Cargando…
Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss
Hwangryun-haedok-tang (HRT) is the common recipe in traditional Asian medicine, and microbial fermentation is used for the conventional methods for processing traditional medicine. We investigated the inhibitory effect of the n-butanol fraction of HRT (HRT-BU) and fHRT (fHRT-BU) on the RANKL-induced...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468024/ https://www.ncbi.nlm.nih.gov/pubmed/23082080 http://dx.doi.org/10.1155/2012/325791 |
_version_ | 1782245912163123200 |
---|---|
author | Shim, Ki-Shuk Kim, Taesoo Ha, Hyunil Cho, Chang-Won Kim, Han Sung Seo, Dong-Hyun Ma, Jin Yeul |
author_facet | Shim, Ki-Shuk Kim, Taesoo Ha, Hyunil Cho, Chang-Won Kim, Han Sung Seo, Dong-Hyun Ma, Jin Yeul |
author_sort | Shim, Ki-Shuk |
collection | PubMed |
description | Hwangryun-haedok-tang (HRT) is the common recipe in traditional Asian medicine, and microbial fermentation is used for the conventional methods for processing traditional medicine. We investigated the inhibitory effect of the n-butanol fraction of HRT (HRT-BU) and fHRT (fHRT-BU) on the RANKL-induced osteoclastogenesis in bone-marrow-derived macrophages. mRNA expression of osteoclastogenesis-related genes were evaluated by real-time QPCR. The activation of signaling pathways was determined by western blot analysis. The marker compounds of HRT-BU and fHRT-BU were analyzed by HPLC. The inhibitory effect of HRT or fHRT on ovariectomy-induced bone loss were evaluated using OVX rats with orally administered HRT, fHRT (300, 1000 mg/kg), or its vehicle for 12 weeks. fHRT-BU significantly inhibited RANKL-induced osteoclastogenesis, and phosphorylation of p38, IKKα/β, and NF-κBp65 compared to HRT-BU. In addition, fHRT-BU also significantly inhibited the mRNA expression of Nfκb2, TNF-α, NFATc1, TRAP, ATPv0d2, and cathepsin K. Furthermore, administration of fHRT had a greater effect on the increase of BMD, and greater improved bone microstructure of the femora than that of HRT in ovariectomy rats. This study demonstrated that bacterial fermentation enhances the inhibitory effect of HRT on osteoclastogenesis and bone loss. These results suggest that fermented HRT might have the beneficial effects on bone disease by inhibiting osteoclastogenesis. |
format | Online Article Text |
id | pubmed-3468024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-34680242012-10-18 Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss Shim, Ki-Shuk Kim, Taesoo Ha, Hyunil Cho, Chang-Won Kim, Han Sung Seo, Dong-Hyun Ma, Jin Yeul Evid Based Complement Alternat Med Research Article Hwangryun-haedok-tang (HRT) is the common recipe in traditional Asian medicine, and microbial fermentation is used for the conventional methods for processing traditional medicine. We investigated the inhibitory effect of the n-butanol fraction of HRT (HRT-BU) and fHRT (fHRT-BU) on the RANKL-induced osteoclastogenesis in bone-marrow-derived macrophages. mRNA expression of osteoclastogenesis-related genes were evaluated by real-time QPCR. The activation of signaling pathways was determined by western blot analysis. The marker compounds of HRT-BU and fHRT-BU were analyzed by HPLC. The inhibitory effect of HRT or fHRT on ovariectomy-induced bone loss were evaluated using OVX rats with orally administered HRT, fHRT (300, 1000 mg/kg), or its vehicle for 12 weeks. fHRT-BU significantly inhibited RANKL-induced osteoclastogenesis, and phosphorylation of p38, IKKα/β, and NF-κBp65 compared to HRT-BU. In addition, fHRT-BU also significantly inhibited the mRNA expression of Nfκb2, TNF-α, NFATc1, TRAP, ATPv0d2, and cathepsin K. Furthermore, administration of fHRT had a greater effect on the increase of BMD, and greater improved bone microstructure of the femora than that of HRT in ovariectomy rats. This study demonstrated that bacterial fermentation enhances the inhibitory effect of HRT on osteoclastogenesis and bone loss. These results suggest that fermented HRT might have the beneficial effects on bone disease by inhibiting osteoclastogenesis. Hindawi Publishing Corporation 2012 2012-10-02 /pmc/articles/PMC3468024/ /pubmed/23082080 http://dx.doi.org/10.1155/2012/325791 Text en Copyright © 2012 Ki-Shuk Shim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Shim, Ki-Shuk Kim, Taesoo Ha, Hyunil Cho, Chang-Won Kim, Han Sung Seo, Dong-Hyun Ma, Jin Yeul Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss |
title | Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss |
title_full | Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss |
title_fullStr | Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss |
title_full_unstemmed | Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss |
title_short | Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss |
title_sort | hwangryun-haedok-tang fermented with lactobacillus casei suppresses ovariectomy-induced bone loss |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468024/ https://www.ncbi.nlm.nih.gov/pubmed/23082080 http://dx.doi.org/10.1155/2012/325791 |
work_keys_str_mv | AT shimkishuk hwangryunhaedoktangfermentedwithlactobacilluscaseisuppressesovariectomyinducedboneloss AT kimtaesoo hwangryunhaedoktangfermentedwithlactobacilluscaseisuppressesovariectomyinducedboneloss AT hahyunil hwangryunhaedoktangfermentedwithlactobacilluscaseisuppressesovariectomyinducedboneloss AT chochangwon hwangryunhaedoktangfermentedwithlactobacilluscaseisuppressesovariectomyinducedboneloss AT kimhansung hwangryunhaedoktangfermentedwithlactobacilluscaseisuppressesovariectomyinducedboneloss AT seodonghyun hwangryunhaedoktangfermentedwithlactobacilluscaseisuppressesovariectomyinducedboneloss AT majinyeul hwangryunhaedoktangfermentedwithlactobacilluscaseisuppressesovariectomyinducedboneloss |