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Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases
The retention of fifty structurally different compounds has been studied using linear solvation energy relationships. Investigations were performed with the use of six various stationary phases with two mobile phases (50/50 % v/v methanol/water and 50/50 % v/v acetonitrile/water). Packing materials...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3477586/ https://www.ncbi.nlm.nih.gov/pubmed/23125462 http://dx.doi.org/10.1007/s10337-012-2310-9 |
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author | Studzińska, S. Buszewski, B. |
author_facet | Studzińska, S. Buszewski, B. |
author_sort | Studzińska, S. |
collection | PubMed |
description | The retention of fifty structurally different compounds has been studied using linear solvation energy relationships. Investigations were performed with the use of six various stationary phases with two mobile phases (50/50 % v/v methanol/water and 50/50 % v/v acetonitrile/water). Packing materials were home-made and functionalized with octadecyl, alkylamide, cholesterol, alkyl-phosphate and phenyl molecules. This is the first attempt to compare all of these stationary phases synthesized on the same silica gel batch. Therefore, all of them may be compared in more complex and believable way, than it was performed earlier in former investigations. The phase properties (based on Abraham model) were used to the classification of stationary phases according to their interaction properties. The hydrophilic system properties s, a, b indicate stronger interactions between solute and mobile phase for most of the columns. Both e and v cause greater retention as a consequence of preferable interactions with stationary phase by electron pairs and cavity formation as well as hydrophobic bonds. However, alkyl-phosphate phase has different retention properties, as it was expressed by positive sign of s coefficient. It may be concluded that most important parameters influencing the retention of compounds are volume and hydrogen bond acceptor basicity. The LSER coefficients showed also the dependency on the type of organic modifier used as a mobile phase component. |
format | Online Article Text |
id | pubmed-3477586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-34775862012-10-31 Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases Studzińska, S. Buszewski, B. Chromatographia Original The retention of fifty structurally different compounds has been studied using linear solvation energy relationships. Investigations were performed with the use of six various stationary phases with two mobile phases (50/50 % v/v methanol/water and 50/50 % v/v acetonitrile/water). Packing materials were home-made and functionalized with octadecyl, alkylamide, cholesterol, alkyl-phosphate and phenyl molecules. This is the first attempt to compare all of these stationary phases synthesized on the same silica gel batch. Therefore, all of them may be compared in more complex and believable way, than it was performed earlier in former investigations. The phase properties (based on Abraham model) were used to the classification of stationary phases according to their interaction properties. The hydrophilic system properties s, a, b indicate stronger interactions between solute and mobile phase for most of the columns. Both e and v cause greater retention as a consequence of preferable interactions with stationary phase by electron pairs and cavity formation as well as hydrophobic bonds. However, alkyl-phosphate phase has different retention properties, as it was expressed by positive sign of s coefficient. It may be concluded that most important parameters influencing the retention of compounds are volume and hydrogen bond acceptor basicity. The LSER coefficients showed also the dependency on the type of organic modifier used as a mobile phase component. Springer-Verlag 2012-09-15 2012 /pmc/articles/PMC3477586/ /pubmed/23125462 http://dx.doi.org/10.1007/s10337-012-2310-9 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Studzińska, S. Buszewski, B. Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases |
title | Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases |
title_full | Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases |
title_fullStr | Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases |
title_full_unstemmed | Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases |
title_short | Linear Solvation Energy Relationships in the Determination of Specificity and Selectivity of Stationary Phases |
title_sort | linear solvation energy relationships in the determination of specificity and selectivity of stationary phases |
topic | Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3477586/ https://www.ncbi.nlm.nih.gov/pubmed/23125462 http://dx.doi.org/10.1007/s10337-012-2310-9 |
work_keys_str_mv | AT studzinskas linearsolvationenergyrelationshipsinthedeterminationofspecificityandselectivityofstationaryphases AT buszewskib linearsolvationenergyrelationshipsinthedeterminationofspecificityandselectivityofstationaryphases |