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Down-regulation of TERE1/UBIAD1 activated Ras–MAPK signalling and induced cell proliferation

TERE1/UBIAD1 is involved in SCCD (Schnyder crystalline corneal dystrophy) and multiple human cancers. So far, the molecular mechanism of TERE1/UBIAD1 in tumourigenesis is unclear. Here, the expression levels of hTERT and TERE1/UBIAD1 in pathologically proven Chinese TCC (transitional cell carcinoma)...

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Autores principales: Xia, Yanzhi, Wei, Xiong, Wu, Shimin, Wang, Bo, Wang, Ximing, Hong, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475436/
https://www.ncbi.nlm.nih.gov/pubmed/23119142
http://dx.doi.org/10.1042/CBR20100005
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author Xia, Yanzhi
Wei, Xiong
Wu, Shimin
Wang, Bo
Wang, Ximing
Hong, Ling
author_facet Xia, Yanzhi
Wei, Xiong
Wu, Shimin
Wang, Bo
Wang, Ximing
Hong, Ling
author_sort Xia, Yanzhi
collection PubMed
description TERE1/UBIAD1 is involved in SCCD (Schnyder crystalline corneal dystrophy) and multiple human cancers. So far, the molecular mechanism of TERE1/UBIAD1 in tumourigenesis is unclear. Here, the expression levels of hTERT and TERE1/UBIAD1 in pathologically proven Chinese TCC (transitional cell carcinoma) samples were measured. It was found that decreased TERE1/UBIAD1 expression is closely related to both an increased hTERT expression and activation of Ras–MAPK signalling. Chemically modified TERE1 siRNA oligos were used to knock down TERE1 expression in human L02 cells. Cells transfected with TERE1 siRNA oligos underwent significant cell proliferation. When the levels of hTERT expression and ERK phosphorylation were measured, it was found that both of them increased in the above transfected cells, suggesting the activation of Ras–MAPK signalling. Addition of the MEK inhibitor U0126 into the transfected L02 cells described above inhibited ERK phosphorylation and hTERT expression. Our result is the initial demonstration that down-regulation of TERE1 activates Ras–MAPK signalling and induces subsequent cell proliferation. TERE1 might be a new negative regulator of Ras–MAPK signalling, which plays a pivotal role in the cell proliferation of multiple human cancers.
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spelling pubmed-34754362012-10-30 Down-regulation of TERE1/UBIAD1 activated Ras–MAPK signalling and induced cell proliferation Xia, Yanzhi Wei, Xiong Wu, Shimin Wang, Bo Wang, Ximing Hong, Ling Cell Biol Int Rep (2010) Research Article TERE1/UBIAD1 is involved in SCCD (Schnyder crystalline corneal dystrophy) and multiple human cancers. So far, the molecular mechanism of TERE1/UBIAD1 in tumourigenesis is unclear. Here, the expression levels of hTERT and TERE1/UBIAD1 in pathologically proven Chinese TCC (transitional cell carcinoma) samples were measured. It was found that decreased TERE1/UBIAD1 expression is closely related to both an increased hTERT expression and activation of Ras–MAPK signalling. Chemically modified TERE1 siRNA oligos were used to knock down TERE1 expression in human L02 cells. Cells transfected with TERE1 siRNA oligos underwent significant cell proliferation. When the levels of hTERT expression and ERK phosphorylation were measured, it was found that both of them increased in the above transfected cells, suggesting the activation of Ras–MAPK signalling. Addition of the MEK inhibitor U0126 into the transfected L02 cells described above inhibited ERK phosphorylation and hTERT expression. Our result is the initial demonstration that down-regulation of TERE1 activates Ras–MAPK signalling and induces subsequent cell proliferation. TERE1 might be a new negative regulator of Ras–MAPK signalling, which plays a pivotal role in the cell proliferation of multiple human cancers. Portland Press Ltd 2010-11-08 /pmc/articles/PMC3475436/ /pubmed/23119142 http://dx.doi.org/10.1042/CBR20100005 Text en © The Author(s). http://creativecommons.org/licenses/by-nc/2.5/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commerical use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xia, Yanzhi
Wei, Xiong
Wu, Shimin
Wang, Bo
Wang, Ximing
Hong, Ling
Down-regulation of TERE1/UBIAD1 activated Ras–MAPK signalling and induced cell proliferation
title Down-regulation of TERE1/UBIAD1 activated Ras–MAPK signalling and induced cell proliferation
title_full Down-regulation of TERE1/UBIAD1 activated Ras–MAPK signalling and induced cell proliferation
title_fullStr Down-regulation of TERE1/UBIAD1 activated Ras–MAPK signalling and induced cell proliferation
title_full_unstemmed Down-regulation of TERE1/UBIAD1 activated Ras–MAPK signalling and induced cell proliferation
title_short Down-regulation of TERE1/UBIAD1 activated Ras–MAPK signalling and induced cell proliferation
title_sort down-regulation of tere1/ubiad1 activated ras–mapk signalling and induced cell proliferation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475436/
https://www.ncbi.nlm.nih.gov/pubmed/23119142
http://dx.doi.org/10.1042/CBR20100005
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