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Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues

When adipose-derived stem cells (ASCs) are retrieved from the stromal vascular portion of adipose tissue, a large amount of mature adipocytes are often discarded. However, by modified ceiling culture technique based on their buoyancy, mature adipocytes can be easily isolated from the adipose cell su...

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Autores principales: Shen, Jie-fei, Sugawara, Atsunori, Yamashita, Joe, Ogura, Hideo, Sato, Soh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3470092/
https://www.ncbi.nlm.nih.gov/pubmed/21789960
http://dx.doi.org/10.4248/IJOS11044
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author Shen, Jie-fei
Sugawara, Atsunori
Yamashita, Joe
Ogura, Hideo
Sato, Soh
author_facet Shen, Jie-fei
Sugawara, Atsunori
Yamashita, Joe
Ogura, Hideo
Sato, Soh
author_sort Shen, Jie-fei
collection PubMed
description When adipose-derived stem cells (ASCs) are retrieved from the stromal vascular portion of adipose tissue, a large amount of mature adipocytes are often discarded. However, by modified ceiling culture technique based on their buoyancy, mature adipocytes can be easily isolated from the adipose cell suspension and dedifferentiated into lipid-free fibroblast-like cells, named dedifferentiated fat (DFAT) cells. DFAT cells re-establish active proliferation ability and undertake multipotent capacities. Compared with ASCs and other adult stem cells, DFAT cells showed unique advantages in their abundance, isolation and homogeneity. In this concise review, the establishment and culture methods of DFAT cells are introduced and the current profiles of their cellular nature are summarized. Under proper induction culture in vitro or environment in vivo, DFAT cells could demonstrate adipogenic, osteogenic, chondrogenic and myogenic potentials. In angiogenic conditions, DFAT cells could exhibit perivascular characteristics and elicit neovascularization. Our preliminary findings also suggested the pericyte phenotype underlying such cell lineage, which supported a novel interpretation about the common origin of mesenchymal stem cells and tissue-specific stem cells within blood vessel walls. Current research on DFAT cells indicated that this alternative source of adult multipotent cells has great potential in tissue engineering and regenerative medicine.
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spelling pubmed-34700922012-10-12 Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues Shen, Jie-fei Sugawara, Atsunori Yamashita, Joe Ogura, Hideo Sato, Soh Int J Oral Sci Review When adipose-derived stem cells (ASCs) are retrieved from the stromal vascular portion of adipose tissue, a large amount of mature adipocytes are often discarded. However, by modified ceiling culture technique based on their buoyancy, mature adipocytes can be easily isolated from the adipose cell suspension and dedifferentiated into lipid-free fibroblast-like cells, named dedifferentiated fat (DFAT) cells. DFAT cells re-establish active proliferation ability and undertake multipotent capacities. Compared with ASCs and other adult stem cells, DFAT cells showed unique advantages in their abundance, isolation and homogeneity. In this concise review, the establishment and culture methods of DFAT cells are introduced and the current profiles of their cellular nature are summarized. Under proper induction culture in vitro or environment in vivo, DFAT cells could demonstrate adipogenic, osteogenic, chondrogenic and myogenic potentials. In angiogenic conditions, DFAT cells could exhibit perivascular characteristics and elicit neovascularization. Our preliminary findings also suggested the pericyte phenotype underlying such cell lineage, which supported a novel interpretation about the common origin of mesenchymal stem cells and tissue-specific stem cells within blood vessel walls. Current research on DFAT cells indicated that this alternative source of adult multipotent cells has great potential in tissue engineering and regenerative medicine. Nature Publishing Group 2011-07 /pmc/articles/PMC3470092/ /pubmed/21789960 http://dx.doi.org/10.4248/IJOS11044 Text en Copyright © 2011 West China School of Stomatology http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Review
Shen, Jie-fei
Sugawara, Atsunori
Yamashita, Joe
Ogura, Hideo
Sato, Soh
Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues
title Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues
title_full Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues
title_fullStr Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues
title_full_unstemmed Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues
title_short Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues
title_sort dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3470092/
https://www.ncbi.nlm.nih.gov/pubmed/21789960
http://dx.doi.org/10.4248/IJOS11044
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