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Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move

It has been a long-standing question how DNA damage repair proceeds in a nuclear environment where DNA is packaged into chromatin. Several decades of analysis combining in vitro and in vivo studies in various model organisms ranging from yeast to human have markedly increased our understanding of th...

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Detalles Bibliográficos
Autores principales: Adam, Salomé, Polo, Sophie E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472782/
https://www.ncbi.nlm.nih.gov/pubmed/23109890
http://dx.doi.org/10.3390/ijms130911895
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author Adam, Salomé
Polo, Sophie E.
author_facet Adam, Salomé
Polo, Sophie E.
author_sort Adam, Salomé
collection PubMed
description It has been a long-standing question how DNA damage repair proceeds in a nuclear environment where DNA is packaged into chromatin. Several decades of analysis combining in vitro and in vivo studies in various model organisms ranging from yeast to human have markedly increased our understanding of the mechanisms underlying chromatin disorganization upon damage detection and re-assembly after repair. Here, we review the methods that have been developed over the years to delineate chromatin alterations in response to DNA damage by focusing on the well-characterized Nucleotide Excision Repair (NER) pathway. We also highlight how these methods have provided key mechanistic insight into histone dynamics coupled to repair in mammals, raising new issues about the maintenance of chromatin integrity. In particular, we discuss how NER factors and central players in chromatin dynamics such as histone modifiers, nucleosome remodeling factors, and histone chaperones function to mobilize histones during repair.
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spelling pubmed-34727822012-10-29 Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move Adam, Salomé Polo, Sophie E. Int J Mol Sci Review It has been a long-standing question how DNA damage repair proceeds in a nuclear environment where DNA is packaged into chromatin. Several decades of analysis combining in vitro and in vivo studies in various model organisms ranging from yeast to human have markedly increased our understanding of the mechanisms underlying chromatin disorganization upon damage detection and re-assembly after repair. Here, we review the methods that have been developed over the years to delineate chromatin alterations in response to DNA damage by focusing on the well-characterized Nucleotide Excision Repair (NER) pathway. We also highlight how these methods have provided key mechanistic insight into histone dynamics coupled to repair in mammals, raising new issues about the maintenance of chromatin integrity. In particular, we discuss how NER factors and central players in chromatin dynamics such as histone modifiers, nucleosome remodeling factors, and histone chaperones function to mobilize histones during repair. Molecular Diversity Preservation International (MDPI) 2012-09-19 /pmc/articles/PMC3472782/ /pubmed/23109890 http://dx.doi.org/10.3390/ijms130911895 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Adam, Salomé
Polo, Sophie E.
Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move
title Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move
title_full Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move
title_fullStr Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move
title_full_unstemmed Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move
title_short Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move
title_sort chromatin dynamics during nucleotide excision repair: histones on the move
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472782/
https://www.ncbi.nlm.nih.gov/pubmed/23109890
http://dx.doi.org/10.3390/ijms130911895
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