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Intrabone Transplant of Cord Blood Stem Cells Establishes a Local Engraftment Store: A Functional PET/FDG Study

Background. Despite advancements in comprehension of molecular mechanisms governing bone marrow (BM) homing of hematopoietic stem cells, cord blood transplant (CBT) suffers from a slow rate of hematopoietic recovery. Intrabone (IB) injection has been proposed as a method able to improve speed of BM...

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Autores principales: Marini, Cecilia, Podestà, Marina, Massollo, Michela, Capitanio, Selene, Fiz, Francesco, Morbelli, Silvia, Brignone, Massimo, Bacigalupo, Andrea, Piana, Michele, Frassoni, Francesco, Sambuceti, Gianmario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471032/
https://www.ncbi.nlm.nih.gov/pubmed/23093864
http://dx.doi.org/10.1155/2012/767369
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author Marini, Cecilia
Podestà, Marina
Massollo, Michela
Capitanio, Selene
Fiz, Francesco
Morbelli, Silvia
Brignone, Massimo
Bacigalupo, Andrea
Piana, Michele
Frassoni, Francesco
Sambuceti, Gianmario
author_facet Marini, Cecilia
Podestà, Marina
Massollo, Michela
Capitanio, Selene
Fiz, Francesco
Morbelli, Silvia
Brignone, Massimo
Bacigalupo, Andrea
Piana, Michele
Frassoni, Francesco
Sambuceti, Gianmario
author_sort Marini, Cecilia
collection PubMed
description Background. Despite advancements in comprehension of molecular mechanisms governing bone marrow (BM) homing of hematopoietic stem cells, cord blood transplant (CBT) suffers from a slow rate of hematopoietic recovery. Intrabone (IB) injection has been proposed as a method able to improve speed of BM engraftment with respect to conventional IV protocols. However, the mechanisms underlying this benefit are largely unknown. Aim. To verify whether IB-CBT determines a local engraftment able to predict the reconstitution of recipient hematopoiesis. Design and Methods. Twenty-one patients with hematologic malignancies received IB injection into both iliac crests of 3.2 ± 0.68 ∗ 107/kg cord blood cells. One month following IB-CBT, PET-CT imaging was performed. Maximal standardized uptake values (SUVs) were assessed in BM of both iliac crests and in all lumbar vertebrae. Results. Maximal SUV within iliac crests was higher than in lumbar vertebrae (4.1 ± 1.7 versus 3.2 ± 0.7, resp., P = 0.01). However, metabolic activity in these two different BM districts was significantly correlated (r = 0.7, P < 0.001). Moreover, FDG uptake values within the injection site closely predicted platelet recovery 100 days after IB-CBT (r = 0.72, P < 0.01). Conclusions. The metabolic activity of injected BM predicts the subsequent rate of hematopoietic recovery after IB-CBT, suggesting a pivotal role of the local engraftment in the reconstitution of recipient hematopoiesis.
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spelling pubmed-34710322012-10-23 Intrabone Transplant of Cord Blood Stem Cells Establishes a Local Engraftment Store: A Functional PET/FDG Study Marini, Cecilia Podestà, Marina Massollo, Michela Capitanio, Selene Fiz, Francesco Morbelli, Silvia Brignone, Massimo Bacigalupo, Andrea Piana, Michele Frassoni, Francesco Sambuceti, Gianmario J Biomed Biotechnol Research Article Background. Despite advancements in comprehension of molecular mechanisms governing bone marrow (BM) homing of hematopoietic stem cells, cord blood transplant (CBT) suffers from a slow rate of hematopoietic recovery. Intrabone (IB) injection has been proposed as a method able to improve speed of BM engraftment with respect to conventional IV protocols. However, the mechanisms underlying this benefit are largely unknown. Aim. To verify whether IB-CBT determines a local engraftment able to predict the reconstitution of recipient hematopoiesis. Design and Methods. Twenty-one patients with hematologic malignancies received IB injection into both iliac crests of 3.2 ± 0.68 ∗ 107/kg cord blood cells. One month following IB-CBT, PET-CT imaging was performed. Maximal standardized uptake values (SUVs) were assessed in BM of both iliac crests and in all lumbar vertebrae. Results. Maximal SUV within iliac crests was higher than in lumbar vertebrae (4.1 ± 1.7 versus 3.2 ± 0.7, resp., P = 0.01). However, metabolic activity in these two different BM districts was significantly correlated (r = 0.7, P < 0.001). Moreover, FDG uptake values within the injection site closely predicted platelet recovery 100 days after IB-CBT (r = 0.72, P < 0.01). Conclusions. The metabolic activity of injected BM predicts the subsequent rate of hematopoietic recovery after IB-CBT, suggesting a pivotal role of the local engraftment in the reconstitution of recipient hematopoiesis. Hindawi Publishing Corporation 2012 2012-10-02 /pmc/articles/PMC3471032/ /pubmed/23093864 http://dx.doi.org/10.1155/2012/767369 Text en Copyright © 2012 Cecilia Marini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Marini, Cecilia
Podestà, Marina
Massollo, Michela
Capitanio, Selene
Fiz, Francesco
Morbelli, Silvia
Brignone, Massimo
Bacigalupo, Andrea
Piana, Michele
Frassoni, Francesco
Sambuceti, Gianmario
Intrabone Transplant of Cord Blood Stem Cells Establishes a Local Engraftment Store: A Functional PET/FDG Study
title Intrabone Transplant of Cord Blood Stem Cells Establishes a Local Engraftment Store: A Functional PET/FDG Study
title_full Intrabone Transplant of Cord Blood Stem Cells Establishes a Local Engraftment Store: A Functional PET/FDG Study
title_fullStr Intrabone Transplant of Cord Blood Stem Cells Establishes a Local Engraftment Store: A Functional PET/FDG Study
title_full_unstemmed Intrabone Transplant of Cord Blood Stem Cells Establishes a Local Engraftment Store: A Functional PET/FDG Study
title_short Intrabone Transplant of Cord Blood Stem Cells Establishes a Local Engraftment Store: A Functional PET/FDG Study
title_sort intrabone transplant of cord blood stem cells establishes a local engraftment store: a functional pet/fdg study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471032/
https://www.ncbi.nlm.nih.gov/pubmed/23093864
http://dx.doi.org/10.1155/2012/767369
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