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Identification of a CO(2) Responsive Regulon in Bordetella
Sensing the environment allows pathogenic bacteria to coordinately regulate gene expression to maximize survival within or outside of a host. Here we show that Bordetella species regulate virulence factor expression in response to carbon dioxide levels that mimic in vivo conditions within the respir...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480411/ https://www.ncbi.nlm.nih.gov/pubmed/23112828 http://dx.doi.org/10.1371/journal.pone.0047635 |
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author | Hester, Sara E. Lui, Minghsun Nicholson, Tracy Nowacki, Daryl Harvill, Eric T. |
author_facet | Hester, Sara E. Lui, Minghsun Nicholson, Tracy Nowacki, Daryl Harvill, Eric T. |
author_sort | Hester, Sara E. |
collection | PubMed |
description | Sensing the environment allows pathogenic bacteria to coordinately regulate gene expression to maximize survival within or outside of a host. Here we show that Bordetella species regulate virulence factor expression in response to carbon dioxide levels that mimic in vivo conditions within the respiratory tract. We found strains of Bordetella bronchiseptica that did not produce adenylate cyclase toxin (ACT) when grown in liquid or solid media with ambient air aeration, but produced ACT and additional antigens when grown in air supplemented to 5% CO(2). Transcriptome analysis and quantitative real time-PCR analysis revealed that strain 761, as well as strain RB50, increased transcription of genes encoding ACT, filamentous hemagglutinin (FHA), pertactin, fimbriae and the type III secretion system in 5% CO(2) conditions, relative to ambient air. Furthermore, transcription of cyaA and fhaB in response to 5% CO(2) was increased even in the absence of BvgS. In vitro analysis also revealed increases in cytotoxicity and adherence when strains were grown in 5% CO(2). The human pathogens B. pertussis and B. parapertussis also increased transcription of several virulence factors when grown in 5% CO(2), indicating that this response is conserved among the classical bordetellae. Together, our data indicate that Bordetella species can sense and respond to physiologically relevant changes in CO(2) concentrations by regulating virulence factors important for colonization, persistence and evasion of the host immune response. |
format | Online Article Text |
id | pubmed-3480411 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34804112012-10-30 Identification of a CO(2) Responsive Regulon in Bordetella Hester, Sara E. Lui, Minghsun Nicholson, Tracy Nowacki, Daryl Harvill, Eric T. PLoS One Research Article Sensing the environment allows pathogenic bacteria to coordinately regulate gene expression to maximize survival within or outside of a host. Here we show that Bordetella species regulate virulence factor expression in response to carbon dioxide levels that mimic in vivo conditions within the respiratory tract. We found strains of Bordetella bronchiseptica that did not produce adenylate cyclase toxin (ACT) when grown in liquid or solid media with ambient air aeration, but produced ACT and additional antigens when grown in air supplemented to 5% CO(2). Transcriptome analysis and quantitative real time-PCR analysis revealed that strain 761, as well as strain RB50, increased transcription of genes encoding ACT, filamentous hemagglutinin (FHA), pertactin, fimbriae and the type III secretion system in 5% CO(2) conditions, relative to ambient air. Furthermore, transcription of cyaA and fhaB in response to 5% CO(2) was increased even in the absence of BvgS. In vitro analysis also revealed increases in cytotoxicity and adherence when strains were grown in 5% CO(2). The human pathogens B. pertussis and B. parapertussis also increased transcription of several virulence factors when grown in 5% CO(2), indicating that this response is conserved among the classical bordetellae. Together, our data indicate that Bordetella species can sense and respond to physiologically relevant changes in CO(2) concentrations by regulating virulence factors important for colonization, persistence and evasion of the host immune response. Public Library of Science 2012-10-24 /pmc/articles/PMC3480411/ /pubmed/23112828 http://dx.doi.org/10.1371/journal.pone.0047635 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Hester, Sara E. Lui, Minghsun Nicholson, Tracy Nowacki, Daryl Harvill, Eric T. Identification of a CO(2) Responsive Regulon in Bordetella |
title | Identification of a CO(2) Responsive Regulon in Bordetella
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title_full | Identification of a CO(2) Responsive Regulon in Bordetella
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title_fullStr | Identification of a CO(2) Responsive Regulon in Bordetella
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title_full_unstemmed | Identification of a CO(2) Responsive Regulon in Bordetella
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title_short | Identification of a CO(2) Responsive Regulon in Bordetella
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title_sort | identification of a co(2) responsive regulon in bordetella |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480411/ https://www.ncbi.nlm.nih.gov/pubmed/23112828 http://dx.doi.org/10.1371/journal.pone.0047635 |
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