Cargando…
Improving the thermostability of alpha-amylase by combinatorial coevolving-site saturation mutagenesis
BACKGROUND: The generation of focused mutant libraries at hotspot residues is an important strategy in directed protein evolution. Existing methods, such as combinatorial active site testing and residual coupling analysis, depend primarily on the evolutionary conserved information to find the hotspo...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2012
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478181/ https://www.ncbi.nlm.nih.gov/pubmed/23057711 http://dx.doi.org/10.1186/1471-2105-13-263 |