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Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma
We report a proteomic analysis of microdissected material from formalin-fixed and paraffin-embedded colorectal cancer, quantifying >7500 proteins between patient matched normal mucosa, primary carcinoma, and nodal metastases. Expression levels of 1808 proteins changed significantly between normal...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Molecular Biology Organization
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472694/ https://www.ncbi.nlm.nih.gov/pubmed/22968445 http://dx.doi.org/10.1038/msb.2012.44 |
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author | Wiśniewski, Jacek R Ostasiewicz, Paweł Duś, Kamila Zielińska, Dorota F Gnad, Florian Mann, Matthias |
author_facet | Wiśniewski, Jacek R Ostasiewicz, Paweł Duś, Kamila Zielińska, Dorota F Gnad, Florian Mann, Matthias |
author_sort | Wiśniewski, Jacek R |
collection | PubMed |
description | We report a proteomic analysis of microdissected material from formalin-fixed and paraffin-embedded colorectal cancer, quantifying >7500 proteins between patient matched normal mucosa, primary carcinoma, and nodal metastases. Expression levels of 1808 proteins changed significantly between normal and cancer tissues, a much larger fraction than that reported in transcript-based studies. Tumor cells exhibit extensive alterations in the cell-surface and nuclear proteomes. Functionally similar changes in the proteome were observed comparing rapidly growing and differentiated CaCo-2 cells. In contrast, there was minimal proteomic remodeling between primary cancer and metastases, suggesting that no drastic proteome changes are necessary for the tumor to propagate in a different tissue context. Additionally, we introduce a new way to determine protein copy numbers per cell without protein standards. Copy numbers estimated in enterocytes and cancer cells are in good agreement with CaCo-2 and HeLa cells and with the literature data. Our proteomic data set furthermore allows mapping quantitative changes of functional protein classes, enabling novel insights into the biology of colon cancer. |
format | Online Article Text |
id | pubmed-3472694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | European Molecular Biology Organization |
record_format | MEDLINE/PubMed |
spelling | pubmed-34726942012-10-16 Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma Wiśniewski, Jacek R Ostasiewicz, Paweł Duś, Kamila Zielińska, Dorota F Gnad, Florian Mann, Matthias Mol Syst Biol Article We report a proteomic analysis of microdissected material from formalin-fixed and paraffin-embedded colorectal cancer, quantifying >7500 proteins between patient matched normal mucosa, primary carcinoma, and nodal metastases. Expression levels of 1808 proteins changed significantly between normal and cancer tissues, a much larger fraction than that reported in transcript-based studies. Tumor cells exhibit extensive alterations in the cell-surface and nuclear proteomes. Functionally similar changes in the proteome were observed comparing rapidly growing and differentiated CaCo-2 cells. In contrast, there was minimal proteomic remodeling between primary cancer and metastases, suggesting that no drastic proteome changes are necessary for the tumor to propagate in a different tissue context. Additionally, we introduce a new way to determine protein copy numbers per cell without protein standards. Copy numbers estimated in enterocytes and cancer cells are in good agreement with CaCo-2 and HeLa cells and with the literature data. Our proteomic data set furthermore allows mapping quantitative changes of functional protein classes, enabling novel insights into the biology of colon cancer. European Molecular Biology Organization 2012-09-11 /pmc/articles/PMC3472694/ /pubmed/22968445 http://dx.doi.org/10.1038/msb.2012.44 Text en Copyright © 2012, EMBO and Macmillan Publishers Limited https://creativecommons.org/licenses/by-nc-sa/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission. |
spellingShingle | Article Wiśniewski, Jacek R Ostasiewicz, Paweł Duś, Kamila Zielińska, Dorota F Gnad, Florian Mann, Matthias Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma |
title | Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma |
title_full | Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma |
title_fullStr | Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma |
title_full_unstemmed | Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma |
title_short | Extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma |
title_sort | extensive quantitative remodeling of the proteome between normal colon tissue and adenocarcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472694/ https://www.ncbi.nlm.nih.gov/pubmed/22968445 http://dx.doi.org/10.1038/msb.2012.44 |
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