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Identification of a complex genetic network underlying Saccharomyces cerevisiae colony morphology

When grown on solid substrates, different microorganisms often form colonies with very specific morphologies. Whereas the pioneers of microbiology often used colony morphology to discriminate between species and strains, the phenomenon has not received much attention recently. In this study, we use...

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Autores principales: Voordeckers, Karin, De Maeyer, Dries, Zande, Elisa, Vinces, Marcelo D, Meert, Wim, Cloots, Lore, Ryan, Owen, Marchal, Kathleen, Verstrepen, Kevin J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3470922/
https://www.ncbi.nlm.nih.gov/pubmed/22882838
http://dx.doi.org/10.1111/j.1365-2958.2012.08192.x
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author Voordeckers, Karin
De Maeyer, Dries
Zande, Elisa
Vinces, Marcelo D
Meert, Wim
Cloots, Lore
Ryan, Owen
Marchal, Kathleen
Verstrepen, Kevin J
author_facet Voordeckers, Karin
De Maeyer, Dries
Zande, Elisa
Vinces, Marcelo D
Meert, Wim
Cloots, Lore
Ryan, Owen
Marchal, Kathleen
Verstrepen, Kevin J
author_sort Voordeckers, Karin
collection PubMed
description When grown on solid substrates, different microorganisms often form colonies with very specific morphologies. Whereas the pioneers of microbiology often used colony morphology to discriminate between species and strains, the phenomenon has not received much attention recently. In this study, we use a genome-wide assay in the model yeast Saccharomyces cerevisiae to identify all genes that affect colony morphology. We show that several major signalling cascades, including the MAPK, TORC, SNF1 and RIM101 pathways play a role, indicating that morphological changes are a reaction to changing environments. Other genes that affect colony morphology are involved in protein sorting and epigenetic regulation. Interestingly, the screen reveals only few genes that are likely to play a direct role in establishing colony morphology, with one notable example being FLO11, a gene encoding a cell-surface adhesin that has already been implicated in colony morphology, biofilm formation, and invasive and pseudohyphal growth. Using a series of modified promoters for fine-tuning FLO11 expression, we confirm the central role of Flo11 and show that differences in FLO11 expression result in distinct colony morphologies. Together, our results provide a first comprehensive look at the complex genetic network that underlies the diversity in the morphologies of yeast colonies.
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spelling pubmed-34709222012-10-18 Identification of a complex genetic network underlying Saccharomyces cerevisiae colony morphology Voordeckers, Karin De Maeyer, Dries Zande, Elisa Vinces, Marcelo D Meert, Wim Cloots, Lore Ryan, Owen Marchal, Kathleen Verstrepen, Kevin J Mol Microbiol Research Articles When grown on solid substrates, different microorganisms often form colonies with very specific morphologies. Whereas the pioneers of microbiology often used colony morphology to discriminate between species and strains, the phenomenon has not received much attention recently. In this study, we use a genome-wide assay in the model yeast Saccharomyces cerevisiae to identify all genes that affect colony morphology. We show that several major signalling cascades, including the MAPK, TORC, SNF1 and RIM101 pathways play a role, indicating that morphological changes are a reaction to changing environments. Other genes that affect colony morphology are involved in protein sorting and epigenetic regulation. Interestingly, the screen reveals only few genes that are likely to play a direct role in establishing colony morphology, with one notable example being FLO11, a gene encoding a cell-surface adhesin that has already been implicated in colony morphology, biofilm formation, and invasive and pseudohyphal growth. Using a series of modified promoters for fine-tuning FLO11 expression, we confirm the central role of Flo11 and show that differences in FLO11 expression result in distinct colony morphologies. Together, our results provide a first comprehensive look at the complex genetic network that underlies the diversity in the morphologies of yeast colonies. Blackwell Publishing Ltd 2012-10 2012-09-13 /pmc/articles/PMC3470922/ /pubmed/22882838 http://dx.doi.org/10.1111/j.1365-2958.2012.08192.x Text en Copyright © 2012 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Research Articles
Voordeckers, Karin
De Maeyer, Dries
Zande, Elisa
Vinces, Marcelo D
Meert, Wim
Cloots, Lore
Ryan, Owen
Marchal, Kathleen
Verstrepen, Kevin J
Identification of a complex genetic network underlying Saccharomyces cerevisiae colony morphology
title Identification of a complex genetic network underlying Saccharomyces cerevisiae colony morphology
title_full Identification of a complex genetic network underlying Saccharomyces cerevisiae colony morphology
title_fullStr Identification of a complex genetic network underlying Saccharomyces cerevisiae colony morphology
title_full_unstemmed Identification of a complex genetic network underlying Saccharomyces cerevisiae colony morphology
title_short Identification of a complex genetic network underlying Saccharomyces cerevisiae colony morphology
title_sort identification of a complex genetic network underlying saccharomyces cerevisiae colony morphology
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3470922/
https://www.ncbi.nlm.nih.gov/pubmed/22882838
http://dx.doi.org/10.1111/j.1365-2958.2012.08192.x
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