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Identifying the Main Mosquito Species in China Based on DNA Barcoding

Mosquitoes are insects of the Diptera, Nematocera, and Culicidae families, some species of which are important disease vectors. Identifying mosquito species based on morphological characteristics is difficult, particularly the identification of specimens collected in the field as part of disease sur...

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Autores principales: Wang, Gang, Li, Chunxiao, Guo, Xiaoxia, Xing, Dan, Dong, Yande, Wang, Zhongming, Zhang, Yingmei, Liu, Meide, Zheng, Zhong, Zhang, Hengduan, Zhu, Xiaojuan, Wu, Zhiming, Zhao, Tongyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468562/
https://www.ncbi.nlm.nih.gov/pubmed/23071708
http://dx.doi.org/10.1371/journal.pone.0047051
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author Wang, Gang
Li, Chunxiao
Guo, Xiaoxia
Xing, Dan
Dong, Yande
Wang, Zhongming
Zhang, Yingmei
Liu, Meide
Zheng, Zhong
Zhang, Hengduan
Zhu, Xiaojuan
Wu, Zhiming
Zhao, Tongyan
author_facet Wang, Gang
Li, Chunxiao
Guo, Xiaoxia
Xing, Dan
Dong, Yande
Wang, Zhongming
Zhang, Yingmei
Liu, Meide
Zheng, Zhong
Zhang, Hengduan
Zhu, Xiaojuan
Wu, Zhiming
Zhao, Tongyan
author_sort Wang, Gang
collection PubMed
description Mosquitoes are insects of the Diptera, Nematocera, and Culicidae families, some species of which are important disease vectors. Identifying mosquito species based on morphological characteristics is difficult, particularly the identification of specimens collected in the field as part of disease surveillance programs. Because of this difficulty, we constructed DNA barcodes of the cytochrome c oxidase subunit 1, the COI gene, for the more common mosquito species in China, including the major disease vectors. A total of 404 mosquito specimens were collected and assigned to 15 genera and 122 species and subspecies on the basis of morphological characteristics. Individuals of the same species grouped closely together in a Neighborhood-Joining tree based on COI sequence similarity, regardless of collection site. COI gene sequence divergence was approximately 30 times higher for species in the same genus than for members of the same species. Divergence in over 98% of congeneric species ranged from 2.3% to 21.8%, whereas divergence in conspecific individuals ranged from 0% to 1.67%. Cryptic species may be common and a few pseudogenes were detected.
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spelling pubmed-34685622012-10-15 Identifying the Main Mosquito Species in China Based on DNA Barcoding Wang, Gang Li, Chunxiao Guo, Xiaoxia Xing, Dan Dong, Yande Wang, Zhongming Zhang, Yingmei Liu, Meide Zheng, Zhong Zhang, Hengduan Zhu, Xiaojuan Wu, Zhiming Zhao, Tongyan PLoS One Research Article Mosquitoes are insects of the Diptera, Nematocera, and Culicidae families, some species of which are important disease vectors. Identifying mosquito species based on morphological characteristics is difficult, particularly the identification of specimens collected in the field as part of disease surveillance programs. Because of this difficulty, we constructed DNA barcodes of the cytochrome c oxidase subunit 1, the COI gene, for the more common mosquito species in China, including the major disease vectors. A total of 404 mosquito specimens were collected and assigned to 15 genera and 122 species and subspecies on the basis of morphological characteristics. Individuals of the same species grouped closely together in a Neighborhood-Joining tree based on COI sequence similarity, regardless of collection site. COI gene sequence divergence was approximately 30 times higher for species in the same genus than for members of the same species. Divergence in over 98% of congeneric species ranged from 2.3% to 21.8%, whereas divergence in conspecific individuals ranged from 0% to 1.67%. Cryptic species may be common and a few pseudogenes were detected. Public Library of Science 2012-10-10 /pmc/articles/PMC3468562/ /pubmed/23071708 http://dx.doi.org/10.1371/journal.pone.0047051 Text en © 2012 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Gang
Li, Chunxiao
Guo, Xiaoxia
Xing, Dan
Dong, Yande
Wang, Zhongming
Zhang, Yingmei
Liu, Meide
Zheng, Zhong
Zhang, Hengduan
Zhu, Xiaojuan
Wu, Zhiming
Zhao, Tongyan
Identifying the Main Mosquito Species in China Based on DNA Barcoding
title Identifying the Main Mosquito Species in China Based on DNA Barcoding
title_full Identifying the Main Mosquito Species in China Based on DNA Barcoding
title_fullStr Identifying the Main Mosquito Species in China Based on DNA Barcoding
title_full_unstemmed Identifying the Main Mosquito Species in China Based on DNA Barcoding
title_short Identifying the Main Mosquito Species in China Based on DNA Barcoding
title_sort identifying the main mosquito species in china based on dna barcoding
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468562/
https://www.ncbi.nlm.nih.gov/pubmed/23071708
http://dx.doi.org/10.1371/journal.pone.0047051
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