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Protein P7 of the Cystovirus φ6 Is Located at the Three-Fold Axis of the Unexpanded Procapsid

The objective of this study was to determine the location of protein P7, the RNA packaging factor, in the procapsid of the φ6 cystovirus. A comparison of cryo-electron microscopy high-resolution single particle reconstructions of the φ6 complete unexpanded procapsid, the protein P2-minus procapsid (...

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Autores principales: Katz, Garrett, Wei, Hui, Alimova, Alexandra, Katz, Al, Morgan, David Gene, Gottlieb, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471842/
https://www.ncbi.nlm.nih.gov/pubmed/23077625
http://dx.doi.org/10.1371/journal.pone.0047489
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author Katz, Garrett
Wei, Hui
Alimova, Alexandra
Katz, Al
Morgan, David Gene
Gottlieb, Paul
author_facet Katz, Garrett
Wei, Hui
Alimova, Alexandra
Katz, Al
Morgan, David Gene
Gottlieb, Paul
author_sort Katz, Garrett
collection PubMed
description The objective of this study was to determine the location of protein P7, the RNA packaging factor, in the procapsid of the φ6 cystovirus. A comparison of cryo-electron microscopy high-resolution single particle reconstructions of the φ6 complete unexpanded procapsid, the protein P2-minus procapsid (P2 is the RNA directed RNA-polymerase), and the P7-minus procapsid, show that prior to RNA packaging the P7 protein is located near the three-fold axis of symmetry. Difference maps highlight the precise position of P7 and demonstrate that in P7-minus particles the P2 proteins are less localized with reduced densities at the three-fold axes. We propose that P7 performs the mechanical function of stabilizing P2 on the inner protein P1 shell which ensures that entering viral single-stranded RNA is replicated.
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spelling pubmed-34718422012-10-17 Protein P7 of the Cystovirus φ6 Is Located at the Three-Fold Axis of the Unexpanded Procapsid Katz, Garrett Wei, Hui Alimova, Alexandra Katz, Al Morgan, David Gene Gottlieb, Paul PLoS One Research Article The objective of this study was to determine the location of protein P7, the RNA packaging factor, in the procapsid of the φ6 cystovirus. A comparison of cryo-electron microscopy high-resolution single particle reconstructions of the φ6 complete unexpanded procapsid, the protein P2-minus procapsid (P2 is the RNA directed RNA-polymerase), and the P7-minus procapsid, show that prior to RNA packaging the P7 protein is located near the three-fold axis of symmetry. Difference maps highlight the precise position of P7 and demonstrate that in P7-minus particles the P2 proteins are less localized with reduced densities at the three-fold axes. We propose that P7 performs the mechanical function of stabilizing P2 on the inner protein P1 shell which ensures that entering viral single-stranded RNA is replicated. Public Library of Science 2012-10-15 /pmc/articles/PMC3471842/ /pubmed/23077625 http://dx.doi.org/10.1371/journal.pone.0047489 Text en © 2012 Katz et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Katz, Garrett
Wei, Hui
Alimova, Alexandra
Katz, Al
Morgan, David Gene
Gottlieb, Paul
Protein P7 of the Cystovirus φ6 Is Located at the Three-Fold Axis of the Unexpanded Procapsid
title Protein P7 of the Cystovirus φ6 Is Located at the Three-Fold Axis of the Unexpanded Procapsid
title_full Protein P7 of the Cystovirus φ6 Is Located at the Three-Fold Axis of the Unexpanded Procapsid
title_fullStr Protein P7 of the Cystovirus φ6 Is Located at the Three-Fold Axis of the Unexpanded Procapsid
title_full_unstemmed Protein P7 of the Cystovirus φ6 Is Located at the Three-Fold Axis of the Unexpanded Procapsid
title_short Protein P7 of the Cystovirus φ6 Is Located at the Three-Fold Axis of the Unexpanded Procapsid
title_sort protein p7 of the cystovirus φ6 is located at the three-fold axis of the unexpanded procapsid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471842/
https://www.ncbi.nlm.nih.gov/pubmed/23077625
http://dx.doi.org/10.1371/journal.pone.0047489
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