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The Metabolic Profile of Long-Lived Drosophila melanogaster

We investigated the age-related changes in the metabolic profile of male Drosophila melanogaster and compared the metabolic profile of flies selected for increased longevity to that of control flies of equal age. We found clear differences in metabolite composition between selection regimes and amon...

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Detalles Bibliográficos
Autores principales: Sarup, Pernille, Pedersen, Simon Metz Mariendal, Nielsen, Niels Chr., Malmendal, Anders, Loeschcke, Volker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479100/
https://www.ncbi.nlm.nih.gov/pubmed/23110072
http://dx.doi.org/10.1371/journal.pone.0047461
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author Sarup, Pernille
Pedersen, Simon Metz Mariendal
Nielsen, Niels Chr.
Malmendal, Anders
Loeschcke, Volker
author_facet Sarup, Pernille
Pedersen, Simon Metz Mariendal
Nielsen, Niels Chr.
Malmendal, Anders
Loeschcke, Volker
author_sort Sarup, Pernille
collection PubMed
description We investigated the age-related changes in the metabolic profile of male Drosophila melanogaster and compared the metabolic profile of flies selected for increased longevity to that of control flies of equal age. We found clear differences in metabolite composition between selection regimes and among age groups. Contrary to results found in a previous study of the transcriptome of these lines the metabolic profile did not show a younger pattern in longevity-selected (LS) flies than in same aged control (C) flies. Rather, many of the metabolites affected by age had levels common to older control individuals in the young LS flies. Furthermore, ageing affected the metabolome in a different LS specific direction. The selection induced difference increased with age. Some metabolites involved in oxidative phosphorylation changed with age highlighting the importance of mitochondrial function in the ageing process. However, these metabolites were not affected by selection for increased longevity, indicating that improvements of mitochondrial function were not involved in the increased lifespan of LS lines. Of the eight metabolites identified as having a significant difference in relative abundance between selection regimes in our study choline, lysine and glucose also show difference among lifespan phenotypes in C. elegans indicating that the correlation between the concentration of these metabolites and longevity was evolutionary conserved. Links between longevity and choline concentration is also found in mice making this metabolite an obvious target for further study.
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spelling pubmed-34791002012-10-29 The Metabolic Profile of Long-Lived Drosophila melanogaster Sarup, Pernille Pedersen, Simon Metz Mariendal Nielsen, Niels Chr. Malmendal, Anders Loeschcke, Volker PLoS One Research Article We investigated the age-related changes in the metabolic profile of male Drosophila melanogaster and compared the metabolic profile of flies selected for increased longevity to that of control flies of equal age. We found clear differences in metabolite composition between selection regimes and among age groups. Contrary to results found in a previous study of the transcriptome of these lines the metabolic profile did not show a younger pattern in longevity-selected (LS) flies than in same aged control (C) flies. Rather, many of the metabolites affected by age had levels common to older control individuals in the young LS flies. Furthermore, ageing affected the metabolome in a different LS specific direction. The selection induced difference increased with age. Some metabolites involved in oxidative phosphorylation changed with age highlighting the importance of mitochondrial function in the ageing process. However, these metabolites were not affected by selection for increased longevity, indicating that improvements of mitochondrial function were not involved in the increased lifespan of LS lines. Of the eight metabolites identified as having a significant difference in relative abundance between selection regimes in our study choline, lysine and glucose also show difference among lifespan phenotypes in C. elegans indicating that the correlation between the concentration of these metabolites and longevity was evolutionary conserved. Links between longevity and choline concentration is also found in mice making this metabolite an obvious target for further study. Public Library of Science 2012-10-23 /pmc/articles/PMC3479100/ /pubmed/23110072 http://dx.doi.org/10.1371/journal.pone.0047461 Text en © 2012 Sarup et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sarup, Pernille
Pedersen, Simon Metz Mariendal
Nielsen, Niels Chr.
Malmendal, Anders
Loeschcke, Volker
The Metabolic Profile of Long-Lived Drosophila melanogaster
title The Metabolic Profile of Long-Lived Drosophila melanogaster
title_full The Metabolic Profile of Long-Lived Drosophila melanogaster
title_fullStr The Metabolic Profile of Long-Lived Drosophila melanogaster
title_full_unstemmed The Metabolic Profile of Long-Lived Drosophila melanogaster
title_short The Metabolic Profile of Long-Lived Drosophila melanogaster
title_sort metabolic profile of long-lived drosophila melanogaster
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479100/
https://www.ncbi.nlm.nih.gov/pubmed/23110072
http://dx.doi.org/10.1371/journal.pone.0047461
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