In Vitro Effect of Antigenic Extract of Trichophyton verrucosum on Fibroblast Proliferation and Matrix Metalloproteinase -2 Activities

BACKGROUND: Trichophyton verrucosum is a zoophilic fungus with a worldwide distribution. Our aim was to investigate the proliferative effect of antigenic compounds of T. verrucosum on dermis fibro blasts and endothelial cells. METHODS: T. verrucosum was cultured in SCC medium and was then transferred to a broth medium. Surface antigens of this fungus were separated using the freeze and thaw method. The sample was centrifuged and the supernatant was taken. The supernatant was homogenized and purified. The prepared antigenic extract was added to fibro blast cell lines according to a regular timetable. Cytotoxicity and cell proliferation were evaluated using zymography and densitometry in order to assay MMPs activity. RESULTS: Statistical analyses showed that this antigenic extract is able to enhance the MMPs activity CONCLUSION: Trichophyton verrucosum increases the proliferation of dermis germinal layer and MMP-2 activity, which has a direct relation with wound healing process.