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Time-lapse imaging of the initiation of pollen embryogenesis in barley (Hordeum vulgare L.)

Pollen embryogenesis provides exciting opportunities in the areas of breeding and biotechnology as well as representing a convenient model for studying the process of plant cell proliferation in general and embryogenesis in particular. A cell culture system was devised in which immature barley polle...

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Detalles Bibliográficos
Autores principales: Daghma, D. S., Kumlehn, J., Hensel, G., Rutten, T., Melzer, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3467303/
https://www.ncbi.nlm.nih.gov/pubmed/22991158
http://dx.doi.org/10.1093/jxb/ers254
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author Daghma, D. S.
Kumlehn, J.
Hensel, G.
Rutten, T.
Melzer, M.
author_facet Daghma, D. S.
Kumlehn, J.
Hensel, G.
Rutten, T.
Melzer, M.
author_sort Daghma, D. S.
collection PubMed
description Pollen embryogenesis provides exciting opportunities in the areas of breeding and biotechnology as well as representing a convenient model for studying the process of plant cell proliferation in general and embryogenesis in particular. A cell culture system was devised in which immature barley pollen could be cultured as a monolayer trapped between the bottom glass-cover slip of a live-cell chamber and a diaphanous PTFE membrane within a liquid medium over a period of up to 28 d, allowing the process of embryogenesis to be tracked in individual pollen. Z-stacks of images were automatically captured every 3min, starting from the unicellular pollen stage up to the development of multicellular, embryogenic structures. The method should prove useful for the elucidation of ultrastructural features and molecular processes associated with pollen embryogenesis.
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spelling pubmed-34673032012-10-10 Time-lapse imaging of the initiation of pollen embryogenesis in barley (Hordeum vulgare L.) Daghma, D. S. Kumlehn, J. Hensel, G. Rutten, T. Melzer, M. J Exp Bot Research Paper Pollen embryogenesis provides exciting opportunities in the areas of breeding and biotechnology as well as representing a convenient model for studying the process of plant cell proliferation in general and embryogenesis in particular. A cell culture system was devised in which immature barley pollen could be cultured as a monolayer trapped between the bottom glass-cover slip of a live-cell chamber and a diaphanous PTFE membrane within a liquid medium over a period of up to 28 d, allowing the process of embryogenesis to be tracked in individual pollen. Z-stacks of images were automatically captured every 3min, starting from the unicellular pollen stage up to the development of multicellular, embryogenic structures. The method should prove useful for the elucidation of ultrastructural features and molecular processes associated with pollen embryogenesis. Oxford University Press 2012-10 2012-09-24 /pmc/articles/PMC3467303/ /pubmed/22991158 http://dx.doi.org/10.1093/jxb/ers254 Text en © 2012 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Daghma, D. S.
Kumlehn, J.
Hensel, G.
Rutten, T.
Melzer, M.
Time-lapse imaging of the initiation of pollen embryogenesis in barley (Hordeum vulgare L.)
title Time-lapse imaging of the initiation of pollen embryogenesis in barley (Hordeum vulgare L.)
title_full Time-lapse imaging of the initiation of pollen embryogenesis in barley (Hordeum vulgare L.)
title_fullStr Time-lapse imaging of the initiation of pollen embryogenesis in barley (Hordeum vulgare L.)
title_full_unstemmed Time-lapse imaging of the initiation of pollen embryogenesis in barley (Hordeum vulgare L.)
title_short Time-lapse imaging of the initiation of pollen embryogenesis in barley (Hordeum vulgare L.)
title_sort time-lapse imaging of the initiation of pollen embryogenesis in barley (hordeum vulgare l.)
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3467303/
https://www.ncbi.nlm.nih.gov/pubmed/22991158
http://dx.doi.org/10.1093/jxb/ers254
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