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Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases

The retention of fifty structurally different compounds has been studied using linear solvation energy relationships. Investigations were performed with the use of six various stationary phases with two mobile phases (50/50 % v/v methanol/water and 50/50 % v/v acetonitrile/water). Packing materials...

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Autores principales: Studzińska, S., Buszewski, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3477586/
https://www.ncbi.nlm.nih.gov/pubmed/23125462
http://dx.doi.org/10.1007/s10337-012-2310-9
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author Studzińska, S.
Buszewski, B.
author_facet Studzińska, S.
Buszewski, B.
author_sort Studzińska, S.
collection PubMed
description The retention of fifty structurally different compounds has been studied using linear solvation energy relationships. Investigations were performed with the use of six various stationary phases with two mobile phases (50/50 % v/v methanol/water and 50/50 % v/v acetonitrile/water). Packing materials were home-made and functionalized with octadecyl, alkylamide, cholesterol, alkyl-phosphate and phenyl molecules. This is the first attempt to compare all of these stationary phases synthesized on the same silica gel batch. Therefore, all of them may be compared in more complex and believable way, than it was performed earlier in former investigations. The phase properties (based on Abraham model) were used to the classification of stationary phases according to their interaction properties. The hydrophilic system properties s, a, b indicate stronger interactions between solute and mobile phase for most of the columns. Both e and v cause greater retention as a consequence of preferable interactions with stationary phase by electron pairs and cavity formation as well as hydrophobic bonds. However, alkyl-phosphate phase has different retention properties, as it was expressed by positive sign of s coefficient. It may be concluded that most important parameters influencing the retention of compounds are volume and hydrogen bond acceptor basicity. The LSER coefficients showed also the dependency on the type of organic modifier used as a mobile phase component.
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spelling pubmed-34775862012-10-31 Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases Studzińska, S. Buszewski, B. Chromatographia Original The retention of fifty structurally different compounds has been studied using linear solvation energy relationships. Investigations were performed with the use of six various stationary phases with two mobile phases (50/50 % v/v methanol/water and 50/50 % v/v acetonitrile/water). Packing materials were home-made and functionalized with octadecyl, alkylamide, cholesterol, alkyl-phosphate and phenyl molecules. This is the first attempt to compare all of these stationary phases synthesized on the same silica gel batch. Therefore, all of them may be compared in more complex and believable way, than it was performed earlier in former investigations. The phase properties (based on Abraham model) were used to the classification of stationary phases according to their interaction properties. The hydrophilic system properties s, a, b indicate stronger interactions between solute and mobile phase for most of the columns. Both e and v cause greater retention as a consequence of preferable interactions with stationary phase by electron pairs and cavity formation as well as hydrophobic bonds. However, alkyl-phosphate phase has different retention properties, as it was expressed by positive sign of s coefficient. It may be concluded that most important parameters influencing the retention of compounds are volume and hydrogen bond acceptor basicity. The LSER coefficients showed also the dependency on the type of organic modifier used as a mobile phase component. Springer-Verlag 2012-09-15 2012 /pmc/articles/PMC3477586/ /pubmed/23125462 http://dx.doi.org/10.1007/s10337-012-2310-9 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original
Studzińska, S.
Buszewski, B.
Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases
title Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases
title_full Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases
title_fullStr Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases
title_full_unstemmed Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases
title_short Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases
title_sort linear solvation energy relationships in the determination of specificity and selectivity of stationary phases
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3477586/
https://www.ncbi.nlm.nih.gov/pubmed/23125462
http://dx.doi.org/10.1007/s10337-012-2310-9
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