Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR

Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed t...

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Detalles Bibliográficos
Autores principales: Luka, Pam Dachung, Ayebazibwe, Chrisostom, Shamaki, David, Mwiine, Frank Norbert, Erume, Joseph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3467409/
https://www.ncbi.nlm.nih.gov/pubmed/23000590
http://dx.doi.org/10.4142/jvs.2012.13.3.323
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author Luka, Pam Dachung
Ayebazibwe, Chrisostom
Shamaki, David
Mwiine, Frank Norbert
Erume, Joseph
author_facet Luka, Pam Dachung
Ayebazibwe, Chrisostom
Shamaki, David
Mwiine, Frank Norbert
Erume, Joseph
author_sort Luka, Pam Dachung
collection PubMed
description Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.
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spelling pubmed-34674092012-10-22 Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR Luka, Pam Dachung Ayebazibwe, Chrisostom Shamaki, David Mwiine, Frank Norbert Erume, Joseph J Vet Sci Short Communication Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives. The Korean Society of Veterinary Science 2012-09 2012-09-20 /pmc/articles/PMC3467409/ /pubmed/23000590 http://dx.doi.org/10.4142/jvs.2012.13.3.323 Text en Copyright © 2012 The Korean Society of Veterinary Science http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Luka, Pam Dachung
Ayebazibwe, Chrisostom
Shamaki, David
Mwiine, Frank Norbert
Erume, Joseph
Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR
title Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR
title_full Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR
title_fullStr Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR
title_full_unstemmed Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR
title_short Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR
title_sort sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription pcr
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3467409/
https://www.ncbi.nlm.nih.gov/pubmed/23000590
http://dx.doi.org/10.4142/jvs.2012.13.3.323
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