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Antinociceptive and Anti-Inflammatory Activities of Crude Methanolic Extract of Red Alga Bryothamnion triquetrum

The marine environment is an extraordinary reservoir of bioactive natural products, many of which exhibit chemical and structural features not found in terrestrial natural products. In this regard, the aim of this study was to investigate the possible antinociceptive and anti-inflammatory activities...

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Autores principales: Cavalcante-Silva, Luiz Henrique Agra, da Matta, Carolina Barbosa Brito, de Araújo, Morgana Vital, Barbosa-Filho, José Maria, de Lira, Daysianne Pereira, de Oliveira Santos, Bárbara Viviana, de Miranda, George Emmanuel C., Alexandre-Moreira, Magna Suzana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475267/
https://www.ncbi.nlm.nih.gov/pubmed/23118715
http://dx.doi.org/10.3390/md10091977
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author Cavalcante-Silva, Luiz Henrique Agra
da Matta, Carolina Barbosa Brito
de Araújo, Morgana Vital
Barbosa-Filho, José Maria
de Lira, Daysianne Pereira
de Oliveira Santos, Bárbara Viviana
de Miranda, George Emmanuel C.
Alexandre-Moreira, Magna Suzana
author_facet Cavalcante-Silva, Luiz Henrique Agra
da Matta, Carolina Barbosa Brito
de Araújo, Morgana Vital
Barbosa-Filho, José Maria
de Lira, Daysianne Pereira
de Oliveira Santos, Bárbara Viviana
de Miranda, George Emmanuel C.
Alexandre-Moreira, Magna Suzana
author_sort Cavalcante-Silva, Luiz Henrique Agra
collection PubMed
description The marine environment is an extraordinary reservoir of bioactive natural products, many of which exhibit chemical and structural features not found in terrestrial natural products. In this regard, the aim of this study was to investigate the possible antinociceptive and anti-inflammatory activities of a crude methanolic extract of the red alga Bryothamnion triquetrum (BT-MeOH) in murine models. Groups of Swiss mice of both sexes (25–30 g) were used throughout the experiments. The potential antinociceptive of BT-MeOH was evaluated by means of the following tests: acetic acid-induced writhing, hot-plate test and glutamate- and formalin-induced nociception. The anti-inflammatory activity of BT-MeOH was investigated using the zymosan A-induced peritonitis test. The tests were conducted using 100 mg/kg (p.o.) BT-MeOH, 33.3 mg/kg (p.o.) dipyrone, 35.7 mg/kg (p.o.) indomethacin and 5.7 mg/kg (s.c.) morphine. The extract and all standard drugs were administered 40 min before the nociceptive/inflammatory stimulus. In the acetic acid-induced writhing test, BT-MeOH and dipyrone inhibited the nociceptive response by 55.9% (22.2 ± 2.0 writhings; p < 0.01) and 80.9% (9.6 ± 2.1 writhings; p < 0.01). In the hot-plate test, BT-MeOH did not increase the latency time of the animals in the time evaluated. In addition, BT-MeOH inhibited glutamate-induced nociception by 50.1%. While BT-MeOH did not inhibit the neurogenic phase in formalin-induced nociception, the inflammatory phase was inhibited by 53.1% (66.8 ± 14.2 s; p < 0.01). Indomethacin inhibited the inflammatory phase by 60.2% (56.8 ± 8.7 s; p < 0.01). In the zymosan-induced peritonitis test, BT-MeOH inhibited 55.6% (6.6 ± 0.2 × 10(6) leukocytes/mL; p < 0.01) of leukocyte migration, while indomethacin inhibited 78.1% (3.2 ± 0.1 × 10(6) leukocytes/mL; p < 0.01). Based on the results obtained in this study, we conclude that BT-MeOH has peripheral antinociceptive and anti-inflammatory activities. However, more studies need to be conducted to confirm these properties.
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spelling pubmed-34752672012-11-01 Antinociceptive and Anti-Inflammatory Activities of Crude Methanolic Extract of Red Alga Bryothamnion triquetrum Cavalcante-Silva, Luiz Henrique Agra da Matta, Carolina Barbosa Brito de Araújo, Morgana Vital Barbosa-Filho, José Maria de Lira, Daysianne Pereira de Oliveira Santos, Bárbara Viviana de Miranda, George Emmanuel C. Alexandre-Moreira, Magna Suzana Mar Drugs Article The marine environment is an extraordinary reservoir of bioactive natural products, many of which exhibit chemical and structural features not found in terrestrial natural products. In this regard, the aim of this study was to investigate the possible antinociceptive and anti-inflammatory activities of a crude methanolic extract of the red alga Bryothamnion triquetrum (BT-MeOH) in murine models. Groups of Swiss mice of both sexes (25–30 g) were used throughout the experiments. The potential antinociceptive of BT-MeOH was evaluated by means of the following tests: acetic acid-induced writhing, hot-plate test and glutamate- and formalin-induced nociception. The anti-inflammatory activity of BT-MeOH was investigated using the zymosan A-induced peritonitis test. The tests were conducted using 100 mg/kg (p.o.) BT-MeOH, 33.3 mg/kg (p.o.) dipyrone, 35.7 mg/kg (p.o.) indomethacin and 5.7 mg/kg (s.c.) morphine. The extract and all standard drugs were administered 40 min before the nociceptive/inflammatory stimulus. In the acetic acid-induced writhing test, BT-MeOH and dipyrone inhibited the nociceptive response by 55.9% (22.2 ± 2.0 writhings; p < 0.01) and 80.9% (9.6 ± 2.1 writhings; p < 0.01). In the hot-plate test, BT-MeOH did not increase the latency time of the animals in the time evaluated. In addition, BT-MeOH inhibited glutamate-induced nociception by 50.1%. While BT-MeOH did not inhibit the neurogenic phase in formalin-induced nociception, the inflammatory phase was inhibited by 53.1% (66.8 ± 14.2 s; p < 0.01). Indomethacin inhibited the inflammatory phase by 60.2% (56.8 ± 8.7 s; p < 0.01). In the zymosan-induced peritonitis test, BT-MeOH inhibited 55.6% (6.6 ± 0.2 × 10(6) leukocytes/mL; p < 0.01) of leukocyte migration, while indomethacin inhibited 78.1% (3.2 ± 0.1 × 10(6) leukocytes/mL; p < 0.01). Based on the results obtained in this study, we conclude that BT-MeOH has peripheral antinociceptive and anti-inflammatory activities. However, more studies need to be conducted to confirm these properties. MDPI 2012-09-17 /pmc/articles/PMC3475267/ /pubmed/23118715 http://dx.doi.org/10.3390/md10091977 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Cavalcante-Silva, Luiz Henrique Agra
da Matta, Carolina Barbosa Brito
de Araújo, Morgana Vital
Barbosa-Filho, José Maria
de Lira, Daysianne Pereira
de Oliveira Santos, Bárbara Viviana
de Miranda, George Emmanuel C.
Alexandre-Moreira, Magna Suzana
Antinociceptive and Anti-Inflammatory Activities of Crude Methanolic Extract of Red Alga Bryothamnion triquetrum
title Antinociceptive and Anti-Inflammatory Activities of Crude Methanolic Extract of Red Alga Bryothamnion triquetrum
title_full Antinociceptive and Anti-Inflammatory Activities of Crude Methanolic Extract of Red Alga Bryothamnion triquetrum
title_fullStr Antinociceptive and Anti-Inflammatory Activities of Crude Methanolic Extract of Red Alga Bryothamnion triquetrum
title_full_unstemmed Antinociceptive and Anti-Inflammatory Activities of Crude Methanolic Extract of Red Alga Bryothamnion triquetrum
title_short Antinociceptive and Anti-Inflammatory Activities of Crude Methanolic Extract of Red Alga Bryothamnion triquetrum
title_sort antinociceptive and anti-inflammatory activities of crude methanolic extract of red alga bryothamnion triquetrum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475267/
https://www.ncbi.nlm.nih.gov/pubmed/23118715
http://dx.doi.org/10.3390/md10091977
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