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Silkworms Can be Used as an Animal Model to Screen and Evaluate Gouty Therapeutic Drugs

In the past few decades, the mouse has been used as a mammalian model for hyperuricemia and gout, which has increased not only in prevalence, but also in clinical complexity, accentuated in part by a dearth of novel advances in treatments for hyperuricemia and gouty arthritis. However, the use of mi...

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Autores principales: Zhang, Xiaoli, Xue, Renyu, Cao, Guangli, Pan, Zhonghua, Zheng, Xiaojian, Gong, Chengliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: University of Wisconsin Library 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3467090/
https://www.ncbi.nlm.nih.gov/pubmed/22934965
http://dx.doi.org/10.1673/031.012.0401
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author Zhang, Xiaoli
Xue, Renyu
Cao, Guangli
Pan, Zhonghua
Zheng, Xiaojian
Gong, Chengliang
author_facet Zhang, Xiaoli
Xue, Renyu
Cao, Guangli
Pan, Zhonghua
Zheng, Xiaojian
Gong, Chengliang
author_sort Zhang, Xiaoli
collection PubMed
description In the past few decades, the mouse has been used as a mammalian model for hyperuricemia and gout, which has increased not only in prevalence, but also in clinical complexity, accentuated in part by a dearth of novel advances in treatments for hyperuricemia and gouty arthritis. However, the use of mice for the development of gouty therapeutic drugs creates a number of problems. Thus, identification and evaluation of the therapeutic effects of chemicals in an alternative animal model is desirable. In the present study, the effects of gouty therapeutic drugs on lowering the content of uric acid and inhibiting activity of xanthine oxidase were evaluated by using a silkworm model, Bombyx mori L. (Lepidoptera: Bombycidae). The results showed that the effectiveness of oral administration of various gouty therapeutic drugs to 5(th) instar silkworms is consistent with results for human. The activity of xanthine oxidase of silkworm treated with allopurinol was lower, and declined in a dose-dependent manner compared with control silkworms, while sodium bicarbonate failed at inhibiting the activity of xanthine oxidase. The concentration of uric acid in the both hemolymph and fat body declined by 90 and 95% at six days post-administration with 25 mg/mL of allopurinol, respectively (p < 0.01), while the concentration of uric acid in both the hemolymph and fat body also declined by 81 and 95% at six days post-administration with 25 mg/mL of sodium bicarbonate, respectively (p < 0.01). Moreover, the epidermis of silkworm treated with allopurinol or sodium bicarbonate became transparent compared with the negative control group. These results suggest that silkworm larva can be used as an animal model for screening and evaluation of gouty therapeutic drugs.
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spelling pubmed-34670902012-10-16 Silkworms Can be Used as an Animal Model to Screen and Evaluate Gouty Therapeutic Drugs Zhang, Xiaoli Xue, Renyu Cao, Guangli Pan, Zhonghua Zheng, Xiaojian Gong, Chengliang J Insect Sci Article In the past few decades, the mouse has been used as a mammalian model for hyperuricemia and gout, which has increased not only in prevalence, but also in clinical complexity, accentuated in part by a dearth of novel advances in treatments for hyperuricemia and gouty arthritis. However, the use of mice for the development of gouty therapeutic drugs creates a number of problems. Thus, identification and evaluation of the therapeutic effects of chemicals in an alternative animal model is desirable. In the present study, the effects of gouty therapeutic drugs on lowering the content of uric acid and inhibiting activity of xanthine oxidase were evaluated by using a silkworm model, Bombyx mori L. (Lepidoptera: Bombycidae). The results showed that the effectiveness of oral administration of various gouty therapeutic drugs to 5(th) instar silkworms is consistent with results for human. The activity of xanthine oxidase of silkworm treated with allopurinol was lower, and declined in a dose-dependent manner compared with control silkworms, while sodium bicarbonate failed at inhibiting the activity of xanthine oxidase. The concentration of uric acid in the both hemolymph and fat body declined by 90 and 95% at six days post-administration with 25 mg/mL of allopurinol, respectively (p < 0.01), while the concentration of uric acid in both the hemolymph and fat body also declined by 81 and 95% at six days post-administration with 25 mg/mL of sodium bicarbonate, respectively (p < 0.01). Moreover, the epidermis of silkworm treated with allopurinol or sodium bicarbonate became transparent compared with the negative control group. These results suggest that silkworm larva can be used as an animal model for screening and evaluation of gouty therapeutic drugs. University of Wisconsin Library 2012-01-22 /pmc/articles/PMC3467090/ /pubmed/22934965 http://dx.doi.org/10.1673/031.012.0401 Text en © 2012 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Zhang, Xiaoli
Xue, Renyu
Cao, Guangli
Pan, Zhonghua
Zheng, Xiaojian
Gong, Chengliang
Silkworms Can be Used as an Animal Model to Screen and Evaluate Gouty Therapeutic Drugs
title Silkworms Can be Used as an Animal Model to Screen and Evaluate Gouty Therapeutic Drugs
title_full Silkworms Can be Used as an Animal Model to Screen and Evaluate Gouty Therapeutic Drugs
title_fullStr Silkworms Can be Used as an Animal Model to Screen and Evaluate Gouty Therapeutic Drugs
title_full_unstemmed Silkworms Can be Used as an Animal Model to Screen and Evaluate Gouty Therapeutic Drugs
title_short Silkworms Can be Used as an Animal Model to Screen and Evaluate Gouty Therapeutic Drugs
title_sort silkworms can be used as an animal model to screen and evaluate gouty therapeutic drugs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3467090/
https://www.ncbi.nlm.nih.gov/pubmed/22934965
http://dx.doi.org/10.1673/031.012.0401
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