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Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis

Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell....

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Detalles Bibliográficos
Autores principales: Chen, Zhong-Hua, Eisenach, Cornelia, Xu, Xin-Qin, Hills, Adrian, Blatt, Michael R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475070/
https://www.ncbi.nlm.nih.gov/pubmed/22559714
http://dx.doi.org/10.1186/1746-4811-8-15
Descripción
Sumario:Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K(+) and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.