Cargando…

Standardization of House Dust Mite Extracts in Korea

PURPOSE: House dust mites are the most important cause of respiratory allergy in Korea. Standardization of allergen extracts is essential for improving diagnostics and immunotherapeutics. This study was undertaken to evaluate the allergenicity of standardized house dust mite allergen extracts from K...

Descripción completa

Detalles Bibliográficos
Autores principales: Jeong, Kyoung Yong, Choi, Soo-Young, Lee, Jae-Hyun, Lee, In-Yong, Yong, Tai-Soon, Lee, Joo-Shil, Hong, Chein-Soo, Park, Jung-Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479228/
https://www.ncbi.nlm.nih.gov/pubmed/23115731
http://dx.doi.org/10.4168/aair.2012.4.6.346
_version_ 1782247431717519360
author Jeong, Kyoung Yong
Choi, Soo-Young
Lee, Jae-Hyun
Lee, In-Yong
Yong, Tai-Soon
Lee, Joo-Shil
Hong, Chein-Soo
Park, Jung-Won
author_facet Jeong, Kyoung Yong
Choi, Soo-Young
Lee, Jae-Hyun
Lee, In-Yong
Yong, Tai-Soon
Lee, Joo-Shil
Hong, Chein-Soo
Park, Jung-Won
author_sort Jeong, Kyoung Yong
collection PubMed
description PURPOSE: House dust mites are the most important cause of respiratory allergy in Korea. Standardization of allergen extracts is essential for improving diagnostics and immunotherapeutics. This study was undertaken to evaluate the allergenicity of standardized house dust mite allergen extracts from Korean house dust mite isolates. METHODS: Allergen extracts were prepared from cultured Korean house dust mites (Dermatophagoides farinae and D. pteronyssinus). Allergenic activities of Korean house dust mite extracts were compared to standardized extracts from a company in the United States whose allergen concentrations were expressed as Allergy Units (AUs). Specifically, we compared group 1 and 2 major allergens using two-site enzyme-linked immunosorbent assay (ELISA) kits and an in vivo intradermal test. RESULTS: Major allergen concentrations were 17.0 µg/mg (5.0 µg/mg of Der f 1 and 12.0 µg/mg of Der f 2) for a D. farinae extract and 24.0 µg/mg (11.6 µg/mg of Der p 1 and 12.4 µg/mg of Der p 2) for a D. pteronyssinus extract. Using chloramphenicol (CAP) inhibition assays, AUs were 12.5 AU/µg for a D. farinae extract and 12.8 AU/µg for a D. pteronyssinus extract. Allergenic activities were 3- to 4-fold stronger when assessed by intradermal skin tests for in vivo standardization. CONCLUSIONS: Allergen extracts were prepared from Korean house dust mites and the allergenicities of the extracts were estimated using AU measurements. House dust mite extracts prepared in this study could be utilized as a reference material, which will be useful for the development of diagnostic and immunotherapeutic reagents in Korea.
format Online
Article
Text
id pubmed-3479228
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease
record_format MEDLINE/PubMed
spelling pubmed-34792282012-11-01 Standardization of House Dust Mite Extracts in Korea Jeong, Kyoung Yong Choi, Soo-Young Lee, Jae-Hyun Lee, In-Yong Yong, Tai-Soon Lee, Joo-Shil Hong, Chein-Soo Park, Jung-Won Allergy Asthma Immunol Res Original Article PURPOSE: House dust mites are the most important cause of respiratory allergy in Korea. Standardization of allergen extracts is essential for improving diagnostics and immunotherapeutics. This study was undertaken to evaluate the allergenicity of standardized house dust mite allergen extracts from Korean house dust mite isolates. METHODS: Allergen extracts were prepared from cultured Korean house dust mites (Dermatophagoides farinae and D. pteronyssinus). Allergenic activities of Korean house dust mite extracts were compared to standardized extracts from a company in the United States whose allergen concentrations were expressed as Allergy Units (AUs). Specifically, we compared group 1 and 2 major allergens using two-site enzyme-linked immunosorbent assay (ELISA) kits and an in vivo intradermal test. RESULTS: Major allergen concentrations were 17.0 µg/mg (5.0 µg/mg of Der f 1 and 12.0 µg/mg of Der f 2) for a D. farinae extract and 24.0 µg/mg (11.6 µg/mg of Der p 1 and 12.4 µg/mg of Der p 2) for a D. pteronyssinus extract. Using chloramphenicol (CAP) inhibition assays, AUs were 12.5 AU/µg for a D. farinae extract and 12.8 AU/µg for a D. pteronyssinus extract. Allergenic activities were 3- to 4-fold stronger when assessed by intradermal skin tests for in vivo standardization. CONCLUSIONS: Allergen extracts were prepared from Korean house dust mites and the allergenicities of the extracts were estimated using AU measurements. House dust mite extracts prepared in this study could be utilized as a reference material, which will be useful for the development of diagnostic and immunotherapeutic reagents in Korea. The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease 2012-11 2012-04-12 /pmc/articles/PMC3479228/ /pubmed/23115731 http://dx.doi.org/10.4168/aair.2012.4.6.346 Text en Copyright © 2012 The Korean Academy of Asthma, Allergy and Clinical Immunology • The Korean Academy of Pediatric Allergy and Respiratory Disease http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Jeong, Kyoung Yong
Choi, Soo-Young
Lee, Jae-Hyun
Lee, In-Yong
Yong, Tai-Soon
Lee, Joo-Shil
Hong, Chein-Soo
Park, Jung-Won
Standardization of House Dust Mite Extracts in Korea
title Standardization of House Dust Mite Extracts in Korea
title_full Standardization of House Dust Mite Extracts in Korea
title_fullStr Standardization of House Dust Mite Extracts in Korea
title_full_unstemmed Standardization of House Dust Mite Extracts in Korea
title_short Standardization of House Dust Mite Extracts in Korea
title_sort standardization of house dust mite extracts in korea
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479228/
https://www.ncbi.nlm.nih.gov/pubmed/23115731
http://dx.doi.org/10.4168/aair.2012.4.6.346
work_keys_str_mv AT jeongkyoungyong standardizationofhousedustmiteextractsinkorea
AT choisooyoung standardizationofhousedustmiteextractsinkorea
AT leejaehyun standardizationofhousedustmiteextractsinkorea
AT leeinyong standardizationofhousedustmiteextractsinkorea
AT yongtaisoon standardizationofhousedustmiteextractsinkorea
AT leejooshil standardizationofhousedustmiteextractsinkorea
AT hongcheinsoo standardizationofhousedustmiteextractsinkorea
AT parkjungwon standardizationofhousedustmiteextractsinkorea