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Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues
When adipose-derived stem cells (ASCs) are retrieved from the stromal vascular portion of adipose tissue, a large amount of mature adipocytes are often discarded. However, by modified ceiling culture technique based on their buoyancy, mature adipocytes can be easily isolated from the adipose cell su...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3470092/ https://www.ncbi.nlm.nih.gov/pubmed/21789960 http://dx.doi.org/10.4248/IJOS11044 |
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author | Shen, Jie-fei Sugawara, Atsunori Yamashita, Joe Ogura, Hideo Sato, Soh |
author_facet | Shen, Jie-fei Sugawara, Atsunori Yamashita, Joe Ogura, Hideo Sato, Soh |
author_sort | Shen, Jie-fei |
collection | PubMed |
description | When adipose-derived stem cells (ASCs) are retrieved from the stromal vascular portion of adipose tissue, a large amount of mature adipocytes are often discarded. However, by modified ceiling culture technique based on their buoyancy, mature adipocytes can be easily isolated from the adipose cell suspension and dedifferentiated into lipid-free fibroblast-like cells, named dedifferentiated fat (DFAT) cells. DFAT cells re-establish active proliferation ability and undertake multipotent capacities. Compared with ASCs and other adult stem cells, DFAT cells showed unique advantages in their abundance, isolation and homogeneity. In this concise review, the establishment and culture methods of DFAT cells are introduced and the current profiles of their cellular nature are summarized. Under proper induction culture in vitro or environment in vivo, DFAT cells could demonstrate adipogenic, osteogenic, chondrogenic and myogenic potentials. In angiogenic conditions, DFAT cells could exhibit perivascular characteristics and elicit neovascularization. Our preliminary findings also suggested the pericyte phenotype underlying such cell lineage, which supported a novel interpretation about the common origin of mesenchymal stem cells and tissue-specific stem cells within blood vessel walls. Current research on DFAT cells indicated that this alternative source of adult multipotent cells has great potential in tissue engineering and regenerative medicine. |
format | Online Article Text |
id | pubmed-3470092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-34700922012-10-12 Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues Shen, Jie-fei Sugawara, Atsunori Yamashita, Joe Ogura, Hideo Sato, Soh Int J Oral Sci Review When adipose-derived stem cells (ASCs) are retrieved from the stromal vascular portion of adipose tissue, a large amount of mature adipocytes are often discarded. However, by modified ceiling culture technique based on their buoyancy, mature adipocytes can be easily isolated from the adipose cell suspension and dedifferentiated into lipid-free fibroblast-like cells, named dedifferentiated fat (DFAT) cells. DFAT cells re-establish active proliferation ability and undertake multipotent capacities. Compared with ASCs and other adult stem cells, DFAT cells showed unique advantages in their abundance, isolation and homogeneity. In this concise review, the establishment and culture methods of DFAT cells are introduced and the current profiles of their cellular nature are summarized. Under proper induction culture in vitro or environment in vivo, DFAT cells could demonstrate adipogenic, osteogenic, chondrogenic and myogenic potentials. In angiogenic conditions, DFAT cells could exhibit perivascular characteristics and elicit neovascularization. Our preliminary findings also suggested the pericyte phenotype underlying such cell lineage, which supported a novel interpretation about the common origin of mesenchymal stem cells and tissue-specific stem cells within blood vessel walls. Current research on DFAT cells indicated that this alternative source of adult multipotent cells has great potential in tissue engineering and regenerative medicine. Nature Publishing Group 2011-07 /pmc/articles/PMC3470092/ /pubmed/21789960 http://dx.doi.org/10.4248/IJOS11044 Text en Copyright © 2011 West China School of Stomatology http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Review Shen, Jie-fei Sugawara, Atsunori Yamashita, Joe Ogura, Hideo Sato, Soh Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues |
title | Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues |
title_full | Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues |
title_fullStr | Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues |
title_full_unstemmed | Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues |
title_short | Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues |
title_sort | dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3470092/ https://www.ncbi.nlm.nih.gov/pubmed/21789960 http://dx.doi.org/10.4248/IJOS11044 |
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