Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran

BACKGROUND: The aim of this study was to apply the nested-PCR and bioassay methods in detection and genotyping of Toxoplasma gondii infection in provided sheep aborted fetus samples from Qazvin Province of Iran. METHODS: Eighteen sheep aborted fetal samples were studied by nested-PCR-RFLP, histopath...

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Autores principales: Habibi, GR, Imani, AR, Gholami, MR, Hablolvarid, MH, Behroozikhah, AM, Lotfi, M, Kamalzade, M, Najjar, E, Esmaeil-Nia, K, Bozorgi, S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2012
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469174/
https://www.ncbi.nlm.nih.gov/pubmed/23109964
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author Habibi, GR
Imani, AR
Gholami, MR
Hablolvarid, MH
Behroozikhah, AM
Lotfi, M
Kamalzade, M
Najjar, E
Esmaeil-Nia, K
Bozorgi, S
author_facet Habibi, GR
Imani, AR
Gholami, MR
Hablolvarid, MH
Behroozikhah, AM
Lotfi, M
Kamalzade, M
Najjar, E
Esmaeil-Nia, K
Bozorgi, S
author_sort Habibi, GR
collection PubMed
description BACKGROUND: The aim of this study was to apply the nested-PCR and bioassay methods in detection and genotyping of Toxoplasma gondii infection in provided sheep aborted fetus samples from Qazvin Province of Iran. METHODS: Eighteen sheep aborted fetal samples were studied by nested-PCR-RFLP, histopathological observation and microbiological assay. Bioassay in mice was carried out by inoculating the brain samples intraperitoneally. RESULTS: The results demonstrated the frequency of 66% infected sheep aborted fetal samples with T. gondii type one. Although we could not isolate any parasite from inoculated mice even after three passages, but it was confirmed histopathologically formation of cyst like bodies in prepared mice brain sections. CONCLUSION: The results of the performed nested-PCR and formation of brain cyst in inoculated mice exhibited that T. gondii type one infection might be considered as one of the major causative agents for abortion in ewes.
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spelling pubmed-34691742012-10-29 Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran Habibi, GR Imani, AR Gholami, MR Hablolvarid, MH Behroozikhah, AM Lotfi, M Kamalzade, M Najjar, E Esmaeil-Nia, K Bozorgi, S Iran J Parasitol Original Article BACKGROUND: The aim of this study was to apply the nested-PCR and bioassay methods in detection and genotyping of Toxoplasma gondii infection in provided sheep aborted fetus samples from Qazvin Province of Iran. METHODS: Eighteen sheep aborted fetal samples were studied by nested-PCR-RFLP, histopathological observation and microbiological assay. Bioassay in mice was carried out by inoculating the brain samples intraperitoneally. RESULTS: The results demonstrated the frequency of 66% infected sheep aborted fetal samples with T. gondii type one. Although we could not isolate any parasite from inoculated mice even after three passages, but it was confirmed histopathologically formation of cyst like bodies in prepared mice brain sections. CONCLUSION: The results of the performed nested-PCR and formation of brain cyst in inoculated mice exhibited that T. gondii type one infection might be considered as one of the major causative agents for abortion in ewes. Tehran University of Medical Sciences 2012 /pmc/articles/PMC3469174/ /pubmed/23109964 Text en © 2012 Iranian Society of Parasitology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Habibi, GR
Imani, AR
Gholami, MR
Hablolvarid, MH
Behroozikhah, AM
Lotfi, M
Kamalzade, M
Najjar, E
Esmaeil-Nia, K
Bozorgi, S
Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran
title Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran
title_full Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran
title_fullStr Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran
title_full_unstemmed Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran
title_short Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran
title_sort detection and identification of toxoplasma gondii type one infection in sheep aborted fetuses in qazvin province of iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469174/
https://www.ncbi.nlm.nih.gov/pubmed/23109964
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