasundaram, et al.
Publicado: (2023)

CD52 is a novel target for the treatment of FLT3-ITD-mutated myeloid leukemia
por: Karnan, Sivasundaram, et al.
Publicado: (2021)

Identification of cisplatin-resistant factor by integration of transcriptomic and proteomic data using head and neck carcinoma cell lines
por: Inukai, Daisuke, et al.
Publicado: (2020)

Inhibition of VEGFR2 and EGFR signaling cooperatively suppresses the proliferation of oral squamous cell carcinoma
por: Onda, Maho, et al.
Publicado: (2023)

The genotypic and phenotypic spectrum of PIGA deficiency
por: Tarailo-Graovac, Maja, et al.
Publicado: (2015)

Gut microbiota alternation under the intestinal epithelium-specific knockout of mouse Piga gene
por: Jangid, Aditi, et al.
Publicado: (2022)

Minor GPI(-) granulocyte populations in aplastic anemia and healthy individuals derived from a few PIGA-mutated hematopoietic stem progenitor cells
por: Mizumaki, Hiroki, et al.
Publicado: (2023)

Drosophila models of PIGA-CDG mirror patient phenotypes
por: Thorpe, Holly J., et al.
Publicado: (2023)

Establishment and characterization of a novel vincristine‐resistant diffuse large B‐cell lymphoma cell line containing the 8q24 homogeneously staining region
por: Mizuno, Shohei, et al.
Publicado: (2018)

TSPO PIGA Ligands Promote Neurosteroidogenesis and Human Astrocyte Well-Being
por: Da Pozzo, Eleonora, et al.
Publicado: (2016)

Application of counter-selectable marker PIGA in engineering designer deletion cell lines and characterization of CRISPR deletion efficiency
por: Li, Donghui, et al.
Publicado: (2021)

Constitutional PIGA mutations cause a novel subtype of hemochromatosis in patients with neurologic dysfunction
por: Muckenthaler, Lena, et al.
Publicado: (2022)

Somatic Mutation of the APC Gene in Thyroid Carcinoma Associated with Familial Adenomatous Polyposis
por: Iwama, Takeo, et al.
Publicado: (1999)

Clonal Cell Proliferation in Paroxysmal Nocturnal Hemoglobinuria: Evaluation of PIGA Mutations and T-cell Receptor Clonality
por: Park, Joonhong, et al.
Publicado: (2019)

Chromosomal translocation t(11;14) and p53 deletion induced by the CRISPR/Cas9 system in normal B cell-derived iPS cells
por: Azami, Yusuke, et al.
Publicado: (2021)

A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model
por: Yuan, Xuan, et al.
Publicado: (2017)

A conditional counterselectable Piga knockout in mouse embryonic stem cells for advanced genome writing applications
por: Zhang, Weimin, et al.
Publicado: (2022)

A simple algorithm for QCD jets
por: Konishi, K
Publicado: (1978)

Cytotoxicity of Callerya speciosa Fractions against Myeloma and Lymphoma Cell Lines
por: Lam, Vu Quang, et al.
Publicado: (2022)

Fes-Cre Targets Phosphatidylinositol Glycan Class a (Piga) Inactivation to Hematopoietic Stem Cells in the Bone Marrow
por: Keller, Peter, et al.
Publicado: (2001)

Obstructive Sleep Apnea in a Clinical Population: Prevalence, Predictive Factors, and Clinical Characteristics of Patients Referred to a Sleep Center in Mongolia
por: Dashzeveg, Shuren, et al.
Publicado: (2021)

An Improved Online Self-Calibration Method Utilizing Angular Velocity Observation for Ultra High Accuracy PIGA-Based IMU
por: Zhang, Yongfeng, et al.
Publicado: (2022)

Pancreas-Brain Crosstalk
por: Lkhagvasuren, Battuvshin, et al.
Publicado: (2021)

Array-comparative genomic hybridization profiling of immunohistochemical subgroups of diffuse large B-cell lymphoma shows distinct genomic alterations
por: Guo, Ying, et al.
Publicado: (2014)

Propofol elicits apoptosis and attenuates cell growth in esophageal cancer cell lines
por: Zhou, Rui, et al.
Publicado: (2023)

A simple algorithm for QCD jets
por: Konishi, K I, et al.
Publicado: (1978)

MetaPIGA v2.0: maximum likelihood large phylogeny estimation using the metapopulation genetic algorithm and other stochastic heuristics
por: Helaers, Raphaël, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_f90hrnb0qfsub91k12f7t4qrbm