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Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer

Aberrations limit the resolution, signal intensity and achievable imaging depth in microscopy. Coherence-gated wavefront sensing (CGWS) allows the fast measurement of aberrations in scattering samples and therefore the implementation of adaptive corrections. However, CGWS has been demonstrated so fa...

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Autores principales: Wang, Jinyu, Léger, Jean-François, Binding, Jonas, Boccara, A. Claude, Gigan, Sylvain, Bourdieu, Laurent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469991/
https://www.ncbi.nlm.nih.gov/pubmed/23082292
http://dx.doi.org/10.1364/BOE.3.002510
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author Wang, Jinyu
Léger, Jean-François
Binding, Jonas
Boccara, A. Claude
Gigan, Sylvain
Bourdieu, Laurent
author_facet Wang, Jinyu
Léger, Jean-François
Binding, Jonas
Boccara, A. Claude
Gigan, Sylvain
Bourdieu, Laurent
author_sort Wang, Jinyu
collection PubMed
description Aberrations limit the resolution, signal intensity and achievable imaging depth in microscopy. Coherence-gated wavefront sensing (CGWS) allows the fast measurement of aberrations in scattering samples and therefore the implementation of adaptive corrections. However, CGWS has been demonstrated so far only in weakly scattering samples. We designed a new CGWS scheme based on a Linnik interferometer and a SLED light source, which is able to compensate dispersion automatically and can be implemented on any microscope. In the highly scattering rat brain tissue, where multiply scattered photons falling within the temporal gate of the CGWS can no longer be neglected, we have measured known defocus and spherical aberrations up to a depth of 400 µm.
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spelling pubmed-34699912012-10-18 Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer Wang, Jinyu Léger, Jean-François Binding, Jonas Boccara, A. Claude Gigan, Sylvain Bourdieu, Laurent Biomed Opt Express Optics of Tissue and Turbid Media Aberrations limit the resolution, signal intensity and achievable imaging depth in microscopy. Coherence-gated wavefront sensing (CGWS) allows the fast measurement of aberrations in scattering samples and therefore the implementation of adaptive corrections. However, CGWS has been demonstrated so far only in weakly scattering samples. We designed a new CGWS scheme based on a Linnik interferometer and a SLED light source, which is able to compensate dispersion automatically and can be implemented on any microscope. In the highly scattering rat brain tissue, where multiply scattered photons falling within the temporal gate of the CGWS can no longer be neglected, we have measured known defocus and spherical aberrations up to a depth of 400 µm. Optical Society of America 2012-09-13 /pmc/articles/PMC3469991/ /pubmed/23082292 http://dx.doi.org/10.1364/BOE.3.002510 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Optics of Tissue and Turbid Media
Wang, Jinyu
Léger, Jean-François
Binding, Jonas
Boccara, A. Claude
Gigan, Sylvain
Bourdieu, Laurent
Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer
title Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer
title_full Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer
title_fullStr Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer
title_full_unstemmed Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer
title_short Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer
title_sort measuring aberrations in the rat brain by coherence-gated wavefront sensing using a linnik interferometer
topic Optics of Tissue and Turbid Media
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469991/
https://www.ncbi.nlm.nih.gov/pubmed/23082292
http://dx.doi.org/10.1364/BOE.3.002510
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