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Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues

MicroRNAs (miRNAs) have been demonstrated to play an important role in regulation of the immuno-inflammatory response; however, the function of miRNAs in periodontal inflammation has not been investigated. The objective of this study was to explore the properties of miRNAs in periodontal inflammatio...

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Autores principales: Xie, Yu-feng, Shu, Rong, Jiang, Shao-yun, Liu, Da-li, Zhang, Xiu-li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3470093/
https://www.ncbi.nlm.nih.gov/pubmed/21789961
http://dx.doi.org/10.4248/IJOS11046
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author Xie, Yu-feng
Shu, Rong
Jiang, Shao-yun
Liu, Da-li
Zhang, Xiu-li
author_facet Xie, Yu-feng
Shu, Rong
Jiang, Shao-yun
Liu, Da-li
Zhang, Xiu-li
author_sort Xie, Yu-feng
collection PubMed
description MicroRNAs (miRNAs) have been demonstrated to play an important role in regulation of the immuno-inflammatory response; however, the function of miRNAs in periodontal inflammation has not been investigated. The objective of this study was to explore the properties of miRNAs in periodontal inflammation by comparing miRNA profiles of inflamed and healthy gingival tissues. Gingival tissues were obtained from 10 periodontitis patients and 10 healthy subjects. After RNA extraction, miRNA profiles were analyzed by microarray, and expression levels of selected miRNAs were confirmed by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Analyses using two computational methods, Targetscan and MicroRNA.org, were combined to identify common targets of these miRNAs. Finally, the individual miRNA expression levels of three toll-like receptor (TLR)-related miRNAs from inflamed and healthy gingival tissues were evaluated by RT-PCR. Ninety-one miRNAs were found to be upregulated and thirty-four downregulated over two-fold in inflamed gingival tissue compared with those in healthy gingival tissue. Twelve selected inflammatory-related miRNAs, hsa-miR-126*, hsa-miR-20a, hsa-miR-142-3p, hsa-miR-19a, hsa-let-7f, hsa-miR-203, hsa-miR-17, hsa-miR-223, hsa-miR-146b, hsa-miR-146a, hsa-miR-155, and hsa-miR-205 showed comparable expression levels by microarray and real-time quantitative RT-PCR analyses. In addition, the putative inflammation targets of these miRNAs were predicted, and three that were tested (hsa-miRNA-146a, hsa-miRNA-146b, and hsa-miRNA-155), showed significant differences between inflamed and healthy gingiva. This remarkable difference in miRNA profiles between periodontal diseased and healthy gingiva implicates a probable close relationship between miRNAs and periodontal inflammation. The data also suggest that the regulation of TLRs in periodontal inflammation may involve miRNA pathways.
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spelling pubmed-34700932012-10-12 Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues Xie, Yu-feng Shu, Rong Jiang, Shao-yun Liu, Da-li Zhang, Xiu-li Int J Oral Sci Original Article MicroRNAs (miRNAs) have been demonstrated to play an important role in regulation of the immuno-inflammatory response; however, the function of miRNAs in periodontal inflammation has not been investigated. The objective of this study was to explore the properties of miRNAs in periodontal inflammation by comparing miRNA profiles of inflamed and healthy gingival tissues. Gingival tissues were obtained from 10 periodontitis patients and 10 healthy subjects. After RNA extraction, miRNA profiles were analyzed by microarray, and expression levels of selected miRNAs were confirmed by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Analyses using two computational methods, Targetscan and MicroRNA.org, were combined to identify common targets of these miRNAs. Finally, the individual miRNA expression levels of three toll-like receptor (TLR)-related miRNAs from inflamed and healthy gingival tissues were evaluated by RT-PCR. Ninety-one miRNAs were found to be upregulated and thirty-four downregulated over two-fold in inflamed gingival tissue compared with those in healthy gingival tissue. Twelve selected inflammatory-related miRNAs, hsa-miR-126*, hsa-miR-20a, hsa-miR-142-3p, hsa-miR-19a, hsa-let-7f, hsa-miR-203, hsa-miR-17, hsa-miR-223, hsa-miR-146b, hsa-miR-146a, hsa-miR-155, and hsa-miR-205 showed comparable expression levels by microarray and real-time quantitative RT-PCR analyses. In addition, the putative inflammation targets of these miRNAs were predicted, and three that were tested (hsa-miRNA-146a, hsa-miRNA-146b, and hsa-miRNA-155), showed significant differences between inflamed and healthy gingiva. This remarkable difference in miRNA profiles between periodontal diseased and healthy gingiva implicates a probable close relationship between miRNAs and periodontal inflammation. The data also suggest that the regulation of TLRs in periodontal inflammation may involve miRNA pathways. Nature Publishing Group 2011-07 /pmc/articles/PMC3470093/ /pubmed/21789961 http://dx.doi.org/10.4248/IJOS11046 Text en Copyright © 2011 West China School of Stomatology http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Original Article
Xie, Yu-feng
Shu, Rong
Jiang, Shao-yun
Liu, Da-li
Zhang, Xiu-li
Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues
title Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues
title_full Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues
title_fullStr Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues
title_full_unstemmed Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues
title_short Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues
title_sort comparison of microrna profiles of human periodontal diseased and healthy gingival tissues
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3470093/
https://www.ncbi.nlm.nih.gov/pubmed/21789961
http://dx.doi.org/10.4248/IJOS11046
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