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Tools to kill: Genome of one of the most destructive plant pathogenic fungi Macrophomina phaseolina

BACKGROUND: Macrophomina phaseolina is one of the most destructive necrotrophic fungal pathogens that infect more than 500 plant species throughout the world. It can grow rapidly in infected plants and subsequently produces a large amount of sclerotia that plugs the vessels, resulting in wilting of...

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Autores principales: Islam, Md Shahidul, Haque, Md Samiul, Islam, Mohammad Moinul, Emdad, Emdadul Mannan, Halim, Abdul, Hossen, Quazi Md Mosaddeque, Hossain, Md Zakir, Ahmed, Borhan, Rahim, Sifatur, Rahman, Md Sharifur, Alam, Md Monjurul, Hou, Shaobin, Wan, Xuehua, Saito, Jennifer A, Alam, Maqsudul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3477038/
https://www.ncbi.nlm.nih.gov/pubmed/22992219
http://dx.doi.org/10.1186/1471-2164-13-493
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author Islam, Md Shahidul
Haque, Md Samiul
Islam, Mohammad Moinul
Emdad, Emdadul Mannan
Halim, Abdul
Hossen, Quazi Md Mosaddeque
Hossain, Md Zakir
Ahmed, Borhan
Rahim, Sifatur
Rahman, Md Sharifur
Alam, Md Monjurul
Hou, Shaobin
Wan, Xuehua
Saito, Jennifer A
Alam, Maqsudul
author_facet Islam, Md Shahidul
Haque, Md Samiul
Islam, Mohammad Moinul
Emdad, Emdadul Mannan
Halim, Abdul
Hossen, Quazi Md Mosaddeque
Hossain, Md Zakir
Ahmed, Borhan
Rahim, Sifatur
Rahman, Md Sharifur
Alam, Md Monjurul
Hou, Shaobin
Wan, Xuehua
Saito, Jennifer A
Alam, Maqsudul
author_sort Islam, Md Shahidul
collection PubMed
description BACKGROUND: Macrophomina phaseolina is one of the most destructive necrotrophic fungal pathogens that infect more than 500 plant species throughout the world. It can grow rapidly in infected plants and subsequently produces a large amount of sclerotia that plugs the vessels, resulting in wilting of the plant. RESULTS: We sequenced and assembled ~49 Mb into 15 super-scaffolds covering 92.83% of the M. phaseolina genome. We predict 14,249 open reading frames (ORFs) of which 9,934 are validated by the transcriptome. This phytopathogen has an abundance of secreted oxidases, peroxidases, and hydrolytic enzymes for degrading cell wall polysaccharides and lignocelluloses to penetrate into the host tissue. To overcome the host plant defense response, M. phaseolina encodes a significant number of P450s, MFS type membrane transporters, glycosidases, transposases, and secondary metabolites in comparison to all sequenced ascomycete species. A strikingly distinct set of carbohydrate esterases (CE) are present in M. phaseolina, with the CE9 and CE10 families remarkably higher than any other fungi. The phenotypic microarray data indicates that M. phaseolina can adapt to a wide range of osmotic and pH environments. As a broad host range pathogen, M. phaseolina possesses a large number of pathogen-host interaction genes including those for adhesion, signal transduction, cell wall breakdown, purine biosynthesis, and potent mycotoxin patulin. CONCLUSIONS: The M. phaseolina genome provides a framework of the infection process at the cytological and molecular level which uses a diverse arsenal of enzymatic and toxin tools to destroy the host plants. Further understanding of the M. phaseolina genome-based plant-pathogen interactions will be instrumental in designing rational strategies for disease control, essential to ensuring global agricultural crop production and security.
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spelling pubmed-34770382012-10-20 Tools to kill: Genome of one of the most destructive plant pathogenic fungi Macrophomina phaseolina Islam, Md Shahidul Haque, Md Samiul Islam, Mohammad Moinul Emdad, Emdadul Mannan Halim, Abdul Hossen, Quazi Md Mosaddeque Hossain, Md Zakir Ahmed, Borhan Rahim, Sifatur Rahman, Md Sharifur Alam, Md Monjurul Hou, Shaobin Wan, Xuehua Saito, Jennifer A Alam, Maqsudul BMC Genomics Research Article BACKGROUND: Macrophomina phaseolina is one of the most destructive necrotrophic fungal pathogens that infect more than 500 plant species throughout the world. It can grow rapidly in infected plants and subsequently produces a large amount of sclerotia that plugs the vessels, resulting in wilting of the plant. RESULTS: We sequenced and assembled ~49 Mb into 15 super-scaffolds covering 92.83% of the M. phaseolina genome. We predict 14,249 open reading frames (ORFs) of which 9,934 are validated by the transcriptome. This phytopathogen has an abundance of secreted oxidases, peroxidases, and hydrolytic enzymes for degrading cell wall polysaccharides and lignocelluloses to penetrate into the host tissue. To overcome the host plant defense response, M. phaseolina encodes a significant number of P450s, MFS type membrane transporters, glycosidases, transposases, and secondary metabolites in comparison to all sequenced ascomycete species. A strikingly distinct set of carbohydrate esterases (CE) are present in M. phaseolina, with the CE9 and CE10 families remarkably higher than any other fungi. The phenotypic microarray data indicates that M. phaseolina can adapt to a wide range of osmotic and pH environments. As a broad host range pathogen, M. phaseolina possesses a large number of pathogen-host interaction genes including those for adhesion, signal transduction, cell wall breakdown, purine biosynthesis, and potent mycotoxin patulin. CONCLUSIONS: The M. phaseolina genome provides a framework of the infection process at the cytological and molecular level which uses a diverse arsenal of enzymatic and toxin tools to destroy the host plants. Further understanding of the M. phaseolina genome-based plant-pathogen interactions will be instrumental in designing rational strategies for disease control, essential to ensuring global agricultural crop production and security. BioMed Central 2012-09-19 /pmc/articles/PMC3477038/ /pubmed/22992219 http://dx.doi.org/10.1186/1471-2164-13-493 Text en Copyright ©2012 Islam et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Islam, Md Shahidul
Haque, Md Samiul
Islam, Mohammad Moinul
Emdad, Emdadul Mannan
Halim, Abdul
Hossen, Quazi Md Mosaddeque
Hossain, Md Zakir
Ahmed, Borhan
Rahim, Sifatur
Rahman, Md Sharifur
Alam, Md Monjurul
Hou, Shaobin
Wan, Xuehua
Saito, Jennifer A
Alam, Maqsudul
Tools to kill: Genome of one of the most destructive plant pathogenic fungi Macrophomina phaseolina
title Tools to kill: Genome of one of the most destructive plant pathogenic fungi Macrophomina phaseolina
title_full Tools to kill: Genome of one of the most destructive plant pathogenic fungi Macrophomina phaseolina
title_fullStr Tools to kill: Genome of one of the most destructive plant pathogenic fungi Macrophomina phaseolina
title_full_unstemmed Tools to kill: Genome of one of the most destructive plant pathogenic fungi Macrophomina phaseolina
title_short Tools to kill: Genome of one of the most destructive plant pathogenic fungi Macrophomina phaseolina
title_sort tools to kill: genome of one of the most destructive plant pathogenic fungi macrophomina phaseolina
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3477038/
https://www.ncbi.nlm.nih.gov/pubmed/22992219
http://dx.doi.org/10.1186/1471-2164-13-493
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