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Neurogenic inflammation: a study of rat trigeminal ganglion

Calcitonin gene-related peptide (CGRP) is linked to neurogenic inflammation and to migraine. Activation of the trigeminovascular system plays a prominent role during migraine attacks with the release of CGRP. The trigeminal ganglion (TG) contains three main cell types: neurons, satellite glial cells...

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Detalles Bibliográficos
Autores principales: Kristiansen, Kim Anker, Edvinsson, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Milan 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3476228/
https://www.ncbi.nlm.nih.gov/pubmed/20931347
http://dx.doi.org/10.1007/s10194-010-0260-x
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author Kristiansen, Kim Anker
Edvinsson, Lars
author_facet Kristiansen, Kim Anker
Edvinsson, Lars
author_sort Kristiansen, Kim Anker
collection PubMed
description Calcitonin gene-related peptide (CGRP) is linked to neurogenic inflammation and to migraine. Activation of the trigeminovascular system plays a prominent role during migraine attacks with the release of CGRP. The trigeminal ganglion (TG) contains three main cell types: neurons, satellite glial cells (SGC) and Schwann cells; the first two have before been studied in vitro separately. Culture of rat TG provides a method to induce inflammation and the possibility to evaluate the different cell types in the TG simultaneously. We investigated expression levels of various inflammatory cytokines on mRNA level using RT-PCR arrays and qRT-PCR; furthermore expression at protein level was studied using immunohistochemistry. We report that (1) organ culture of the TG is possible with preserved morphology, (2) organ culture is associated with enhanced expression of cytokines and mitogen-activated protein kinases (MAPKs) primarily in neurons, (3) CGRP can induce expression of some cytokines and (4) cytokine expression is still upregulated following MAPK pathway inhibition by MEK inhibitor U0126 and pp38 inhibitor SB202192, but the cytokine expression is abolished when co-incubating with the JNK inhibitor SP600125. This method may be of value to examine local TG inflammation, putatively involved in the pathophysiology of some forms of primary headaches.
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spelling pubmed-34762282012-11-29 Neurogenic inflammation: a study of rat trigeminal ganglion Kristiansen, Kim Anker Edvinsson, Lars J Headache Pain Original Article Calcitonin gene-related peptide (CGRP) is linked to neurogenic inflammation and to migraine. Activation of the trigeminovascular system plays a prominent role during migraine attacks with the release of CGRP. The trigeminal ganglion (TG) contains three main cell types: neurons, satellite glial cells (SGC) and Schwann cells; the first two have before been studied in vitro separately. Culture of rat TG provides a method to induce inflammation and the possibility to evaluate the different cell types in the TG simultaneously. We investigated expression levels of various inflammatory cytokines on mRNA level using RT-PCR arrays and qRT-PCR; furthermore expression at protein level was studied using immunohistochemistry. We report that (1) organ culture of the TG is possible with preserved morphology, (2) organ culture is associated with enhanced expression of cytokines and mitogen-activated protein kinases (MAPKs) primarily in neurons, (3) CGRP can induce expression of some cytokines and (4) cytokine expression is still upregulated following MAPK pathway inhibition by MEK inhibitor U0126 and pp38 inhibitor SB202192, but the cytokine expression is abolished when co-incubating with the JNK inhibitor SP600125. This method may be of value to examine local TG inflammation, putatively involved in the pathophysiology of some forms of primary headaches. Springer Milan 2010-10-08 2010-12 /pmc/articles/PMC3476228/ /pubmed/20931347 http://dx.doi.org/10.1007/s10194-010-0260-x Text en © Springer-Verlag 2010
spellingShingle Original Article
Kristiansen, Kim Anker
Edvinsson, Lars
Neurogenic inflammation: a study of rat trigeminal ganglion
title Neurogenic inflammation: a study of rat trigeminal ganglion
title_full Neurogenic inflammation: a study of rat trigeminal ganglion
title_fullStr Neurogenic inflammation: a study of rat trigeminal ganglion
title_full_unstemmed Neurogenic inflammation: a study of rat trigeminal ganglion
title_short Neurogenic inflammation: a study of rat trigeminal ganglion
title_sort neurogenic inflammation: a study of rat trigeminal ganglion
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3476228/
https://www.ncbi.nlm.nih.gov/pubmed/20931347
http://dx.doi.org/10.1007/s10194-010-0260-x
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