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Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma

We report a proteomic analysis of microdissected material from formalin-fixed and paraffin-embedded colorectal cancer, quantifying >7500 proteins between patient matched normal mucosa, primary carcinoma, and nodal metastases. Expression levels of 1808 proteins changed significantly between normal...

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Autores principales: Wiśniewski, Jacek R, Ostasiewicz, Paweł, Duś, Kamila, Zielińska, Dorota F, Gnad, Florian, Mann, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Molecular Biology Organization 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472694/
https://www.ncbi.nlm.nih.gov/pubmed/22968445
http://dx.doi.org/10.1038/msb.2012.44
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author Wiśniewski, Jacek R
Ostasiewicz, Paweł
Duś, Kamila
Zielińska, Dorota F
Gnad, Florian
Mann, Matthias
author_facet Wiśniewski, Jacek R
Ostasiewicz, Paweł
Duś, Kamila
Zielińska, Dorota F
Gnad, Florian
Mann, Matthias
author_sort Wiśniewski, Jacek R
collection PubMed
description We report a proteomic analysis of microdissected material from formalin-fixed and paraffin-embedded colorectal cancer, quantifying >7500 proteins between patient matched normal mucosa, primary carcinoma, and nodal metastases. Expression levels of 1808 proteins changed significantly between normal and cancer tissues, a much larger fraction than that reported in transcript-based studies. Tumor cells exhibit extensive alterations in the cell-surface and nuclear proteomes. Functionally similar changes in the proteome were observed comparing rapidly growing and differentiated CaCo-2 cells. In contrast, there was minimal proteomic remodeling between primary cancer and metastases, suggesting that no drastic proteome changes are necessary for the tumor to propagate in a different tissue context. Additionally, we introduce a new way to determine protein copy numbers per cell without protein standards. Copy numbers estimated in enterocytes and cancer cells are in good agreement with CaCo-2 and HeLa cells and with the literature data. Our proteomic data set furthermore allows mapping quantitative changes of functional protein classes, enabling novel insights into the biology of colon cancer.
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spelling pubmed-34726942012-10-16 Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma Wiśniewski, Jacek R Ostasiewicz, Paweł Duś, Kamila Zielińska, Dorota F Gnad, Florian Mann, Matthias Mol Syst Biol Article We report a proteomic analysis of microdissected material from formalin-fixed and paraffin-embedded colorectal cancer, quantifying >7500 proteins between patient matched normal mucosa, primary carcinoma, and nodal metastases. Expression levels of 1808 proteins changed significantly between normal and cancer tissues, a much larger fraction than that reported in transcript-based studies. Tumor cells exhibit extensive alterations in the cell-surface and nuclear proteomes. Functionally similar changes in the proteome were observed comparing rapidly growing and differentiated CaCo-2 cells. In contrast, there was minimal proteomic remodeling between primary cancer and metastases, suggesting that no drastic proteome changes are necessary for the tumor to propagate in a different tissue context. Additionally, we introduce a new way to determine protein copy numbers per cell without protein standards. Copy numbers estimated in enterocytes and cancer cells are in good agreement with CaCo-2 and HeLa cells and with the literature data. Our proteomic data set furthermore allows mapping quantitative changes of functional protein classes, enabling novel insights into the biology of colon cancer. European Molecular Biology Organization 2012-09-11 /pmc/articles/PMC3472694/ /pubmed/22968445 http://dx.doi.org/10.1038/msb.2012.44 Text en Copyright © 2012, EMBO and Macmillan Publishers Limited https://creativecommons.org/licenses/by-nc-sa/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.
spellingShingle Article
Wiśniewski, Jacek R
Ostasiewicz, Paweł
Duś, Kamila
Zielińska, Dorota F
Gnad, Florian
Mann, Matthias
Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma
title Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma
title_full Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma
title_fullStr Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma
title_full_unstemmed Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma
title_short Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma
title_sort extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472694/
https://www.ncbi.nlm.nih.gov/pubmed/22968445
http://dx.doi.org/10.1038/msb.2012.44
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