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Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move
It has been a long-standing question how DNA damage repair proceeds in a nuclear environment where DNA is packaged into chromatin. Several decades of analysis combining in vitro and in vivo studies in various model organisms ranging from yeast to human have markedly increased our understanding of th...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472782/ https://www.ncbi.nlm.nih.gov/pubmed/23109890 http://dx.doi.org/10.3390/ijms130911895 |
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author | Adam, Salomé Polo, Sophie E. |
author_facet | Adam, Salomé Polo, Sophie E. |
author_sort | Adam, Salomé |
collection | PubMed |
description | It has been a long-standing question how DNA damage repair proceeds in a nuclear environment where DNA is packaged into chromatin. Several decades of analysis combining in vitro and in vivo studies in various model organisms ranging from yeast to human have markedly increased our understanding of the mechanisms underlying chromatin disorganization upon damage detection and re-assembly after repair. Here, we review the methods that have been developed over the years to delineate chromatin alterations in response to DNA damage by focusing on the well-characterized Nucleotide Excision Repair (NER) pathway. We also highlight how these methods have provided key mechanistic insight into histone dynamics coupled to repair in mammals, raising new issues about the maintenance of chromatin integrity. In particular, we discuss how NER factors and central players in chromatin dynamics such as histone modifiers, nucleosome remodeling factors, and histone chaperones function to mobilize histones during repair. |
format | Online Article Text |
id | pubmed-3472782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-34727822012-10-29 Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move Adam, Salomé Polo, Sophie E. Int J Mol Sci Review It has been a long-standing question how DNA damage repair proceeds in a nuclear environment where DNA is packaged into chromatin. Several decades of analysis combining in vitro and in vivo studies in various model organisms ranging from yeast to human have markedly increased our understanding of the mechanisms underlying chromatin disorganization upon damage detection and re-assembly after repair. Here, we review the methods that have been developed over the years to delineate chromatin alterations in response to DNA damage by focusing on the well-characterized Nucleotide Excision Repair (NER) pathway. We also highlight how these methods have provided key mechanistic insight into histone dynamics coupled to repair in mammals, raising new issues about the maintenance of chromatin integrity. In particular, we discuss how NER factors and central players in chromatin dynamics such as histone modifiers, nucleosome remodeling factors, and histone chaperones function to mobilize histones during repair. Molecular Diversity Preservation International (MDPI) 2012-09-19 /pmc/articles/PMC3472782/ /pubmed/23109890 http://dx.doi.org/10.3390/ijms130911895 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Review Adam, Salomé Polo, Sophie E. Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move |
title | Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move |
title_full | Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move |
title_fullStr | Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move |
title_full_unstemmed | Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move |
title_short | Chromatin Dynamics during Nucleotide Excision Repair: Histones on the Move |
title_sort | chromatin dynamics during nucleotide excision repair: histones on the move |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472782/ https://www.ncbi.nlm.nih.gov/pubmed/23109890 http://dx.doi.org/10.3390/ijms130911895 |
work_keys_str_mv | AT adamsalome chromatindynamicsduringnucleotideexcisionrepairhistonesonthemove AT polosophiee chromatindynamicsduringnucleotideexcisionrepairhistonesonthemove |