Cargando…

The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease

Chronic obstructive pulmonary disease (COPD) is an inflammatory disorder characterized by incompletely reversible airflow obstruction. Bacterial infection of the lower respiratory tract contributes to approximately 50% of COPD exacerbations. Even during periods of stable lung function, the lung harb...

Descripción completa

Detalles Bibliográficos
Autores principales: Pragman, Alexa A., Kim, Hyeun Bum, Reilly, Cavan S., Wendt, Christine, Isaacson, Richard E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469539/
https://www.ncbi.nlm.nih.gov/pubmed/23071781
http://dx.doi.org/10.1371/journal.pone.0047305
_version_ 1782246106266075136
author Pragman, Alexa A.
Kim, Hyeun Bum
Reilly, Cavan S.
Wendt, Christine
Isaacson, Richard E.
author_facet Pragman, Alexa A.
Kim, Hyeun Bum
Reilly, Cavan S.
Wendt, Christine
Isaacson, Richard E.
author_sort Pragman, Alexa A.
collection PubMed
description Chronic obstructive pulmonary disease (COPD) is an inflammatory disorder characterized by incompletely reversible airflow obstruction. Bacterial infection of the lower respiratory tract contributes to approximately 50% of COPD exacerbations. Even during periods of stable lung function, the lung harbors a community of bacteria, termed the microbiome. The role of the lung microbiome in the pathogenesis of COPD remains unknown. The COPD lung microbiome, like the healthy lung microbiome, appears to reflect microaspiration of oral microflora. Here we describe the COPD lung microbiome of 22 patients with Moderate or Severe COPD compared to 10 healthy control patients. The composition of the lung microbiomes was determined using 454 pyrosequencing of 16S rDNA found in bronchoalveolar lavage fluid. Sequences were analyzed using mothur, Ribosomal Database Project, Fast UniFrac, and Metastats. Our results showed a significant increase in microbial diversity with the development of COPD. The main phyla in all samples were Actinobacteria, Firmicutes, and Proteobacteria. Principal coordinate analyses demonstrated separation of control and COPD samples, but samples did not cluster based on disease severity. However, samples did cluster based on the use of inhaled corticosteroids and inhaled bronchodilators. Metastats analyses demonstrated an increased abundance of several oral bacteria in COPD samples.
format Online
Article
Text
id pubmed-3469539
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-34695392012-10-15 The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease Pragman, Alexa A. Kim, Hyeun Bum Reilly, Cavan S. Wendt, Christine Isaacson, Richard E. PLoS One Research Article Chronic obstructive pulmonary disease (COPD) is an inflammatory disorder characterized by incompletely reversible airflow obstruction. Bacterial infection of the lower respiratory tract contributes to approximately 50% of COPD exacerbations. Even during periods of stable lung function, the lung harbors a community of bacteria, termed the microbiome. The role of the lung microbiome in the pathogenesis of COPD remains unknown. The COPD lung microbiome, like the healthy lung microbiome, appears to reflect microaspiration of oral microflora. Here we describe the COPD lung microbiome of 22 patients with Moderate or Severe COPD compared to 10 healthy control patients. The composition of the lung microbiomes was determined using 454 pyrosequencing of 16S rDNA found in bronchoalveolar lavage fluid. Sequences were analyzed using mothur, Ribosomal Database Project, Fast UniFrac, and Metastats. Our results showed a significant increase in microbial diversity with the development of COPD. The main phyla in all samples were Actinobacteria, Firmicutes, and Proteobacteria. Principal coordinate analyses demonstrated separation of control and COPD samples, but samples did not cluster based on disease severity. However, samples did cluster based on the use of inhaled corticosteroids and inhaled bronchodilators. Metastats analyses demonstrated an increased abundance of several oral bacteria in COPD samples. Public Library of Science 2012-10-11 /pmc/articles/PMC3469539/ /pubmed/23071781 http://dx.doi.org/10.1371/journal.pone.0047305 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Pragman, Alexa A.
Kim, Hyeun Bum
Reilly, Cavan S.
Wendt, Christine
Isaacson, Richard E.
The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease
title The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease
title_full The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease
title_fullStr The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease
title_full_unstemmed The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease
title_short The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease
title_sort lung microbiome in moderate and severe chronic obstructive pulmonary disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469539/
https://www.ncbi.nlm.nih.gov/pubmed/23071781
http://dx.doi.org/10.1371/journal.pone.0047305
work_keys_str_mv AT pragmanalexaa thelungmicrobiomeinmoderateandseverechronicobstructivepulmonarydisease
AT kimhyeunbum thelungmicrobiomeinmoderateandseverechronicobstructivepulmonarydisease
AT reillycavans thelungmicrobiomeinmoderateandseverechronicobstructivepulmonarydisease
AT wendtchristine thelungmicrobiomeinmoderateandseverechronicobstructivepulmonarydisease
AT isaacsonricharde thelungmicrobiomeinmoderateandseverechronicobstructivepulmonarydisease
AT pragmanalexaa lungmicrobiomeinmoderateandseverechronicobstructivepulmonarydisease
AT kimhyeunbum lungmicrobiomeinmoderateandseverechronicobstructivepulmonarydisease
AT reillycavans lungmicrobiomeinmoderateandseverechronicobstructivepulmonarydisease
AT wendtchristine lungmicrobiomeinmoderateandseverechronicobstructivepulmonarydisease
AT isaacsonricharde lungmicrobiomeinmoderateandseverechronicobstructivepulmonarydisease