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Dietary Protein Affects Gene Expression and Prevents Lipid Accumulation in the Liver in Mice
BACKGROUND AND AIMS: High protein (HP) diets are suggested to positively modulate obesity and associated increased prevalence of non-alcoholic fatty liver (NAFLD) disease in humans and rodents. The aim of our study was to detect mechanisms by which a HP diet affects hepatic lipid accumulation. METHO...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479095/ https://www.ncbi.nlm.nih.gov/pubmed/23110065 http://dx.doi.org/10.1371/journal.pone.0047303 |
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author | Schwarz, Jessica Tomé, Daniel Baars, Annemarie Hooiveld, Guido J. E. J. Müller, Michael |
author_facet | Schwarz, Jessica Tomé, Daniel Baars, Annemarie Hooiveld, Guido J. E. J. Müller, Michael |
author_sort | Schwarz, Jessica |
collection | PubMed |
description | BACKGROUND AND AIMS: High protein (HP) diets are suggested to positively modulate obesity and associated increased prevalence of non-alcoholic fatty liver (NAFLD) disease in humans and rodents. The aim of our study was to detect mechanisms by which a HP diet affects hepatic lipid accumulation. METHODS: To investigate the acute and long term effect of high protein ingestion on hepatic lipid accumulation under both low and high fat (HF) conditions, mice were fed combinations of high (35 energy%) or low (10 energy%) fat and high (50 energy%) or normal (15 energy%) protein diets for 1 or 12 weeks. Effects on body composition, liver fat, VLDL production rate and the hepatic transcriptome were investigated. RESULTS: Mice fed the HP diets displayed a lower body weight, developed less adiposity and decreased hepatic lipid accumulation, which could be attributed to a combination of several processes. Next to an increased hepatic VLDL production rate, increased energy utilisation due to enhanced protein catabolic processes, such as transamination, TCA cycle and oxidative phosphorylation was found upon high protein ingestion. CONCLUSION: Feeding a HP diet prevented the development of NAFLD by enhancing lipid secretion into VLDL particles and a less efficient use of ingested calories. |
format | Online Article Text |
id | pubmed-3479095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34790952012-10-29 Dietary Protein Affects Gene Expression and Prevents Lipid Accumulation in the Liver in Mice Schwarz, Jessica Tomé, Daniel Baars, Annemarie Hooiveld, Guido J. E. J. Müller, Michael PLoS One Research Article BACKGROUND AND AIMS: High protein (HP) diets are suggested to positively modulate obesity and associated increased prevalence of non-alcoholic fatty liver (NAFLD) disease in humans and rodents. The aim of our study was to detect mechanisms by which a HP diet affects hepatic lipid accumulation. METHODS: To investigate the acute and long term effect of high protein ingestion on hepatic lipid accumulation under both low and high fat (HF) conditions, mice were fed combinations of high (35 energy%) or low (10 energy%) fat and high (50 energy%) or normal (15 energy%) protein diets for 1 or 12 weeks. Effects on body composition, liver fat, VLDL production rate and the hepatic transcriptome were investigated. RESULTS: Mice fed the HP diets displayed a lower body weight, developed less adiposity and decreased hepatic lipid accumulation, which could be attributed to a combination of several processes. Next to an increased hepatic VLDL production rate, increased energy utilisation due to enhanced protein catabolic processes, such as transamination, TCA cycle and oxidative phosphorylation was found upon high protein ingestion. CONCLUSION: Feeding a HP diet prevented the development of NAFLD by enhancing lipid secretion into VLDL particles and a less efficient use of ingested calories. Public Library of Science 2012-10-23 /pmc/articles/PMC3479095/ /pubmed/23110065 http://dx.doi.org/10.1371/journal.pone.0047303 Text en © 2012 Schwarz et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Schwarz, Jessica Tomé, Daniel Baars, Annemarie Hooiveld, Guido J. E. J. Müller, Michael Dietary Protein Affects Gene Expression and Prevents Lipid Accumulation in the Liver in Mice |
title | Dietary Protein Affects Gene Expression and Prevents Lipid Accumulation in the Liver in Mice |
title_full | Dietary Protein Affects Gene Expression and Prevents Lipid Accumulation in the Liver in Mice |
title_fullStr | Dietary Protein Affects Gene Expression and Prevents Lipid Accumulation in the Liver in Mice |
title_full_unstemmed | Dietary Protein Affects Gene Expression and Prevents Lipid Accumulation in the Liver in Mice |
title_short | Dietary Protein Affects Gene Expression and Prevents Lipid Accumulation in the Liver in Mice |
title_sort | dietary protein affects gene expression and prevents lipid accumulation in the liver in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479095/ https://www.ncbi.nlm.nih.gov/pubmed/23110065 http://dx.doi.org/10.1371/journal.pone.0047303 |
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