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A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice

The maize Activator (Ac)/Dissociation (Ds) transposable element system has been used in a variety of plants for insertional mutagenesis. Ac/Ds elements can also generate genome rearrangements via alternative transposition reactions which involve the termini of closely linked transposons. Here, we in...

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Autores principales: Yu, Chuanhe, Han, Fangpu, Zhang, Jianbo, Birchler, James, Peterson, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3470690/
https://www.ncbi.nlm.nih.gov/pubmed/22798058
http://dx.doi.org/10.1007/s00122-012-1925-4
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author Yu, Chuanhe
Han, Fangpu
Zhang, Jianbo
Birchler, James
Peterson, Thomas
author_facet Yu, Chuanhe
Han, Fangpu
Zhang, Jianbo
Birchler, James
Peterson, Thomas
author_sort Yu, Chuanhe
collection PubMed
description The maize Activator (Ac)/Dissociation (Ds) transposable element system has been used in a variety of plants for insertional mutagenesis. Ac/Ds elements can also generate genome rearrangements via alternative transposition reactions which involve the termini of closely linked transposons. Here, we introduced a transgene containing reverse-oriented Ac/Ds termini together with an Ac transposase gene into rice (Oryza sativa ssp. japonica cv. Nipponbare). Among the transgenic progeny, we identified and characterized 25 independent genome rearrangements at three different chromosomal loci. The rearrangements include chromosomal deletions and inversions and one translocation. Most of the deletions occurred within the T-DNA region, but two cases showed the loss of 72 kilobase pairs (kb) and 79 kb of rice genomic DNA flanking the transgene. In addition to deletions, we obtained chromosomal inversions ranging in size from less than 10 kb (within the transgene DNA) to over 1 million base pairs (Mb). For 11 inversions, we cloned and sequenced both inversion breakpoints; in all 11 cases, the inversion junctions contained the typical 8 base pairs (bp) Ac/Ds target site duplications, confirming their origin as transposition products. Together, our results indicate that alternative Ac/Ds transposition can be an efficient tool for functional genomics and chromosomal manipulation in rice. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00122-012-1925-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-34706902012-10-18 A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice Yu, Chuanhe Han, Fangpu Zhang, Jianbo Birchler, James Peterson, Thomas Theor Appl Genet Original Paper The maize Activator (Ac)/Dissociation (Ds) transposable element system has been used in a variety of plants for insertional mutagenesis. Ac/Ds elements can also generate genome rearrangements via alternative transposition reactions which involve the termini of closely linked transposons. Here, we introduced a transgene containing reverse-oriented Ac/Ds termini together with an Ac transposase gene into rice (Oryza sativa ssp. japonica cv. Nipponbare). Among the transgenic progeny, we identified and characterized 25 independent genome rearrangements at three different chromosomal loci. The rearrangements include chromosomal deletions and inversions and one translocation. Most of the deletions occurred within the T-DNA region, but two cases showed the loss of 72 kilobase pairs (kb) and 79 kb of rice genomic DNA flanking the transgene. In addition to deletions, we obtained chromosomal inversions ranging in size from less than 10 kb (within the transgene DNA) to over 1 million base pairs (Mb). For 11 inversions, we cloned and sequenced both inversion breakpoints; in all 11 cases, the inversion junctions contained the typical 8 base pairs (bp) Ac/Ds target site duplications, confirming their origin as transposition products. Together, our results indicate that alternative Ac/Ds transposition can be an efficient tool for functional genomics and chromosomal manipulation in rice. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00122-012-1925-4) contains supplementary material, which is available to authorized users. Springer-Verlag 2012-07-14 2012 /pmc/articles/PMC3470690/ /pubmed/22798058 http://dx.doi.org/10.1007/s00122-012-1925-4 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Yu, Chuanhe
Han, Fangpu
Zhang, Jianbo
Birchler, James
Peterson, Thomas
A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice
title A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice
title_full A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice
title_fullStr A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice
title_full_unstemmed A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice
title_short A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice
title_sort transgenic system for generation of transposon ac/ds-induced chromosome rearrangements in rice
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3470690/
https://www.ncbi.nlm.nih.gov/pubmed/22798058
http://dx.doi.org/10.1007/s00122-012-1925-4
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