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Pyocyanin Promotes Extracellular DNA Release in Pseudomonas aeruginosa
Bacterial adhesion and biofilm formation are both dependent on the production of extracellular polymeric substances (EPS) mainly composed of polysaccharides, proteins, lipids, and extracellular DNA (eDNA). eDNA promotes biofilm establishment in a wide range of bacterial species. In Pseudomonas aerug...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466280/ https://www.ncbi.nlm.nih.gov/pubmed/23056420 http://dx.doi.org/10.1371/journal.pone.0046718 |
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author | Das, Theerthankar Manefield, Mike |
author_facet | Das, Theerthankar Manefield, Mike |
author_sort | Das, Theerthankar |
collection | PubMed |
description | Bacterial adhesion and biofilm formation are both dependent on the production of extracellular polymeric substances (EPS) mainly composed of polysaccharides, proteins, lipids, and extracellular DNA (eDNA). eDNA promotes biofilm establishment in a wide range of bacterial species. In Pseudomonas aeruginosa eDNA is major component of biofilms and is essential for biofilm formation and stability. In this study we report that production of pyocyanin in P. aeruginosa PAO1 and PA14 batch cultures is responsible for promotion of eDNA release. A phzSH mutant of P. aeruginosa PAO1 that overproduces pyocyanin displayed enhanced hydrogen peroxide (H(2)O(2)) generation, cell lysis, and eDNA release in comparison to its wildtype strain. A ΔphzA-G mutant of P. aeruginosa PA14 deficient in pyocyanin production generated negligible amounts of H(2)O(2) and released less eDNA in comparison to its wildtype counterpart. Exogenous addition of pyocyanin or incubation with H(2)O(2) was also shown to promote eDNA release in low pyocyanin producing (PAO1) and pyocynain deficient (PA14) strains. Based on these data and recent findings in the biofilm literature, we propose that the impact of pyocyanin on biofilm formation in P. aeruginosa occurs via eDNA release through H(2)O(2) mediated cell lysis. |
format | Online Article Text |
id | pubmed-3466280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34662802012-10-10 Pyocyanin Promotes Extracellular DNA Release in Pseudomonas aeruginosa Das, Theerthankar Manefield, Mike PLoS One Research Article Bacterial adhesion and biofilm formation are both dependent on the production of extracellular polymeric substances (EPS) mainly composed of polysaccharides, proteins, lipids, and extracellular DNA (eDNA). eDNA promotes biofilm establishment in a wide range of bacterial species. In Pseudomonas aeruginosa eDNA is major component of biofilms and is essential for biofilm formation and stability. In this study we report that production of pyocyanin in P. aeruginosa PAO1 and PA14 batch cultures is responsible for promotion of eDNA release. A phzSH mutant of P. aeruginosa PAO1 that overproduces pyocyanin displayed enhanced hydrogen peroxide (H(2)O(2)) generation, cell lysis, and eDNA release in comparison to its wildtype strain. A ΔphzA-G mutant of P. aeruginosa PA14 deficient in pyocyanin production generated negligible amounts of H(2)O(2) and released less eDNA in comparison to its wildtype counterpart. Exogenous addition of pyocyanin or incubation with H(2)O(2) was also shown to promote eDNA release in low pyocyanin producing (PAO1) and pyocynain deficient (PA14) strains. Based on these data and recent findings in the biofilm literature, we propose that the impact of pyocyanin on biofilm formation in P. aeruginosa occurs via eDNA release through H(2)O(2) mediated cell lysis. Public Library of Science 2012-10-08 /pmc/articles/PMC3466280/ /pubmed/23056420 http://dx.doi.org/10.1371/journal.pone.0046718 Text en © 2012 Das, Manefield http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Das, Theerthankar Manefield, Mike Pyocyanin Promotes Extracellular DNA Release in Pseudomonas aeruginosa |
title | Pyocyanin Promotes Extracellular DNA Release in Pseudomonas aeruginosa
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title_full | Pyocyanin Promotes Extracellular DNA Release in Pseudomonas aeruginosa
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title_fullStr | Pyocyanin Promotes Extracellular DNA Release in Pseudomonas aeruginosa
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title_full_unstemmed | Pyocyanin Promotes Extracellular DNA Release in Pseudomonas aeruginosa
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title_short | Pyocyanin Promotes Extracellular DNA Release in Pseudomonas aeruginosa
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title_sort | pyocyanin promotes extracellular dna release in pseudomonas aeruginosa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466280/ https://www.ncbi.nlm.nih.gov/pubmed/23056420 http://dx.doi.org/10.1371/journal.pone.0046718 |
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