Improving the thermostability of alpha-amylase by combinatorial coevolving-site saturation mutagenesis

BACKGROUND: The generation of focused mutant libraries at hotspot residues is an important strategy in directed protein evolution. Existing methods, such as combinatorial active site testing and residual coupling analysis, depend primarily on the evolutionary conserved information to find the hotspo...

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Detalles Bibliográficos
Autores principales: Wang, Chenghua, Huang, Ribo, He, Bingfang, Du, Qishi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478181/
https://www.ncbi.nlm.nih.gov/pubmed/23057711
http://dx.doi.org/10.1186/1471-2105-13-263

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478181/
https://www.ncbi.nlm.nih.gov/pubmed/23057711
http://dx.doi.org/10.1186/1471-2105-13-263

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