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Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae

BACKGROUND: Sap sucking hemipteran aphids damage diverse crop species. Although delivery of ds-RNA or siRNA through microinjection/feeding has been demonstrated, the efficacy of host-mediated delivery of aphid-specific dsRNA in developing aphid resistance has been far from being elucidated. METHODOL...

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Autores principales: Bhatia, Varnika, Bhattacharya, Ramcharan, Uniyal, Prem L., Singh, Rajendra, Niranjan, Rampal S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468595/
https://www.ncbi.nlm.nih.gov/pubmed/23071558
http://dx.doi.org/10.1371/journal.pone.0046343
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author Bhatia, Varnika
Bhattacharya, Ramcharan
Uniyal, Prem L.
Singh, Rajendra
Niranjan, Rampal S.
author_facet Bhatia, Varnika
Bhattacharya, Ramcharan
Uniyal, Prem L.
Singh, Rajendra
Niranjan, Rampal S.
author_sort Bhatia, Varnika
collection PubMed
description BACKGROUND: Sap sucking hemipteran aphids damage diverse crop species. Although delivery of ds-RNA or siRNA through microinjection/feeding has been demonstrated, the efficacy of host-mediated delivery of aphid-specific dsRNA in developing aphid resistance has been far from being elucidated. METHODOLOGY/PRINCIPAL FINDINGS: Transgenic Arabidopsis expressing ds-RNA of Myzus persicae serine protease (MySP) was developed that triggered the generation of corresponding siRNAs amenable for delivery to the feeding aphids. M. persicae when fed on the transgenic plants for different time intervals under controlled growth conditions resulted in a significant attenuation of the expression of MySP and a commensurate decline in gut protease activity. Although the survivability of these aphids was not affected, there was a noticeable decline in their fecundity resulting in a significant reduction in parthenogenetic population. CONCLUSIONS/SIGNIFICANCE: The study highlighted the feasibility of developing host based RNAi-mediated resistance against hemipteran pest aphids.
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spelling pubmed-34685952012-10-15 Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae Bhatia, Varnika Bhattacharya, Ramcharan Uniyal, Prem L. Singh, Rajendra Niranjan, Rampal S. PLoS One Research Article BACKGROUND: Sap sucking hemipteran aphids damage diverse crop species. Although delivery of ds-RNA or siRNA through microinjection/feeding has been demonstrated, the efficacy of host-mediated delivery of aphid-specific dsRNA in developing aphid resistance has been far from being elucidated. METHODOLOGY/PRINCIPAL FINDINGS: Transgenic Arabidopsis expressing ds-RNA of Myzus persicae serine protease (MySP) was developed that triggered the generation of corresponding siRNAs amenable for delivery to the feeding aphids. M. persicae when fed on the transgenic plants for different time intervals under controlled growth conditions resulted in a significant attenuation of the expression of MySP and a commensurate decline in gut protease activity. Although the survivability of these aphids was not affected, there was a noticeable decline in their fecundity resulting in a significant reduction in parthenogenetic population. CONCLUSIONS/SIGNIFICANCE: The study highlighted the feasibility of developing host based RNAi-mediated resistance against hemipteran pest aphids. Public Library of Science 2012-10-10 /pmc/articles/PMC3468595/ /pubmed/23071558 http://dx.doi.org/10.1371/journal.pone.0046343 Text en © 2012 Bhatia et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bhatia, Varnika
Bhattacharya, Ramcharan
Uniyal, Prem L.
Singh, Rajendra
Niranjan, Rampal S.
Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
title Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
title_full Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
title_fullStr Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
title_full_unstemmed Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
title_short Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
title_sort host generated sirnas attenuate expression of serine protease gene in myzus persicae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468595/
https://www.ncbi.nlm.nih.gov/pubmed/23071558
http://dx.doi.org/10.1371/journal.pone.0046343
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