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Bio-Rad’s Bio-Plex® suspension array system, xMAP technology overview
The Bio-Plex® system utilizes xMAP technology to permit the multiplexing of up to 100 different analytes. Multiplex analysis gives researchers the ability to look at analytes simultaneously providing more information from less sample volume in less time than traditional immunoassay methods. Similar...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Informa Healthcare
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469222/ https://www.ncbi.nlm.nih.gov/pubmed/22852821 http://dx.doi.org/10.3109/13813455.2012.705301 |
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author | Houser, Brett |
author_facet | Houser, Brett |
author_sort | Houser, Brett |
collection | PubMed |
description | The Bio-Plex® system utilizes xMAP technology to permit the multiplexing of up to 100 different analytes. Multiplex analysis gives researchers the ability to look at analytes simultaneously providing more information from less sample volume in less time than traditional immunoassay methods. Similar to ELISA, xMAP utilizes an antibody sandwich for detection but differs from ELISA in capture substrate and detection method. Rather than a flat surface, Bio-Plex®assays make use of differentially detectable bead sets as a substrate capturing analytes in solution and employs fluorescent methods for detection. These bead sets identify the analytes and detection antibodies are used to measure the quantity of analyte. The use of differentially detectable beads enables the simultaneous identification and quantification of many analytes in the same sample. |
format | Online Article Text |
id | pubmed-3469222 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Informa Healthcare |
record_format | MEDLINE/PubMed |
spelling | pubmed-34692222012-10-15 Bio-Rad’s Bio-Plex® suspension array system, xMAP technology overview Houser, Brett Arch Physiol Biochem Original Article The Bio-Plex® system utilizes xMAP technology to permit the multiplexing of up to 100 different analytes. Multiplex analysis gives researchers the ability to look at analytes simultaneously providing more information from less sample volume in less time than traditional immunoassay methods. Similar to ELISA, xMAP utilizes an antibody sandwich for detection but differs from ELISA in capture substrate and detection method. Rather than a flat surface, Bio-Plex®assays make use of differentially detectable bead sets as a substrate capturing analytes in solution and employs fluorescent methods for detection. These bead sets identify the analytes and detection antibodies are used to measure the quantity of analyte. The use of differentially detectable beads enables the simultaneous identification and quantification of many analytes in the same sample. Informa Healthcare 2012-10 2012-08-02 /pmc/articles/PMC3469222/ /pubmed/22852821 http://dx.doi.org/10.3109/13813455.2012.705301 Text en © 2012 Informa UK Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Informa Healthcare journals (http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Houser, Brett Bio-Rad’s Bio-Plex® suspension array system, xMAP technology overview |
title | Bio-Rad’s Bio-Plex® suspension array system, xMAP technology overview |
title_full | Bio-Rad’s Bio-Plex® suspension array system, xMAP technology overview |
title_fullStr | Bio-Rad’s Bio-Plex® suspension array system, xMAP technology overview |
title_full_unstemmed | Bio-Rad’s Bio-Plex® suspension array system, xMAP technology overview |
title_short | Bio-Rad’s Bio-Plex® suspension array system, xMAP technology overview |
title_sort | bio-rad’s bio-plex® suspension array system, xmap technology overview |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469222/ https://www.ncbi.nlm.nih.gov/pubmed/22852821 http://dx.doi.org/10.3109/13813455.2012.705301 |
work_keys_str_mv | AT houserbrett bioradsbioplexsuspensionarraysystemxmaptechnologyoverview |