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The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease
Chronic obstructive pulmonary disease (COPD) is an inflammatory disorder characterized by incompletely reversible airflow obstruction. Bacterial infection of the lower respiratory tract contributes to approximately 50% of COPD exacerbations. Even during periods of stable lung function, the lung harb...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469539/ https://www.ncbi.nlm.nih.gov/pubmed/23071781 http://dx.doi.org/10.1371/journal.pone.0047305 |
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author | Pragman, Alexa A. Kim, Hyeun Bum Reilly, Cavan S. Wendt, Christine Isaacson, Richard E. |
author_facet | Pragman, Alexa A. Kim, Hyeun Bum Reilly, Cavan S. Wendt, Christine Isaacson, Richard E. |
author_sort | Pragman, Alexa A. |
collection | PubMed |
description | Chronic obstructive pulmonary disease (COPD) is an inflammatory disorder characterized by incompletely reversible airflow obstruction. Bacterial infection of the lower respiratory tract contributes to approximately 50% of COPD exacerbations. Even during periods of stable lung function, the lung harbors a community of bacteria, termed the microbiome. The role of the lung microbiome in the pathogenesis of COPD remains unknown. The COPD lung microbiome, like the healthy lung microbiome, appears to reflect microaspiration of oral microflora. Here we describe the COPD lung microbiome of 22 patients with Moderate or Severe COPD compared to 10 healthy control patients. The composition of the lung microbiomes was determined using 454 pyrosequencing of 16S rDNA found in bronchoalveolar lavage fluid. Sequences were analyzed using mothur, Ribosomal Database Project, Fast UniFrac, and Metastats. Our results showed a significant increase in microbial diversity with the development of COPD. The main phyla in all samples were Actinobacteria, Firmicutes, and Proteobacteria. Principal coordinate analyses demonstrated separation of control and COPD samples, but samples did not cluster based on disease severity. However, samples did cluster based on the use of inhaled corticosteroids and inhaled bronchodilators. Metastats analyses demonstrated an increased abundance of several oral bacteria in COPD samples. |
format | Online Article Text |
id | pubmed-3469539 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34695392012-10-15 The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease Pragman, Alexa A. Kim, Hyeun Bum Reilly, Cavan S. Wendt, Christine Isaacson, Richard E. PLoS One Research Article Chronic obstructive pulmonary disease (COPD) is an inflammatory disorder characterized by incompletely reversible airflow obstruction. Bacterial infection of the lower respiratory tract contributes to approximately 50% of COPD exacerbations. Even during periods of stable lung function, the lung harbors a community of bacteria, termed the microbiome. The role of the lung microbiome in the pathogenesis of COPD remains unknown. The COPD lung microbiome, like the healthy lung microbiome, appears to reflect microaspiration of oral microflora. Here we describe the COPD lung microbiome of 22 patients with Moderate or Severe COPD compared to 10 healthy control patients. The composition of the lung microbiomes was determined using 454 pyrosequencing of 16S rDNA found in bronchoalveolar lavage fluid. Sequences were analyzed using mothur, Ribosomal Database Project, Fast UniFrac, and Metastats. Our results showed a significant increase in microbial diversity with the development of COPD. The main phyla in all samples were Actinobacteria, Firmicutes, and Proteobacteria. Principal coordinate analyses demonstrated separation of control and COPD samples, but samples did not cluster based on disease severity. However, samples did cluster based on the use of inhaled corticosteroids and inhaled bronchodilators. Metastats analyses demonstrated an increased abundance of several oral bacteria in COPD samples. Public Library of Science 2012-10-11 /pmc/articles/PMC3469539/ /pubmed/23071781 http://dx.doi.org/10.1371/journal.pone.0047305 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Pragman, Alexa A. Kim, Hyeun Bum Reilly, Cavan S. Wendt, Christine Isaacson, Richard E. The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease |
title | The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease |
title_full | The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease |
title_fullStr | The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease |
title_full_unstemmed | The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease |
title_short | The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease |
title_sort | lung microbiome in moderate and severe chronic obstructive pulmonary disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469539/ https://www.ncbi.nlm.nih.gov/pubmed/23071781 http://dx.doi.org/10.1371/journal.pone.0047305 |
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