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High-performance method for specific effect on nucleic acids in cells using TiO(2)~DNA nanocomposites

Nanoparticles are used to solve the current drug delivery problem. We present a high-performance method for efficient and selective action on nucleic acid target in cells using unique TiO(2)·PL-DNA nanocomposites (polylysine-containing DNA fragments noncovalently immobilized onto TiO(2) nanoparticle...

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Autores principales: Levina, Asya S., Repkova, Marina N., Ismagilov, Zinfer R., Shikina, Nadezhda V., Malygin, Ernst G., Mazurkova, Natalia A., Zinov'ev, Victor V., Evdokimov, Alexei A., Baiborodin, Sergei I., Zarytova, Valentina F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3477653/
https://www.ncbi.nlm.nih.gov/pubmed/23091696
http://dx.doi.org/10.1038/srep00756
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author Levina, Asya S.
Repkova, Marina N.
Ismagilov, Zinfer R.
Shikina, Nadezhda V.
Malygin, Ernst G.
Mazurkova, Natalia A.
Zinov'ev, Victor V.
Evdokimov, Alexei A.
Baiborodin, Sergei I.
Zarytova, Valentina F.
author_facet Levina, Asya S.
Repkova, Marina N.
Ismagilov, Zinfer R.
Shikina, Nadezhda V.
Malygin, Ernst G.
Mazurkova, Natalia A.
Zinov'ev, Victor V.
Evdokimov, Alexei A.
Baiborodin, Sergei I.
Zarytova, Valentina F.
author_sort Levina, Asya S.
collection PubMed
description Nanoparticles are used to solve the current drug delivery problem. We present a high-performance method for efficient and selective action on nucleic acid target in cells using unique TiO(2)·PL-DNA nanocomposites (polylysine-containing DNA fragments noncovalently immobilized onto TiO(2) nanoparticles capable of transferring DNA). These nanocomposites were used for inhibition of human influenza A (H3N2) virus replication in infected MDCK cells. They showed a low toxicity (TC(50) ≈ 1800 μg/ml) and a high antiviral activity (>99.9% inhibition of the virus replication). The specificity factor (antisense effect) appeared to depend on the delivery system of DNA fragments. This factor for nanocomposites is ten-times higher than for DNA in the presence of lipofectamine. IC(50) for nanocomposites was estimated to be 1.5 μg/ml (30 nM for DNA), so its selectivity index was calculated as ~1200. Thus, the proposed nanocomposites are prospective for therapeutic application.
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spelling pubmed-34776532012-10-22 High-performance method for specific effect on nucleic acids in cells using TiO(2)~DNA nanocomposites Levina, Asya S. Repkova, Marina N. Ismagilov, Zinfer R. Shikina, Nadezhda V. Malygin, Ernst G. Mazurkova, Natalia A. Zinov'ev, Victor V. Evdokimov, Alexei A. Baiborodin, Sergei I. Zarytova, Valentina F. Sci Rep Article Nanoparticles are used to solve the current drug delivery problem. We present a high-performance method for efficient and selective action on nucleic acid target in cells using unique TiO(2)·PL-DNA nanocomposites (polylysine-containing DNA fragments noncovalently immobilized onto TiO(2) nanoparticles capable of transferring DNA). These nanocomposites were used for inhibition of human influenza A (H3N2) virus replication in infected MDCK cells. They showed a low toxicity (TC(50) ≈ 1800 μg/ml) and a high antiviral activity (>99.9% inhibition of the virus replication). The specificity factor (antisense effect) appeared to depend on the delivery system of DNA fragments. This factor for nanocomposites is ten-times higher than for DNA in the presence of lipofectamine. IC(50) for nanocomposites was estimated to be 1.5 μg/ml (30 nM for DNA), so its selectivity index was calculated as ~1200. Thus, the proposed nanocomposites are prospective for therapeutic application. Nature Publishing Group 2012-10-22 /pmc/articles/PMC3477653/ /pubmed/23091696 http://dx.doi.org/10.1038/srep00756 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Levina, Asya S.
Repkova, Marina N.
Ismagilov, Zinfer R.
Shikina, Nadezhda V.
Malygin, Ernst G.
Mazurkova, Natalia A.
Zinov'ev, Victor V.
Evdokimov, Alexei A.
Baiborodin, Sergei I.
Zarytova, Valentina F.
High-performance method for specific effect on nucleic acids in cells using TiO(2)~DNA nanocomposites
title High-performance method for specific effect on nucleic acids in cells using TiO(2)~DNA nanocomposites
title_full High-performance method for specific effect on nucleic acids in cells using TiO(2)~DNA nanocomposites
title_fullStr High-performance method for specific effect on nucleic acids in cells using TiO(2)~DNA nanocomposites
title_full_unstemmed High-performance method for specific effect on nucleic acids in cells using TiO(2)~DNA nanocomposites
title_short High-performance method for specific effect on nucleic acids in cells using TiO(2)~DNA nanocomposites
title_sort high-performance method for specific effect on nucleic acids in cells using tio(2)~dna nanocomposites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3477653/
https://www.ncbi.nlm.nih.gov/pubmed/23091696
http://dx.doi.org/10.1038/srep00756
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