Comparing Proteomics and RISC Immunoprecipitations to Identify Targets of Epstein-Barr Viral miRNAs

Epstein-Barr virus is a gamma-herpes virus that is causally associated with several lymphomas and carcinomas. This virus encodes at least 25 pre-miRNAs, which are expressed in infected cells to yield more than 50 detected mature miRNAs. miRNAs are small, non-coding RNAs that inhibit gene expression...

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Autores principales: Kuzembayeva, Malika, Chiu, Ya-Fang, Sugden, Bill
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472983/
https://www.ncbi.nlm.nih.gov/pubmed/23091622
http://dx.doi.org/10.1371/journal.pone.0047409
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author Kuzembayeva, Malika
Chiu, Ya-Fang
Sugden, Bill
author_facet Kuzembayeva, Malika
Chiu, Ya-Fang
Sugden, Bill
author_sort Kuzembayeva, Malika
collection PubMed
description Epstein-Barr virus is a gamma-herpes virus that is causally associated with several lymphomas and carcinomas. This virus encodes at least 25 pre-miRNAs, which are expressed in infected cells to yield more than 50 detected mature miRNAs. miRNAs are small, non-coding RNAs that inhibit gene expression by promoting the inhibition of translation or of degradation of mRNAs. Currently, the function of these viral miRNAs and the contribution they provide to EBV’s life-cycle remain largely unknown, due to difficulties in identifying cellular and viral genes regulated by these miRNAs. We have compared and contrasted two methods to identify targets of viral miRNAs in order to identify the advantages and limitations of each method to aid in uncovering the functions of EBV’s miRNAs.
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spelling pubmed-34729832012-10-22 Comparing Proteomics and RISC Immunoprecipitations to Identify Targets of Epstein-Barr Viral miRNAs Kuzembayeva, Malika Chiu, Ya-Fang Sugden, Bill PLoS One Research Article Epstein-Barr virus is a gamma-herpes virus that is causally associated with several lymphomas and carcinomas. This virus encodes at least 25 pre-miRNAs, which are expressed in infected cells to yield more than 50 detected mature miRNAs. miRNAs are small, non-coding RNAs that inhibit gene expression by promoting the inhibition of translation or of degradation of mRNAs. Currently, the function of these viral miRNAs and the contribution they provide to EBV’s life-cycle remain largely unknown, due to difficulties in identifying cellular and viral genes regulated by these miRNAs. We have compared and contrasted two methods to identify targets of viral miRNAs in order to identify the advantages and limitations of each method to aid in uncovering the functions of EBV’s miRNAs. Public Library of Science 2012-10-16 /pmc/articles/PMC3472983/ /pubmed/23091622 http://dx.doi.org/10.1371/journal.pone.0047409 Text en © 2012 Kuzembayeva et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kuzembayeva, Malika
Chiu, Ya-Fang
Sugden, Bill
Comparing Proteomics and RISC Immunoprecipitations to Identify Targets of Epstein-Barr Viral miRNAs
title Comparing Proteomics and RISC Immunoprecipitations to Identify Targets of Epstein-Barr Viral miRNAs
title_full Comparing Proteomics and RISC Immunoprecipitations to Identify Targets of Epstein-Barr Viral miRNAs
title_fullStr Comparing Proteomics and RISC Immunoprecipitations to Identify Targets of Epstein-Barr Viral miRNAs
title_full_unstemmed Comparing Proteomics and RISC Immunoprecipitations to Identify Targets of Epstein-Barr Viral miRNAs
title_short Comparing Proteomics and RISC Immunoprecipitations to Identify Targets of Epstein-Barr Viral miRNAs
title_sort comparing proteomics and risc immunoprecipitations to identify targets of epstein-barr viral mirnas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472983/
https://www.ncbi.nlm.nih.gov/pubmed/23091622
http://dx.doi.org/10.1371/journal.pone.0047409
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