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In Vitro Osteogenic Properties of Two Dental Implant Surfaces

Current dental implant research aims at understanding the biological basis for successful implant therapy. The aim of the study was to perform a full characterization of the effect of two commercial titanium (Ti) surfaces, OsseoSpeed and TiOblast, on the behaviour of mouse preosteoblast MC3T3-E1 cel...

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Autores principales: Monjo, Marta, Petzold, Christiane, Ramis, Joana Maria, Lyngstadaas, Staale Petter, Ellingsen, Jan Eirik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478747/
https://www.ncbi.nlm.nih.gov/pubmed/23118752
http://dx.doi.org/10.1155/2012/181024
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author Monjo, Marta
Petzold, Christiane
Ramis, Joana Maria
Lyngstadaas, Staale Petter
Ellingsen, Jan Eirik
author_facet Monjo, Marta
Petzold, Christiane
Ramis, Joana Maria
Lyngstadaas, Staale Petter
Ellingsen, Jan Eirik
author_sort Monjo, Marta
collection PubMed
description Current dental implant research aims at understanding the biological basis for successful implant therapy. The aim of the study was to perform a full characterization of the effect of two commercial titanium (Ti) surfaces, OsseoSpeed and TiOblast, on the behaviour of mouse preosteoblast MC3T3-E1 cells. The effect of these Ti surfaces was compared with tissue culture plastic (TCP). In vitro experiments were performed to evaluate cytotoxicity, cell morphology and proliferation, alkaline phosphatase activity, gene expression, and release of a wide array of osteoblast markers. No differences were observed on cell viability and cell proliferation. However, changes were observed in cell shape after 2 days, with a more branched morphology on OsseoSpeed compared to TiOblast. Moreover, OsseoSpeed surface increased BMP-2 secretion after 2 days, and this was followed by increased IGF-I, BSP, and osterix gene expression and mineralization compared to TiOblast after 14 days. As compared to the gold standard TCP, both Ti surfaces induced higher osteocalcin and OPG release than TCP and differential temporal gene expression of osteogenic markers. The results demonstrate that the gain of using OsseoSpeed surface is an improved osteoblast differentiation and mineralization, without additional effects on cell viability or proliferation.
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spelling pubmed-34787472012-11-01 In Vitro Osteogenic Properties of Two Dental Implant Surfaces Monjo, Marta Petzold, Christiane Ramis, Joana Maria Lyngstadaas, Staale Petter Ellingsen, Jan Eirik Int J Biomater Research Article Current dental implant research aims at understanding the biological basis for successful implant therapy. The aim of the study was to perform a full characterization of the effect of two commercial titanium (Ti) surfaces, OsseoSpeed and TiOblast, on the behaviour of mouse preosteoblast MC3T3-E1 cells. The effect of these Ti surfaces was compared with tissue culture plastic (TCP). In vitro experiments were performed to evaluate cytotoxicity, cell morphology and proliferation, alkaline phosphatase activity, gene expression, and release of a wide array of osteoblast markers. No differences were observed on cell viability and cell proliferation. However, changes were observed in cell shape after 2 days, with a more branched morphology on OsseoSpeed compared to TiOblast. Moreover, OsseoSpeed surface increased BMP-2 secretion after 2 days, and this was followed by increased IGF-I, BSP, and osterix gene expression and mineralization compared to TiOblast after 14 days. As compared to the gold standard TCP, both Ti surfaces induced higher osteocalcin and OPG release than TCP and differential temporal gene expression of osteogenic markers. The results demonstrate that the gain of using OsseoSpeed surface is an improved osteoblast differentiation and mineralization, without additional effects on cell viability or proliferation. Hindawi Publishing Corporation 2012 2012-10-15 /pmc/articles/PMC3478747/ /pubmed/23118752 http://dx.doi.org/10.1155/2012/181024 Text en Copyright © 2012 Marta Monjo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Monjo, Marta
Petzold, Christiane
Ramis, Joana Maria
Lyngstadaas, Staale Petter
Ellingsen, Jan Eirik
In Vitro Osteogenic Properties of Two Dental Implant Surfaces
title In Vitro Osteogenic Properties of Two Dental Implant Surfaces
title_full In Vitro Osteogenic Properties of Two Dental Implant Surfaces
title_fullStr In Vitro Osteogenic Properties of Two Dental Implant Surfaces
title_full_unstemmed In Vitro Osteogenic Properties of Two Dental Implant Surfaces
title_short In Vitro Osteogenic Properties of Two Dental Implant Surfaces
title_sort in vitro osteogenic properties of two dental implant surfaces
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478747/
https://www.ncbi.nlm.nih.gov/pubmed/23118752
http://dx.doi.org/10.1155/2012/181024
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