Cargando…
PET Analysis of Dopaminergic Neurodegeneration in Relation to Immobility in the MPTP-Treated Common Marmoset, a Model for Parkinson’s Disease
BACKGROUND: Positron Emission Tomography (PET) measurement was applied to the brain of the common marmoset, a small primate species, treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). The marmoset shows prominent Parkinson’s disease (PD) signs due to dopaminergic neural degeneration....
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466292/ https://www.ncbi.nlm.nih.gov/pubmed/23056291 http://dx.doi.org/10.1371/journal.pone.0046371 |
_version_ | 1782245668556898304 |
---|---|
author | Ando, Kiyoshi Obayashi, Shigeru Nagai, Yuji Oh-Nishi, Arata Minamimoto, Takafumi Higuchi, Makoto Inoue, Takashi Itoh, Toshio Suhara, Tetsuya |
author_facet | Ando, Kiyoshi Obayashi, Shigeru Nagai, Yuji Oh-Nishi, Arata Minamimoto, Takafumi Higuchi, Makoto Inoue, Takashi Itoh, Toshio Suhara, Tetsuya |
author_sort | Ando, Kiyoshi |
collection | PubMed |
description | BACKGROUND: Positron Emission Tomography (PET) measurement was applied to the brain of the common marmoset, a small primate species, treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). The marmoset shows prominent Parkinson’s disease (PD) signs due to dopaminergic neural degeneration. Recently, the transgenic marmoset (TG) carrying human PD genes is developing. For phenotypic evaluations of TG, non-invasive PET measurement is considered to be substantially significant. As a reference control for TG, the brain of the MPTP-marmoset as an established and valid model was scanned by PET. Behavioral analysis was also performed by recording locomotion of the MPTP-marmoset, as an objective measure of PD signs. METHODOLOGY/PRINCIPAL FINDINGS: Marmosets received several MPTP regimens (single MPTP regimen: 2 mg/kg, s.c., per day for 3 consecutive days) were used for PET measurement and behavioral observation. To measure immobility as a central PD sign, locomotion of marmosets in their individual living cages were recorded daily by infrared sensors. Daily locomotion counts decreased drastically after MPTP regimens and remained diminished for several months or more. PET scan of the brain, using [(11)C]PE2I as a ligand of the dopamine (DA) transporter, was performed once several months after the last MPTP regimen. The mean binding potential (BP(ND)) in the striatum (putamen and caudate) of the MPTP-marmoset group was significantly lower than that of the MPTP-free control group (n = 5 for each group). In the MPTP-marmosets, the decrease of BP(ND) in the striatum closely correlated with the decrease in locomotion counts (r = 0.98 in putamen and 0.91 in caudate). CONCLUSION/SIGNIFICANCE: The present characterization of neural degeneration using non-invasive PET imaging and of behavioral manifestation in the MPTP marmoset mimics typical PD characteristics and can be useful in evaluating the phenotype of TG marmosets being developed. |
format | Online Article Text |
id | pubmed-3466292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34662922012-10-10 PET Analysis of Dopaminergic Neurodegeneration in Relation to Immobility in the MPTP-Treated Common Marmoset, a Model for Parkinson’s Disease Ando, Kiyoshi Obayashi, Shigeru Nagai, Yuji Oh-Nishi, Arata Minamimoto, Takafumi Higuchi, Makoto Inoue, Takashi Itoh, Toshio Suhara, Tetsuya PLoS One Research Article BACKGROUND: Positron Emission Tomography (PET) measurement was applied to the brain of the common marmoset, a small primate species, treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). The marmoset shows prominent Parkinson’s disease (PD) signs due to dopaminergic neural degeneration. Recently, the transgenic marmoset (TG) carrying human PD genes is developing. For phenotypic evaluations of TG, non-invasive PET measurement is considered to be substantially significant. As a reference control for TG, the brain of the MPTP-marmoset as an established and valid model was scanned by PET. Behavioral analysis was also performed by recording locomotion of the MPTP-marmoset, as an objective measure of PD signs. METHODOLOGY/PRINCIPAL FINDINGS: Marmosets received several MPTP regimens (single MPTP regimen: 2 mg/kg, s.c., per day for 3 consecutive days) were used for PET measurement and behavioral observation. To measure immobility as a central PD sign, locomotion of marmosets in their individual living cages were recorded daily by infrared sensors. Daily locomotion counts decreased drastically after MPTP regimens and remained diminished for several months or more. PET scan of the brain, using [(11)C]PE2I as a ligand of the dopamine (DA) transporter, was performed once several months after the last MPTP regimen. The mean binding potential (BP(ND)) in the striatum (putamen and caudate) of the MPTP-marmoset group was significantly lower than that of the MPTP-free control group (n = 5 for each group). In the MPTP-marmosets, the decrease of BP(ND) in the striatum closely correlated with the decrease in locomotion counts (r = 0.98 in putamen and 0.91 in caudate). CONCLUSION/SIGNIFICANCE: The present characterization of neural degeneration using non-invasive PET imaging and of behavioral manifestation in the MPTP marmoset mimics typical PD characteristics and can be useful in evaluating the phenotype of TG marmosets being developed. Public Library of Science 2012-10-08 /pmc/articles/PMC3466292/ /pubmed/23056291 http://dx.doi.org/10.1371/journal.pone.0046371 Text en © 2012 Ando et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ando, Kiyoshi Obayashi, Shigeru Nagai, Yuji Oh-Nishi, Arata Minamimoto, Takafumi Higuchi, Makoto Inoue, Takashi Itoh, Toshio Suhara, Tetsuya PET Analysis of Dopaminergic Neurodegeneration in Relation to Immobility in the MPTP-Treated Common Marmoset, a Model for Parkinson’s Disease |
title | PET Analysis of Dopaminergic Neurodegeneration in Relation to Immobility in the MPTP-Treated Common Marmoset, a Model for Parkinson’s Disease |
title_full | PET Analysis of Dopaminergic Neurodegeneration in Relation to Immobility in the MPTP-Treated Common Marmoset, a Model for Parkinson’s Disease |
title_fullStr | PET Analysis of Dopaminergic Neurodegeneration in Relation to Immobility in the MPTP-Treated Common Marmoset, a Model for Parkinson’s Disease |
title_full_unstemmed | PET Analysis of Dopaminergic Neurodegeneration in Relation to Immobility in the MPTP-Treated Common Marmoset, a Model for Parkinson’s Disease |
title_short | PET Analysis of Dopaminergic Neurodegeneration in Relation to Immobility in the MPTP-Treated Common Marmoset, a Model for Parkinson’s Disease |
title_sort | pet analysis of dopaminergic neurodegeneration in relation to immobility in the mptp-treated common marmoset, a model for parkinson’s disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466292/ https://www.ncbi.nlm.nih.gov/pubmed/23056291 http://dx.doi.org/10.1371/journal.pone.0046371 |
work_keys_str_mv | AT andokiyoshi petanalysisofdopaminergicneurodegenerationinrelationtoimmobilityinthemptptreatedcommonmarmosetamodelforparkinsonsdisease AT obayashishigeru petanalysisofdopaminergicneurodegenerationinrelationtoimmobilityinthemptptreatedcommonmarmosetamodelforparkinsonsdisease AT nagaiyuji petanalysisofdopaminergicneurodegenerationinrelationtoimmobilityinthemptptreatedcommonmarmosetamodelforparkinsonsdisease AT ohnishiarata petanalysisofdopaminergicneurodegenerationinrelationtoimmobilityinthemptptreatedcommonmarmosetamodelforparkinsonsdisease AT minamimototakafumi petanalysisofdopaminergicneurodegenerationinrelationtoimmobilityinthemptptreatedcommonmarmosetamodelforparkinsonsdisease AT higuchimakoto petanalysisofdopaminergicneurodegenerationinrelationtoimmobilityinthemptptreatedcommonmarmosetamodelforparkinsonsdisease AT inouetakashi petanalysisofdopaminergicneurodegenerationinrelationtoimmobilityinthemptptreatedcommonmarmosetamodelforparkinsonsdisease AT itohtoshio petanalysisofdopaminergicneurodegenerationinrelationtoimmobilityinthemptptreatedcommonmarmosetamodelforparkinsonsdisease AT suharatetsuya petanalysisofdopaminergicneurodegenerationinrelationtoimmobilityinthemptptreatedcommonmarmosetamodelforparkinsonsdisease |