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Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times
The aim of this study was to assess changes of Hsp70 and HSF-1 protein and mRNA expression in stress-sensitive organs of pigs during transportation for various periods of time. Twenty pigs were randomly divided into four groups (0 h, 1 h, 2 h, and 4 h of transportation). A significant increased acti...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3467400/ https://www.ncbi.nlm.nih.gov/pubmed/23000582 http://dx.doi.org/10.4142/jvs.2012.13.3.253 |
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author | Zhang, Miao Yue, Zhenhua Liu, Zhijun Islam, Ali Rehana, Buriro Tang, Shu Bao, Endong Hartung, Jörg |
author_facet | Zhang, Miao Yue, Zhenhua Liu, Zhijun Islam, Ali Rehana, Buriro Tang, Shu Bao, Endong Hartung, Jörg |
author_sort | Zhang, Miao |
collection | PubMed |
description | The aim of this study was to assess changes of Hsp70 and HSF-1 protein and mRNA expression in stress-sensitive organs of pigs during transportation for various periods of time. Twenty pigs were randomly divided into four groups (0 h, 1 h, 2 h, and 4 h of transportation). A significant increased activity of AST and CK was observed after 1 h and 2 h of transportation. Histopathological changes in the heart, liver, and stomach indicated that these organs sustained different degrees of injury. Hsp70 protein expression in the heart and liver of transported pigs did not change significantly while it increased significantly (p < 0.05) in the stomach. Hsp70 mRNA levels decreased significantly (p < 0.05) in the heart after 4 h of transportation. However, mRNA expression increased significantly in the liver after 1 (p < 0.05) and 4 h (p < 0.01) of transportation, and increased significantly in the stomach of the transported pigs after 1, 4 (p < 0.01), and 2 h (p < 0.05). HSF-1 levels were reduced at 1 and 4 h (p < 0.05) only in the hearts of transported pigs. These results indicate that Hsp70 mediates distinct stress-related functions in different tissues during transportation. |
format | Online Article Text |
id | pubmed-3467400 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34674002012-10-22 Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times Zhang, Miao Yue, Zhenhua Liu, Zhijun Islam, Ali Rehana, Buriro Tang, Shu Bao, Endong Hartung, Jörg J Vet Sci Original Article The aim of this study was to assess changes of Hsp70 and HSF-1 protein and mRNA expression in stress-sensitive organs of pigs during transportation for various periods of time. Twenty pigs were randomly divided into four groups (0 h, 1 h, 2 h, and 4 h of transportation). A significant increased activity of AST and CK was observed after 1 h and 2 h of transportation. Histopathological changes in the heart, liver, and stomach indicated that these organs sustained different degrees of injury. Hsp70 protein expression in the heart and liver of transported pigs did not change significantly while it increased significantly (p < 0.05) in the stomach. Hsp70 mRNA levels decreased significantly (p < 0.05) in the heart after 4 h of transportation. However, mRNA expression increased significantly in the liver after 1 (p < 0.05) and 4 h (p < 0.01) of transportation, and increased significantly in the stomach of the transported pigs after 1, 4 (p < 0.01), and 2 h (p < 0.05). HSF-1 levels were reduced at 1 and 4 h (p < 0.05) only in the hearts of transported pigs. These results indicate that Hsp70 mediates distinct stress-related functions in different tissues during transportation. The Korean Society of Veterinary Science 2012-09 2012-09-20 /pmc/articles/PMC3467400/ /pubmed/23000582 http://dx.doi.org/10.4142/jvs.2012.13.3.253 Text en Copyright © 2012 The Korean Society of Veterinary Science http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Zhang, Miao Yue, Zhenhua Liu, Zhijun Islam, Ali Rehana, Buriro Tang, Shu Bao, Endong Hartung, Jörg Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times |
title | Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times |
title_full | Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times |
title_fullStr | Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times |
title_full_unstemmed | Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times |
title_short | Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times |
title_sort | hsp70 and hsf-1 expression is altered in the tissues of pigs transported for various periods of times |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3467400/ https://www.ncbi.nlm.nih.gov/pubmed/23000582 http://dx.doi.org/10.4142/jvs.2012.13.3.253 |
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