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Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
BACKGROUND: Sap sucking hemipteran aphids damage diverse crop species. Although delivery of ds-RNA or siRNA through microinjection/feeding has been demonstrated, the efficacy of host-mediated delivery of aphid-specific dsRNA in developing aphid resistance has been far from being elucidated. METHODOL...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468595/ https://www.ncbi.nlm.nih.gov/pubmed/23071558 http://dx.doi.org/10.1371/journal.pone.0046343 |
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author | Bhatia, Varnika Bhattacharya, Ramcharan Uniyal, Prem L. Singh, Rajendra Niranjan, Rampal S. |
author_facet | Bhatia, Varnika Bhattacharya, Ramcharan Uniyal, Prem L. Singh, Rajendra Niranjan, Rampal S. |
author_sort | Bhatia, Varnika |
collection | PubMed |
description | BACKGROUND: Sap sucking hemipteran aphids damage diverse crop species. Although delivery of ds-RNA or siRNA through microinjection/feeding has been demonstrated, the efficacy of host-mediated delivery of aphid-specific dsRNA in developing aphid resistance has been far from being elucidated. METHODOLOGY/PRINCIPAL FINDINGS: Transgenic Arabidopsis expressing ds-RNA of Myzus persicae serine protease (MySP) was developed that triggered the generation of corresponding siRNAs amenable for delivery to the feeding aphids. M. persicae when fed on the transgenic plants for different time intervals under controlled growth conditions resulted in a significant attenuation of the expression of MySP and a commensurate decline in gut protease activity. Although the survivability of these aphids was not affected, there was a noticeable decline in their fecundity resulting in a significant reduction in parthenogenetic population. CONCLUSIONS/SIGNIFICANCE: The study highlighted the feasibility of developing host based RNAi-mediated resistance against hemipteran pest aphids. |
format | Online Article Text |
id | pubmed-3468595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34685952012-10-15 Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae Bhatia, Varnika Bhattacharya, Ramcharan Uniyal, Prem L. Singh, Rajendra Niranjan, Rampal S. PLoS One Research Article BACKGROUND: Sap sucking hemipteran aphids damage diverse crop species. Although delivery of ds-RNA or siRNA through microinjection/feeding has been demonstrated, the efficacy of host-mediated delivery of aphid-specific dsRNA in developing aphid resistance has been far from being elucidated. METHODOLOGY/PRINCIPAL FINDINGS: Transgenic Arabidopsis expressing ds-RNA of Myzus persicae serine protease (MySP) was developed that triggered the generation of corresponding siRNAs amenable for delivery to the feeding aphids. M. persicae when fed on the transgenic plants for different time intervals under controlled growth conditions resulted in a significant attenuation of the expression of MySP and a commensurate decline in gut protease activity. Although the survivability of these aphids was not affected, there was a noticeable decline in their fecundity resulting in a significant reduction in parthenogenetic population. CONCLUSIONS/SIGNIFICANCE: The study highlighted the feasibility of developing host based RNAi-mediated resistance against hemipteran pest aphids. Public Library of Science 2012-10-10 /pmc/articles/PMC3468595/ /pubmed/23071558 http://dx.doi.org/10.1371/journal.pone.0046343 Text en © 2012 Bhatia et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bhatia, Varnika Bhattacharya, Ramcharan Uniyal, Prem L. Singh, Rajendra Niranjan, Rampal S. Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae |
title | Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
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title_full | Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
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title_fullStr | Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
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title_full_unstemmed | Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
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title_short | Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
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title_sort | host generated sirnas attenuate expression of serine protease gene in myzus persicae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3468595/ https://www.ncbi.nlm.nih.gov/pubmed/23071558 http://dx.doi.org/10.1371/journal.pone.0046343 |
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