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Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma

BACKGROUND: Elevated serum alpha-fetoprotein (AFP) is not only a diagnostic marker for hepatocellular carcinoma (HCC), but is also a risk factor for HCC in chronic hepatitis C patients who do not have HCC. AIM: The aim was to analyse the hepatic gene expression signature in chronic hepatitis C patie...

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Autores principales: Sato, Shunsuke, Genda, Takuya, Hirano, Katsuharu, Tsuzura, Hironori, Narita, Yutaka, Kanemitsu, Yoshio, Kikuchi, Tetsu, Iijima, Katsuyori, Wada, Ryo, Ichida, Takafumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466415/
https://www.ncbi.nlm.nih.gov/pubmed/22681639
http://dx.doi.org/10.1111/j.1478-3231.2012.02827.x
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author Sato, Shunsuke
Genda, Takuya
Hirano, Katsuharu
Tsuzura, Hironori
Narita, Yutaka
Kanemitsu, Yoshio
Kikuchi, Tetsu
Iijima, Katsuyori
Wada, Ryo
Ichida, Takafumi
author_facet Sato, Shunsuke
Genda, Takuya
Hirano, Katsuharu
Tsuzura, Hironori
Narita, Yutaka
Kanemitsu, Yoshio
Kikuchi, Tetsu
Iijima, Katsuyori
Wada, Ryo
Ichida, Takafumi
author_sort Sato, Shunsuke
collection PubMed
description BACKGROUND: Elevated serum alpha-fetoprotein (AFP) is not only a diagnostic marker for hepatocellular carcinoma (HCC), but is also a risk factor for HCC in chronic hepatitis C patients who do not have HCC. AIM: The aim was to analyse the hepatic gene expression signature in chronic hepatitis C patients with elevated AFP, who were at high risk for HCC. METHODS: Liver tissue samples from 48 chronic hepatitis C patients were stratified by their serum AFP levels and analysed for gene expression profiles. The association between aldo-keto reductase family 1 member B10 (AKR1B10) expression and serum AFP was confirmed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemical analyses. A matched case-control study was performed to evaluate the risk of AKR1B10 expression for HCC development. RESULTS: Distinct hepatic gene expression patterns were demonstrated in patients with elevated AFP (≥10 ng/mL) and normal AFP (<10 ng/mL). Of the 627 differently expressed genes, the most abundantly expressed gene in patients with elevated AFP was AKR1B10 (fold change, 26.2; P < 0.001), which was originally isolated as an overexpressed gene in human HCC. The qRT-PCR and immunohistochemical studies confirmed a proportional correlation between AKR1B10 expression and serum AFP. A matched case-control study identified that AKR1B10 up-regulation (>6%) was an independent risk factor for HCC development (hazard ratio, 21.4; P = 0.001). CONCLUSION: AKR1B10 was up-regulated in association with serum AFP, and was an independent risk factor for HCC in chronic hepatitis C patients, suggesting its possible involvement at a very early stage of hepatocarcinogenesis.
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spelling pubmed-34664152012-10-11 Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma Sato, Shunsuke Genda, Takuya Hirano, Katsuharu Tsuzura, Hironori Narita, Yutaka Kanemitsu, Yoshio Kikuchi, Tetsu Iijima, Katsuyori Wada, Ryo Ichida, Takafumi Liver Int Clinical Studies BACKGROUND: Elevated serum alpha-fetoprotein (AFP) is not only a diagnostic marker for hepatocellular carcinoma (HCC), but is also a risk factor for HCC in chronic hepatitis C patients who do not have HCC. AIM: The aim was to analyse the hepatic gene expression signature in chronic hepatitis C patients with elevated AFP, who were at high risk for HCC. METHODS: Liver tissue samples from 48 chronic hepatitis C patients were stratified by their serum AFP levels and analysed for gene expression profiles. The association between aldo-keto reductase family 1 member B10 (AKR1B10) expression and serum AFP was confirmed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemical analyses. A matched case-control study was performed to evaluate the risk of AKR1B10 expression for HCC development. RESULTS: Distinct hepatic gene expression patterns were demonstrated in patients with elevated AFP (≥10 ng/mL) and normal AFP (<10 ng/mL). Of the 627 differently expressed genes, the most abundantly expressed gene in patients with elevated AFP was AKR1B10 (fold change, 26.2; P < 0.001), which was originally isolated as an overexpressed gene in human HCC. The qRT-PCR and immunohistochemical studies confirmed a proportional correlation between AKR1B10 expression and serum AFP. A matched case-control study identified that AKR1B10 up-regulation (>6%) was an independent risk factor for HCC development (hazard ratio, 21.4; P = 0.001). CONCLUSION: AKR1B10 was up-regulated in association with serum AFP, and was an independent risk factor for HCC in chronic hepatitis C patients, suggesting its possible involvement at a very early stage of hepatocarcinogenesis. Blackwell Publishing Ltd 2012-10 2012-06-11 /pmc/articles/PMC3466415/ /pubmed/22681639 http://dx.doi.org/10.1111/j.1478-3231.2012.02827.x Text en © 2012 John Wiley & Sons A/S http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Clinical Studies
Sato, Shunsuke
Genda, Takuya
Hirano, Katsuharu
Tsuzura, Hironori
Narita, Yutaka
Kanemitsu, Yoshio
Kikuchi, Tetsu
Iijima, Katsuyori
Wada, Ryo
Ichida, Takafumi
Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma
title Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma
title_full Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma
title_fullStr Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma
title_full_unstemmed Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma
title_short Up-regulated aldo-keto reductase family 1 member B10 in chronic hepatitis C: association with serum alpha-fetoprotein and hepatocellular carcinoma
title_sort up-regulated aldo-keto reductase family 1 member b10 in chronic hepatitis c: association with serum alpha-fetoprotein and hepatocellular carcinoma
topic Clinical Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466415/
https://www.ncbi.nlm.nih.gov/pubmed/22681639
http://dx.doi.org/10.1111/j.1478-3231.2012.02827.x
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