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Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ

EGS (external guide sequence) technology is a promising approach to designing new antibiotics. EGSs are short antisense oligoribonucleotides that induce RNase P-mediated cleavage of a target RNA by forming a precursor tRNA-like complex. The ftsZ mRNA secondary structure was modeled and EGSs compleme...

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Detalles Bibliográficos
Autores principales: Sala, Carol Davies, Soler-Bistué, Alfonso J. C., Korprapun, Leeann, Zorreguieta, Angeles, Tolmasky, Marcelo E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479136/
https://www.ncbi.nlm.nih.gov/pubmed/23110089
http://dx.doi.org/10.1371/journal.pone.0047690
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author Sala, Carol Davies
Soler-Bistué, Alfonso J. C.
Korprapun, Leeann
Zorreguieta, Angeles
Tolmasky, Marcelo E.
author_facet Sala, Carol Davies
Soler-Bistué, Alfonso J. C.
Korprapun, Leeann
Zorreguieta, Angeles
Tolmasky, Marcelo E.
author_sort Sala, Carol Davies
collection PubMed
description EGS (external guide sequence) technology is a promising approach to designing new antibiotics. EGSs are short antisense oligoribonucleotides that induce RNase P-mediated cleavage of a target RNA by forming a precursor tRNA-like complex. The ftsZ mRNA secondary structure was modeled and EGSs complementary to two regions with high probability of being suitable targets were designed. In vitro reactions showed that EGSs targeting these regions bound ftsZ mRNA and elicited RNase P-mediated cleavage of ftsZ mRNA. A recombinant plasmid, pEGSb1, coding for an EGS that targets region “b” under the control of the T7 promoter was generated. Upon introduction of this plasmid into Escherichia coli BL21(DE3)(pLysS) the transformant strain formed filaments when expression of the EGS was induced. Concomitantly, E. coli harboring pEGSb1 showed a modest but significant inhibition of growth when synthesis of the EGSb1 was induced. Our results indicate that EGS technology could be a viable strategy to generate new antimicrobials targeting ftsZ.
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spelling pubmed-34791362012-10-29 Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ Sala, Carol Davies Soler-Bistué, Alfonso J. C. Korprapun, Leeann Zorreguieta, Angeles Tolmasky, Marcelo E. PLoS One Research Article EGS (external guide sequence) technology is a promising approach to designing new antibiotics. EGSs are short antisense oligoribonucleotides that induce RNase P-mediated cleavage of a target RNA by forming a precursor tRNA-like complex. The ftsZ mRNA secondary structure was modeled and EGSs complementary to two regions with high probability of being suitable targets were designed. In vitro reactions showed that EGSs targeting these regions bound ftsZ mRNA and elicited RNase P-mediated cleavage of ftsZ mRNA. A recombinant plasmid, pEGSb1, coding for an EGS that targets region “b” under the control of the T7 promoter was generated. Upon introduction of this plasmid into Escherichia coli BL21(DE3)(pLysS) the transformant strain formed filaments when expression of the EGS was induced. Concomitantly, E. coli harboring pEGSb1 showed a modest but significant inhibition of growth when synthesis of the EGSb1 was induced. Our results indicate that EGS technology could be a viable strategy to generate new antimicrobials targeting ftsZ. Public Library of Science 2012-10-23 /pmc/articles/PMC3479136/ /pubmed/23110089 http://dx.doi.org/10.1371/journal.pone.0047690 Text en © 2012 Sala et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sala, Carol Davies
Soler-Bistué, Alfonso J. C.
Korprapun, Leeann
Zorreguieta, Angeles
Tolmasky, Marcelo E.
Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ
title Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ
title_full Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ
title_fullStr Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ
title_full_unstemmed Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ
title_short Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ
title_sort inhibition of cell division induced by external guide sequences (egs technology) targeting ftsz
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479136/
https://www.ncbi.nlm.nih.gov/pubmed/23110089
http://dx.doi.org/10.1371/journal.pone.0047690
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