Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome

In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean) by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphi...

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Autores principales: Wang, Aihua, Ding, Yi, Hu, Zhenhua, Lin, Chufa, Wang, Shuzhen, Wang, Bingcai, Zhang, Hongyuan, Zhou, Guolin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Short Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472738/
https://www.ncbi.nlm.nih.gov/pubmed/23109846
http://dx.doi.org/10.3390/ijms130911188
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author Wang, Aihua
Ding, Yi
Hu, Zhenhua
Lin, Chufa
Wang, Shuzhen
Wang, Bingcai
Zhang, Hongyuan
Zhou, Guolin
author_facet Wang, Aihua
Ding, Yi
Hu, Zhenhua
Lin, Chufa
Wang, Shuzhen
Wang, Bingcai
Zhang, Hongyuan
Zhou, Guolin
author_sort Wang, Aihua
collection PubMed
description In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean) by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphic information content (PIC) values ranged from 0.055 to 0.721 over 13 loci, with a mean value of 0.492, and 7 loci having PIC greater than 0.5. The expected heterozygosity (H(E)) and observed heterozygosity (H(O)) levels ranged from 0.057 to 0.814 and from 0.026 to 0.531, respectively. Cross-species amplification of the 13 prime pairs was performed in its related specie of Vigna unguiculata L. Seven out of all these markers showed cross-species transferability. These markers will be useful for future genetic diversity and population genetics studies for this agricultural specie and its related species.
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spelling pubmed-34727382012-10-29 Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome Wang, Aihua Ding, Yi Hu, Zhenhua Lin, Chufa Wang, Shuzhen Wang, Bingcai Zhang, Hongyuan Zhou, Guolin Int J Mol Sci Short Note In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean) by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphic information content (PIC) values ranged from 0.055 to 0.721 over 13 loci, with a mean value of 0.492, and 7 loci having PIC greater than 0.5. The expected heterozygosity (H(E)) and observed heterozygosity (H(O)) levels ranged from 0.057 to 0.814 and from 0.026 to 0.531, respectively. Cross-species amplification of the 13 prime pairs was performed in its related specie of Vigna unguiculata L. Seven out of all these markers showed cross-species transferability. These markers will be useful for future genetic diversity and population genetics studies for this agricultural specie and its related species. Molecular Diversity Preservation International (MDPI) 2012-09-07 /pmc/articles/PMC3472738/ /pubmed/23109846 http://dx.doi.org/10.3390/ijms130911188 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Short Note
Wang, Aihua
Ding, Yi
Hu, Zhenhua
Lin, Chufa
Wang, Shuzhen
Wang, Bingcai
Zhang, Hongyuan
Zhou, Guolin
Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome
title Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome
title_full Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome
title_fullStr Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome
title_full_unstemmed Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome
title_short Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome
title_sort isolation and characterization of 13 new polymorphic microsatellite markers in the phaseolus vulgaris l. (common bean) genome
topic Short Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472738/
https://www.ncbi.nlm.nih.gov/pubmed/23109846
http://dx.doi.org/10.3390/ijms130911188
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