Combination of Oxyanion Gln114 Mutation and Medium Engineering to Influence the Enantioselectivity of Thermophilic Lipase from Geobacillus zalihae

The substitution of the oxyanion Q114 with Met and Leu was carried out to investigate the role of Q114 in imparting enantioselectivity on T1 lipase. The mutation improved enantioselectivity in Q114M over the wild-type, while enantioselectivity in Q114L was reduced. The enantioselectivity of the ther...

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Detalles Bibliográficos
Autores principales: Wahab, Roswanira Abdul, Basri, Mahiran, Rahman, Mohd Basyaruddin Abdul, Rahman, Raja Noor Zaliha Raja Abdul, Salleh, Abu Bakar, Leow, Thean Chor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472768/
https://www.ncbi.nlm.nih.gov/pubmed/23109876
http://dx.doi.org/10.3390/ijms130911666

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472768/
https://www.ncbi.nlm.nih.gov/pubmed/23109876
http://dx.doi.org/10.3390/ijms130911666

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