Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles

Thiol group functionalized silica-coated magnetic nanoparticles (Si-MNPs@SH) were synthesized for rapid and selective magnetic field-based separation of mixed proteins. The highest adsorption efficiencies of binary proteins, bovine serum albumin (BSA; 66 kDa; pI = 4.65) and lysozyme (LYZ; 14.3 kDa;...

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Detalles Bibliográficos
Autores principales: Lee, Soo Youn, Ahn, Chi Young, Lee, Jiho, Lee, Jin Hyung, Chang, Jeong Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2012
Materias:
Nano Express
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3476439/
https://www.ncbi.nlm.nih.gov/pubmed/22650609
http://dx.doi.org/10.1186/1556-276X-7-279
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author Lee, Soo Youn
Ahn, Chi Young
Lee, Jiho
Lee, Jin Hyung
Chang, Jeong Ho
author_facet Lee, Soo Youn
Ahn, Chi Young
Lee, Jiho
Lee, Jin Hyung
Chang, Jeong Ho
author_sort Lee, Soo Youn
collection PubMed
description Thiol group functionalized silica-coated magnetic nanoparticles (Si-MNPs@SH) were synthesized for rapid and selective magnetic field-based separation of mixed proteins. The highest adsorption efficiencies of binary proteins, bovine serum albumin (BSA; 66 kDa; pI = 4.65) and lysozyme (LYZ; 14.3 kDa; pI = 11) were shown at the pH values corresponding to their own pI in the single-component protein. In the mixed protein, however, the adsorption performance of BSA and LYZ by Si-MNPs@SH was governed not only by pH but also by the molecular weight of each protein in the mixed protein.
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spelling pubmed-34764392012-12-05 Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles Lee, Soo Youn Ahn, Chi Young Lee, Jiho Lee, Jin Hyung Chang, Jeong Ho Nanoscale Res Lett Nano Express Thiol group functionalized silica-coated magnetic nanoparticles (Si-MNPs@SH) were synthesized for rapid and selective magnetic field-based separation of mixed proteins. The highest adsorption efficiencies of binary proteins, bovine serum albumin (BSA; 66 kDa; pI = 4.65) and lysozyme (LYZ; 14.3 kDa; pI = 11) were shown at the pH values corresponding to their own pI in the single-component protein. In the mixed protein, however, the adsorption performance of BSA and LYZ by Si-MNPs@SH was governed not only by pH but also by the molecular weight of each protein in the mixed protein. Springer 2012-05-31 /pmc/articles/PMC3476439/ /pubmed/22650609 http://dx.doi.org/10.1186/1556-276X-7-279 Text en Copyright ©2012 Lee et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Nano Express
Lee, Soo Youn
Ahn, Chi Young
Lee, Jiho
Lee, Jin Hyung
Chang, Jeong Ho
Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles
title Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles
title_full Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles
title_fullStr Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles
title_full_unstemmed Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles
title_short Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles
title_sort rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3476439/
https://www.ncbi.nlm.nih.gov/pubmed/22650609
http://dx.doi.org/10.1186/1556-276X-7-279
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