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Characterization of 15 Polymorphic Microsatellite Loci for Cephalotaxus oliveri (Cephalotaxaceae), a Conifer of Medicinal Importance

Cephalotaxus oliveri is a scarce medicinal conifer endemic to the south central region of China and Vietnam. A small fragmented population presently exists due to anthropogenic disturbance. C. oliveri has been used for its alkaloids harringtonine and homoharringtonine, which are effective against le...

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Autores principales: Miao, Yingchun, Lang, Xuedong, Li, Shuaifeng, Su, Jianrong, Wang, Yuehua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472736/
https://www.ncbi.nlm.nih.gov/pubmed/23109844
http://dx.doi.org/10.3390/ijms130911165
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author Miao, Yingchun
Lang, Xuedong
Li, Shuaifeng
Su, Jianrong
Wang, Yuehua
author_facet Miao, Yingchun
Lang, Xuedong
Li, Shuaifeng
Su, Jianrong
Wang, Yuehua
author_sort Miao, Yingchun
collection PubMed
description Cephalotaxus oliveri is a scarce medicinal conifer endemic to the south central region of China and Vietnam. A small fragmented population presently exists due to anthropogenic disturbance. C. oliveri has been used for its alkaloids harringtonine and homoharringtonine, which are effective against leucocythemia and lymphadenosarcoma. Monoecious plants have been detected in nature, although they were understood to be dioecious. In order to study the mating system, population genetics and the genetic effects of habitat fragmentation on C. oliveri, 15 polymorphic and 12 monomorphic microsatellite loci were developed for C. oliveri by using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol. The polymorphisms were assessed in 96 individuals from three natural populations (32 individuals per population). The number of alleles per locus ranged from two to 33, the observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.923, respectively. These loci would facilitate a comprehensive understanding of the genetic dynamics on C. oliveri, which will be useful for establishing effective conservation strategies for this species.
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spelling pubmed-34727362012-10-29 Characterization of 15 Polymorphic Microsatellite Loci for Cephalotaxus oliveri (Cephalotaxaceae), a Conifer of Medicinal Importance Miao, Yingchun Lang, Xuedong Li, Shuaifeng Su, Jianrong Wang, Yuehua Int J Mol Sci Short Note Cephalotaxus oliveri is a scarce medicinal conifer endemic to the south central region of China and Vietnam. A small fragmented population presently exists due to anthropogenic disturbance. C. oliveri has been used for its alkaloids harringtonine and homoharringtonine, which are effective against leucocythemia and lymphadenosarcoma. Monoecious plants have been detected in nature, although they were understood to be dioecious. In order to study the mating system, population genetics and the genetic effects of habitat fragmentation on C. oliveri, 15 polymorphic and 12 monomorphic microsatellite loci were developed for C. oliveri by using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol. The polymorphisms were assessed in 96 individuals from three natural populations (32 individuals per population). The number of alleles per locus ranged from two to 33, the observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.923, respectively. These loci would facilitate a comprehensive understanding of the genetic dynamics on C. oliveri, which will be useful for establishing effective conservation strategies for this species. Molecular Diversity Preservation International (MDPI) 2012-09-07 /pmc/articles/PMC3472736/ /pubmed/23109844 http://dx.doi.org/10.3390/ijms130911165 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Short Note
Miao, Yingchun
Lang, Xuedong
Li, Shuaifeng
Su, Jianrong
Wang, Yuehua
Characterization of 15 Polymorphic Microsatellite Loci for Cephalotaxus oliveri (Cephalotaxaceae), a Conifer of Medicinal Importance
title Characterization of 15 Polymorphic Microsatellite Loci for Cephalotaxus oliveri (Cephalotaxaceae), a Conifer of Medicinal Importance
title_full Characterization of 15 Polymorphic Microsatellite Loci for Cephalotaxus oliveri (Cephalotaxaceae), a Conifer of Medicinal Importance
title_fullStr Characterization of 15 Polymorphic Microsatellite Loci for Cephalotaxus oliveri (Cephalotaxaceae), a Conifer of Medicinal Importance
title_full_unstemmed Characterization of 15 Polymorphic Microsatellite Loci for Cephalotaxus oliveri (Cephalotaxaceae), a Conifer of Medicinal Importance
title_short Characterization of 15 Polymorphic Microsatellite Loci for Cephalotaxus oliveri (Cephalotaxaceae), a Conifer of Medicinal Importance
title_sort characterization of 15 polymorphic microsatellite loci for cephalotaxus oliveri (cephalotaxaceae), a conifer of medicinal importance
topic Short Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472736/
https://www.ncbi.nlm.nih.gov/pubmed/23109844
http://dx.doi.org/10.3390/ijms130911165
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