Optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap

We have integrated a dual-beam optical trap into a microfluidic platform and used it to study membrane mechanics in giant unilamellar vesicles (GUVs). We demonstrate the trapping and stretching of GUVs and characterize the membrane response to a step stress. We then measure area strain as a function...

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Autores principales: Solmaz, Mehmet E., Biswas, Roshni, Sankhagowit, Shalene, Thompson, James R., Mejia, Camilo A., Malmstadt, Noah, Povinelli, Michelle L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Optical Traps, Manipulation, and Tracking
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469987/
https://www.ncbi.nlm.nih.gov/pubmed/23082284
http://dx.doi.org/10.1364/BOE.3.002419
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author Solmaz, Mehmet E.
Biswas, Roshni
Sankhagowit, Shalene
Thompson, James R.
Mejia, Camilo A.
Malmstadt, Noah
Povinelli, Michelle L.
author_facet Solmaz, Mehmet E.
Biswas, Roshni
Sankhagowit, Shalene
Thompson, James R.
Mejia, Camilo A.
Malmstadt, Noah
Povinelli, Michelle L.
author_sort Solmaz, Mehmet E.
collection PubMed
description We have integrated a dual-beam optical trap into a microfluidic platform and used it to study membrane mechanics in giant unilamellar vesicles (GUVs). We demonstrate the trapping and stretching of GUVs and characterize the membrane response to a step stress. We then measure area strain as a function of applied stress to extract the bending modulus of the lipid bilayer in the low-tension regime.
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spelling pubmed-34699872012-10-18 Optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap Solmaz, Mehmet E. Biswas, Roshni Sankhagowit, Shalene Thompson, James R. Mejia, Camilo A. Malmstadt, Noah Povinelli, Michelle L. Biomed Opt Express Optical Traps, Manipulation, and Tracking We have integrated a dual-beam optical trap into a microfluidic platform and used it to study membrane mechanics in giant unilamellar vesicles (GUVs). We demonstrate the trapping and stretching of GUVs and characterize the membrane response to a step stress. We then measure area strain as a function of applied stress to extract the bending modulus of the lipid bilayer in the low-tension regime. Optical Society of America 2012-09-07 /pmc/articles/PMC3469987/ /pubmed/23082284 http://dx.doi.org/10.1364/BOE.3.002419 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Optical Traps, Manipulation, and Tracking
Solmaz, Mehmet E.
Biswas, Roshni
Sankhagowit, Shalene
Thompson, James R.
Mejia, Camilo A.
Malmstadt, Noah
Povinelli, Michelle L.
Optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap
title Optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap
title_full Optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap
title_fullStr Optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap
title_full_unstemmed Optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap
title_short Optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap
title_sort optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap
topic Optical Traps, Manipulation, and Tracking
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469987/
https://www.ncbi.nlm.nih.gov/pubmed/23082284
http://dx.doi.org/10.1364/BOE.3.002419
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